RFX7 (NM_022841) Human Tagged Lenti ORF Clone
$2,173.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | RFX7 |
| Synonyms | RFXDC2 |
| Vector | pLenti-C-Myc-DDK |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_022841 |
| ORF Size | 4380 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_022841.5, NP_073752.5 |
| RefSeq Size | 7203 bp |
| RefSeq ORF | 4383 bp |
| Locus ID | 64864 |
| UniProt ID | Q2KHR2 |
| Cytogenetics | 15q21.3 |
| Protein Families | Transcription Factors |
| MW | 157.1 kDa |
| Summary | RFX7 is a member of the regulatory factor X (RFX) family of transcription factors (see RFX1, MIM 600006) (Aftab et al., 2008 PubMed 18673564).supplied by OMIM, Mar 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_022841" in other vectors (8)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC211086 | RFX7 (Myc-DDK-tagged)-Human regulatory factor X, 7 (RFX7) | 10 ug |
$1,873.00
|
|
| RC211086L2 | Lenti ORF clone of Human regulatory factor X, 7 (RFX7), mGFP tagged | 10 ug |
$2,173.00
|
|
| RC211086L3 | Lenti ORF clone of Human regulatory factor X, 7 (RFX7), Myc-DDK-tagged | 10 ug |
$2,173.00
|
|
| RC211086L4 | Lenti ORF clone of Human regulatory factor X, 7 (RFX7), mGFP tagged | 10 ug |
$2,173.00
|
|
| RG211086 | RFX7 (tGFP-tagged) - Human regulatory factor X, 7 (RFX7) | 10 ug |
$2,073.00
|
|
| SC106630 | RFX7 (untagged)-Human regulatory factor X, 7 (RFX7) | 10 ug |
$2,972.00
|
|
| SC318623 | RFX7 (untagged)-Human regulatory factor X, 7 (RFX7) | 10 ug |
$1,999.00
|
|
| SC318624 | RFX7 (untagged)-Human regulatory factor X, 7 (RFX7) | 10 ug |
$1,999.00
|
|
Citations
Regulatory factor X 7 limits Myc activity during B cell activation and suppresses Myc-dependent lymphomagenesis
Fischer, Khameneh, Guerra et al
Nat Immunol (2026) 27 (7), 1490-1501 DOI: 10.1038/s41590-026-02526-2
Fischer, Khameneh, Guerra et al
Nat Immunol (2026) 27 (7), 1490-1501 DOI: 10.1038/s41590-026-02526-2
AI Summary: OriGene’s pLenti-ORF human WT RFX7 with Myc-DDK tag (RC211086L1) was used to express wild-type RFX7 in this study, enabling functional analysis of RFX7 and its truncations in B cell activation and lymphomagenesis.
Snippet: The pLenti-ORF clone of human WT RFX7 with a Myc-DDK tag (C-terminal) construct was purchased from OriGene (RC211086L1). The different truncations of RFX7 were generated by PCR using the Q5 High Fidelity polymerase and subcloned (BamHI–MluI) into pLenti-Myc-DDK vector
The transcription factor Rfx7 limits metabolism of NK cells and promotes their maintenance and immunity
Castro, Chelbi, Niogret et al
Nat. Immunol. (2018) 19 (8), 809-820 DOI: 10.1038/s41590-018-0144-9
Castro, Chelbi, Niogret et al
Nat. Immunol. (2018) 19 (8), 809-820 DOI: 10.1038/s41590-018-0144-9
AI Summary: OriGene’s HEK293T cells were used for transfection experiments in this study to investigate the role of Rfx7 in NK cell metabolism and immunity.
Snippet: from OriGene. HEK293T cells were transfected with PEI reagent at a 1:1.5 (DNA/PEI) ratio
Showing 1-2 of 2 papers.
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