PCDHGA10 (NM_032090) Human Tagged ORF Clone

SKU
RC210864
PCDHGA10 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 10 (PCDHGA10), transcript variant 2
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$589.00 MSRP $778.00 MSRP $778.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PCDHGA10
Synonyms PCDH-GAMMA-A10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210864 representing NM_032090
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCTCAAAGGAATCGCTCAAAGGAATCAAAGGATTGCAGCGGGCTGGTCCTGCTCTGCCTTTTCT
TCGGGATTCCATGGGAGGCTGGAGCCCGGCAGATCTCCTACTCAATTCCTGAGGAATTAGAGAAAGGCTC
TTTCGTGGGCAACATCTCCAAGGACTTGGGTCTGGCGCCCCGGGAGCTGGCGGAGCGCGGAGTCCGCATA
GTCTCCAGAGGTAGGACGCAGCTTTTCTCTCTGAACCCGCGCAGCGGCAGCTTGATCACCGCGGGCAGGA
TAGACCGGGAGGAGCTCTGCGCTCAGAGCGCGCGGTGCGTGGTGAGTTTTAATATCCTTGTGGAAGACAG
GGTGAAACTTTTTGGGATAGAAATAGAAGTAACTGATATCAATGACAATGCTCCAAAATTCCAAGCAGAA
AATCTAGACGTAAAAATTAATGAAAATGTCGCTGCGGGAATGCGTTTTCCTCTCCCGGAAGCTATTGATC
CGGATGTGGGCGTGAACTCCCTGCAGAGCTATCAGCTCAGCCCCAATAAGCACTTCTCCCTAAGAGTTCA
GAGCCGTGCCAATGGCGTCAAGTACCCGGAGCTGGTACTGGAGCACTCCCTAGATCGCGAGGAAGAGGCC
ATTCACCACCTGGTCCTCACCGCCTCCGACGGGGGTGACCCTCTCCGATCTGGCACTGTCCTTGTCAGTG
TGACTGTCTTCGATGCAAATGACAACGCGCCGGTCTTCACCTTGCCAGAATACCGAGTGAGTGTTCCTGA
GAATTTGCCTGTGGGCACTCAGCTGCTGACAGTCACAGCCACCGACAGGGACGAAGGTGCCAATGGAGAA
GTGACATATTCATTCCGAAAATTACCTGACACGCAATTGTTGAAGTTCCAACTAAACAAATATACTGGAG
AAATAAAAATATCAGAAAATCTAGATTATGAAGAAACCGGTTTCTATGAAATAGAAATACAAGCAGAAGA
TGGAGGAGCATATCTTGCAACTGCAAAAGTGTTGATTACAGTAGAAGATGTAAATGACAACAGTCCAGAG
CTGACCATCACGTCTCTATTTAGTCCAGTGACTGAAGATTCACCTCTGGGAACAGTCGTAGCCCTTTTAA
ATGTGCATGATTTAGACTCTGAGCAGAATGGACAGGTAACCTGTTCCATTTTGGCGTATCTACCATTTAA
ATTAGAAAAGTCCATTGACAGTTATTACAGATTGGTGATACACAGAGCCCTTGACAGGGAACAGGTATCC
TCTTACAATATCACAGTGACAGCCACAGATGGGGGAAGTCCTCCTCTATCAACGGAAGCTCACTTTATGC
TACAAGTGGCAGATATCAATGACAACCCACCTACCTTCTCTCAAGTCTCCTACTTTACCTATATCCCAGA
GAACAACGCCAGGGGTGCCTCCATCTTCTCAGTGACAGCGCTGGACCCGGACAGCAAAGAGAATGCCCAG
ATTATTTACTCCCTGGCTGAAGACACCATCCAGGGGGTACCTCTGTCCTCATACATATCCATCAACTCAG
ACACTGGCGTCCTGTATGCACTCAGATCCTTCGACTATGAGCAGTTTCATGAGCTACAGATGCAGGTGAC
AGCCAGCGACAGCGGGGATCCTCCACTCAGCAGCAACGTGTCGTTGAGCCTGTTTGTGCTGGACCAGAAC
GACAATGCGCCCGAGATCCTGTACCCCGCCCTCCCCACAGACGGTTCCACAGGCGTGGAGCTGGCGCCCC
GCTCCGCAGAGCCCGGCTACCTGGTGACCAAGGTGGTGGCGGTGGACAGAGACTCCGGCCAGAACGCCTG
GCTGTCCTACCGTCTGCTCAAGGCCAGCGAGCCGGGACTCTTCGCGGTGGGGGAGCACACGGGCGAGGTG
CGCACGGCGCGAGCCCTGCTGGACAGAGACGCGCTCAAGCAAAGCCTCGTAGTGGCCGTCCAGGACCACG
GCCAGCCCCCTCTCTCCGCCACTGTCACGCTCACCGTGGCCGTGGCCGACAGCATCCCCCAAGTCCTGGC
GGACCTCGGCAGCTTCGAGTCTCCAGCTAACTCTGAAACCTCAGACCTCACTCTGTACCTGGTGGTAGCG
GTGGCCGCGGTCTCCTGCGTCTTCCTGGCCTTCGTCATCGTGCTGCTGGCGCACAGGCTGCGGCGCTGGC
ACAAGTCACGCCTGCTGCAGGCTTCAGGAGGCGGCTTGACAGGTGTGTCCGGCTCGCACTTTGTGGGCGT
GGACGGGGTTCGGGCTTTCCTGCAGACCTATTCCCACGAGGTCTCTCTCACCGCGGACTCGCGAAAGAGT
CACCTGATCTTCCCCCAGCCCAATTATGCGGACACGCTCATCAGCCAGGAGAGCTGTGAGAAAAACGATC
CTTTGTCTTTGTTAGATGATTCGAAGTTTCCTATAGAGGATACCCCATTGGTTCCAGTGAGTTTTATTTT
CATTTTTACTTTTGTTAAAAAAAAAAAGATTGGTTTTTACTTTGAAGTTTGCGGCATGATGGTGGAAAGT
GTAAATGCTAAAACACTGATGAGTAGAATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210864 representing NM_032090
Red=Cloning site Green=Tags(s)

