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GPHA2 (NM_130769) Human Tagged ORF Clone

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SKU
RC210893
GPHA2 (Myc-DDK-tagged)-Human glycoprotein hormone alpha 2 (GPHA2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GPHA2
Synonyms A2; GPA2; ZSIG51
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210893 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTATGGCGTCCCCTCAAACCCTGGTCCTCTATCTGCTGGTCCTGGCAGTCACTGAAGCCTGGGGCC
AGGAGGCAGTCATCCCAGGCTGCCACTTGCACCCCTTCAATGTGACAGTGCGAAGTGACCGCCAAGGCAC
CTGCCAGGGCTCCCACGTGGCACAGGCCTGTGTGGGCCACTGTGAGTCCAGCGCCTTCCCTTCTCGGTAC
TCTGTGCTGGTGGCCAGTGGTTACCGACACAACATCACCTCCGTCTCTCAGTGCTGCACCATCAGTGGCC
TGAAGAAGGTCAAAGTACAGCTGCAGTGTGTGGGGAGCCGGAGGGAGGAGCTCGAGATCTTCACGGCCAG
GGCCTGCCAGTGTGACATGTGTCGCCTCTCTCGCTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210893 protein sequence
Red=Cloning site Green=Tags(s)

MPMASPQTLVLYLLVLAVTEAWGQEAVIPGCHLHPFNVTVRSDRQGTCQGSHVAQACVGHCESSAFPSRY
SVLVASGYRHNITSVSQCCTISGLKKVKVQLQCVGSRREELEIFTARACQCDMCRLSRY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_130769
ORF Size 387 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_130769.4
RefSeq Size 774 bp
RefSeq ORF 390 bp
Locus ID 170589
UniProt ID Q96T91
Cytogenetics 11q13.1
Protein Families Secreted Protein
MW 14.2 kDa
Summary GPHA2 is a cystine knot-forming polypeptide and a subunit of the dimeric glycoprotein hormone family (Hsu et al., 2002 PubMed 12089349).supplied by OMIM, Mar 2008
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"NM_130769" in other vectors (4)
SKU Description Size Price
RC210893L3 Lenti ORF clone of Human glycoprotein hormone alpha 2 (GPHA2), Myc-DDK-tagged 10 ug
$450.00
RC210893L4 Lenti ORF clone of Human glycoprotein hormone alpha 2 (GPHA2), mGFP tagged 10 ug
$450.00
RG210893 GPHA2 (tGFP-tagged) - Human glycoprotein hormone alpha 2 (GPHA2) 10 ug
$489.00
SC305899 GPHA2 (untagged)-Human glycoprotein hormone alpha 2 (GPHA2) 10 ug
$165.00

Plasmid Map for RC210893

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210893
Circular map for RC210893

file-alt Citations

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