MCM10 (NM_018518) Human Tagged ORF Clone
SKU
RC210997
MCM10 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 10 (MCM10), transcript variant 2
$801.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MCM10 |
| Synonyms | CNA43; DNA43; PRO2249 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_018518 |
| ORF Size | 2625 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_018518.5 |
| RefSeq Size | 4559 bp |
| RefSeq ORF | 2625 bp |
| Locus ID | 55388 |
| UniProt ID | Q7L590 |
| Cytogenetics | 10p13 |
| Protein Families | Stem cell - Pluripotency |
| MW | 98.2 kDa |
| Summary | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_018518" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210997L1 | Lenti ORF clone of Human minichromosome maintenance complex component 10 (MCM10), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,101.00
|
|
| RC210997L2 | Lenti ORF clone of Human minichromosome maintenance complex component 10 (MCM10), transcript variant 2, mGFP tagged | 10 ug |
$1,101.00
|
|
| RC210997L3 | Lenti ORF clone of Human minichromosome maintenance complex component 10 (MCM10), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,101.00
|
|
| RC210997L4 | Lenti ORF clone of Human minichromosome maintenance complex component 10 (MCM10), transcript variant 2, mGFP tagged | 10 ug |
$1,101.00
|
|
| RG210997 | MCM10 (tGFP-tagged) - Human minichromosome maintenance complex component 10 (MCM10), transcript variant 2 | 10 ug |
$789.00
$1,001.00
|
|
| SC111824 | MCM10 (untagged)-Human minichromosome maintenance complex component 10 (MCM10), transcript variant 2 | 10 ug |
$801.00
|
|
Citations
MCM10 compensates for Myc-induced DNA replication stress in breast cancer stem-like cells
Murayama, Takeuchi, Yamawaki et al
bioRxiv (2020)
Murayama, Takeuchi, Yamawaki et al
bioRxiv (2020)
Cited by 2
Ablation of MCM10 using CRISPR/Cas9 restrains the growth and migration of esophageal squamous cell carcinoma cells through inhibition of Akt signaling
Yan, Du, Jia et al
Onco Targets Ther (2018) 11, 3323-3333 DOI: 10.2147/OTT.S157025
Yan, Du, Jia et al
Onco Targets Ther (2018) 11, 3323-3333 DOI: 10.2147/OTT.S157025
AI Summary: OriGene’s pCMV6-Entry vector containing full-length MCM10 cDNA was used to overexpress MCM10 in ESCC cells, enabling the authors to assess the functional effects of MCM10 manipulation on cell growth and migration.
Snippet: efficiency. A plasmid expressing human Cas9 was obtained from Addgene. Full-length MCM10 cDNA in pCMV6-Entry vector was obtained from OriGene Technologies (Rockville, MD, USA). Cell transfection and sorting. EC109
Showing 1-2 of 2 papers.
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