MAAQRNRSKESKDCSGLVLLCLFFGIPWEAGARQISYSIPEELEKGSFVGNISKDLGLAPRELAERGVRI
VSRGRTQLFSLNPRSGSLITAGRIDREELCAQSARCVVSFNILVEDRVKLFGIEIEVTDINDNAPKFQAE
NLDVKINENVAAGMRFPLPEAIDPDVGVNSLQSYQLSPNKHFSLRVQSRANGVKYPELVLEHSLDREEEA
IHHLVLTASDGGDPLRSGTVLVSVTVFDANDNAPVFTLPEYRVSVPENLPVGTQLLTVTATDRDEGANGE
VTYSFRKLPDTQLLKFQLNKYTGEIKISENLDYEETGFYEIEIQAEDGGAYLATAKVLITVEDVNDNSPE
LTITSLFSPVTEDSPLGTVVALLNVHDLDSEQNGQVTCSILAYLPFKLEKSIDSYYRLVIHRALDREQVS
SYNITVTATDGGSPPLSTEAHFMLQVADINDNPPTFSQVSYFTYIPENNARGASIFSVTALDPDSKENAQ
IIYSLAEDTIQGVPLSSYISINSDTGVLYALRSFDYEQFHELQMQVTASDSGDPPLSSNVSLSLFVLDQN
DNAPEILYPALPTDGSTGVELAPRSAEPGYLVTKVVAVDRDSGQNAWLSYRLLKASEPGLFAVGEHTGEV
RTARALLDRDALKQSLVVAVQDHGQPPLSATVTLTVAVADSIPQVLADLGSFESPANSETSDLTLYLVVA
VAAVSCVFLAFVIVLLAHRLRRWHKSRLLQASGGGLTGVSGSHFVGVDGVRAFLQTYSHEVSLTADSRKS
HLIFPQPNYADTLISQESCEKNDPLSLLDDSKFPIEDTPLVPVSFIFIFTFVKKKKIGFYFEVCGMMVES
VNAKTLMSRI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_032090
ORF Size 2550 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032090.1, NP_114479.1
RefSeq Size 2553 bp
RefSeq ORF 2553 bp
Locus ID 56106
UniProt ID Q9Y5H3
Cytogenetics 5q31.3
Domains CA
MW 92.9 kDa
Summary This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq, Jul 2008]
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SKU Description Size Price
RC210864L3 Lenti-ORF clone of PCDHGA10 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 10 (PCDHGA10), transcript variant 2 10 ug
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RG210864 PCDHGA10 (tGFP-tagged) - Human protocadherin gamma subfamily A, 10 (PCDHGA10), transcript variant 2 10 ug
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$779.00

Citations

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