GATA4 (NM_002052) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GATA4 |
| Synonyms | ASD2; TACHD; TOF; VSD1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | |
| ACCN | NM_002052 |
| ORF Size | 1326 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002052.5 |
| RefSeq Size | 3419 bp |
| RefSeq ORF | 1329 bp |
| Locus ID | 2626 |
| UniProt ID | P43694 |
| Cytogenetics | 8p23.1 |
| Protein Families | Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors |
| MW | 44.6 kDa |
| Summary | This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. provided by RefSeq, Apr 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002052" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210945L1 | Lenti ORF clone of Human GATA binding protein 4 (GATA4), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC210945L2 | Lenti ORF clone of Human GATA binding protein 4 (GATA4), mGFP tagged | 10 ug |
$757.00
|
|
| RC210945L3 | Lenti ORF clone of Human GATA binding protein 4 (GATA4), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC210945L4 | Lenti ORF clone of Human GATA binding protein 4 (GATA4), mGFP tagged | 10 ug |
$757.00
|
|
| RG210945 | GATA4 (tGFP-tagged) - Human GATA binding protein 4 (GATA4) | 10 ug |
$489.00
$657.00
|
|
| SC124037 | GATA4 (untagged)-Human GATA binding protein 4 (GATA4) | 10 ug |
$457.00
|
|
Citations
Deciphering Transcriptional Networks during Human Cardiac Development
Canac, Cimarosti, Girardeau et al
Cells (2022) 11 (23) DOI: 10.3390/cells11233915
Canac, Cimarosti, Girardeau et al
Cells (2022) 11 (23) DOI: 10.3390/cells11233915
Cited by 16
AI Summary: In this study, OriGene products, specifically the IRX3 and IRX5 expression plasmids, were used to investigate the roles of these transcription factors in activating and interacting with key cardiac regulators during human heart development.
Analysis of variants in GATA4 and FOG2/ZFPM2 demonstrates benign contribution to 46,XY disorders of sex development
van den Bergen, Robevska, Eggers et al
Mol Genet Genomic Med (2020) 8 (3), e1095 DOI: 10.1002/mgg3.1095
van den Bergen, Robevska, Eggers et al
Mol Genet Genomic Med (2020) 8 (3), e1095 DOI: 10.1002/mgg3.1095
Cited by 33
AI Summary: OriGene products pCMV6-Entry-hGATA4 (RC210945) and pCMV6-Entry-hZFPM2 (RC214338) expression vectors were used as templates for mutagenesis to generate and study the functional effects of GATA4 and ZFPM2 variants in this research.
Snippet: …Mutagenesis was performed using the mammalian expression vector containing the human cDNA ORF for: pCMV6-Entry-hGATA4 (RC210945 [NM_002052.4];from OriGene Technologies Inc.) and pCMV6-Entry-hZFPM2 (RC214338 [NM_012082.3];from OriGene Technologies Inc.) both with a C-terminal Myc-DDK tag. Prior to introducing mutations to the pCMV6-Entry-hGATA4 the vector was modified to remove the Myc-DDK tag and introduce a C-terminal HA tag. …
A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts
Xie, Sun, Du et al
Cell Res. (2019) 29 (9), 696-710 DOI: 10.1038/s41422-019-0196-x
Xie, Sun, Du et al
Cell Res. (2019) 29 (9), 696-710 DOI: 10.1038/s41422-019-0196-x
AI Summary: In this study, human full-length TrueClones™ cDNA clones from OriGene were used as templates to amplify five hepatic transcription factors, which were essential for reprogramming fibroblasts into hepatic progenitor-like cells and functionally competent hepatocytes.
Snippet: Complementary DNA of the five hepatic transcription factors were amplified from human full-length TrueClones (Origene) and inserted into pCDH-EF1-MCS-T2A-Puro (System Biosciences) according to the user’s manual. c-MYC was cloned into an inducible system of Fu-tet-hOct4 (hOct4 was replaced by cMYC).37 FUdeltaGW-rtTA was from Addgene. The oligonucleotides encodi
Lung cancer deficient in the tumor suppressor GATA4 is sensitive to TGFBR1 inhibition
Gao, Hu, Tian et al
Nat Commun (2019) 10 (1), 1665 DOI: 10.1038/s41467-019-09295-7
Gao, Hu, Tian et al
Nat Commun (2019) 10 (1), 1665 DOI: 10.1038/s41467-019-09295-7
AI Summary: In this study, OriGene products including the GATA4 expression plasmid (RC210557) and GATA4 shRNA (TL308448) were used to manipulate GATA4 levels in lung cancer cells, enabling the authors to demonstrate GATA4's role as a tumor suppressor and to investigate the effects of its modulation on cell senescence and therapeutic sensitivity.
Snippet: oles in maintaining the phenotype of a particular tissue type by interacting with the super enhancers8. Not surprisingly, TFs frequently function as TSGs9–12. Despite of the importance of TFs in tumorigenesis and their impact on the response of tumor cells to treatment, a systemic assay of TSG TFs remains to be determined in lung cancer. GATA4 belongs to the zinc finger transcription factor fam
The Use of Foxa2-Overexpressing Adipose Tissue-Derived Stem Cells in a Scaffold System Attenuates Acute Liver Injury
Chae, Jun, Lee et al
Gut Liver (2018) 13 (4), 450-460 DOI: 10.5009/gnl18235
Chae, Jun, Lee et al
Gut Liver (2018) 13 (4), 450-460 DOI: 10.5009/gnl18235
Showing 1-5 of 5 papers.
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