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PCDHGA10 (NM_032090) Human Untagged Clone

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SKU
SC106447
PCDHGA10 (untagged)-Human protocadherin gamma subfamily A, 10 (PCDHGA10), transcript variant 2
  $779.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PCDHGA10
Synonyms PCDH-GAMMA-A10
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_032090, the custom clone sequence may differ by one or more nucleotides


ATGGCCGCTCAAAGGAATCGCTCAAAGGAATCAAAGGATTGCAGCGGGCTGGTCCTGCTCTGCCTTTTCT
TCGGGATTCCATGGGAGGCTGGAGCCCGGCAGATCTCCTACTCAATTCCTGAGGAATTAGAGAAAGGCTC
TTTCGTGGGCAACATCTCCAAGGACTTGGGTCTGGCGCCCCGGGAGCTGGCGGAGCGCGGAGTCCGCATA
GTCTCCAGAGGTAGGACGCAGCTTTTCTCTCTGAACCCGCGCAGCGGCAGCTTGATCACCGCGGGCAGGA
TAGACCGGGAGGAGCTCTGCGCTCAGAGCGCGCGGTGCGTGGTGAGTTTTAATATCCTTGTGGAAGACAG
GGTGAAACTTTTTGGGATAGAAATAGAAGTAACTGATATCAATGACAATGCTCCAAAATTCCAAGCAGAA
AATCTAGACGTAAAAATTAATGAAAATGTCGCTGCGGGAATGCGTTTTCCTCTCCCGGAAGCTATTGATC
CGGATGTGGGCGTGAACTCCCTGCAGAGCTATCAGCTCAGCCCCAATAAGCACTTCTCCCTAAGAGTTCA
GAGCCGTGCCAATGGCGTCAAGTACCCGGAGCTGGTACTGGAGCACTCCCTAGATCGCGAGGAAGAGGCC
ATTCACCACCTGGTCCTCACCGCCTCCGACGGGGGTGACCCTCTCCGATCTGGCACTGTCCTTGTCAGTG
TGACTGTCTTCGATGCAAATGACAACGCGCCGGTCTTCACCTTGCCAGAATACCGAGTGAGTGTTCCTGA
GAATTTGCCTGTGGGCACTCAGCTGCTGACAGTCACAGCCACCGACAGGGACGAAGGTGCCAATGGAGAA
GTGACATATTCATTCCGAAAATTACCTGACACGCAATTGTTGAAGTTCCAACTAAACAAATATACTGGAG
AAATAAAAATATCAGAAAATCTAGATTATGAAGAAACCGGTTTCTATGAAATAGAAATACAAGCAGAAGA
TGGAGGAGCATATCTTGCAACTGCAAAAGTGTTGATTACAGTAGAAGATGTAAATGACAACAGTCCAGAG
CTGACCATCACGTCTCTATTTAGTCCAGTGACTGAAGATTCACCTCTGGGAACAGTCGTAGCCCTTTTAA
ATGTGCATGATTTAGACTCTGAGCAGAATGGACAGGTAACCTGTTCCATTTTGGCGTATCTACCATTTAA
ATTAGAAAAGTCCATTGACAGTTATTACAGATTGGTGATACACAGAGCCCTTGACAGGGAACAGGTATCC
TCTTACAATATCACAGTGACAGCCACAGATGGGGGAAGTCCTCCTCTATCAACGGAAGCTCACTTTATGC
TACAAGTGGCAGATATCAATGACAACCCACCTACCTTCTCTCAAGTCTCCTACTTTACCTATATCCCAGA
GAACAACGCCAGGGGTGCCTCCATCTTCTCAGTGACAGCGCTGGACCCGGACAGCAAAGAGAATGCCCAG
ATTATTTACTCCCTGGCTGAAGACACCATCCAGGGGGTACCTCTGTCCTCATACATATCCATCAACTCAG
ACACTGGCGTCCTGTATGCACTCAGATCCTTCGACTATGAGCAGTTTCATGAGCTACAGATGCAGGTGAC
AGCCAGCGACAGCGGGGATCCTCCACTCAGCAGCAACGTGTCGTTGAGCCTGTTTGTGCTGGACCAGAAC
GACAATGCGCCCGAGATCCTGTACCCCGCCCTCCCCACAGACGGTTCCACAGGCGTGGAGCTGGCGCCCC
GCTCCGCAGAGCCCGGCTACCTGGTGACCAAGGTGGTGGCGGTGGACAGAGACTCCGGCCAGAACGCCTG
GCTGTCCTACCGTCTGCTCAAGGCCAGCGAGCCGGGACTCTTCGCGGTGGGGGAGCACACGGGCGAGGTG
CGCACGGCGCGAGCCCTGCTGGACAGAGACGCGCTCAAGCAAAGCCTCGTAGTGGCCGTCCAGGACCACG
GCCAGCCCCCTCTCTCCGCCACTGTCACGCTCACCGTGGCCGTGGCCGACAGCATCCCCCAAGTCCTGGC
GGACCTCGGCAGCTTCGAGTCTCCAGCTAACTCTGAAACCTCAGACCTCACTCTGTACCTGGTGGTAGCG
GTGGCCGCGGTCTCCTGCGTCTTCCTGGCCTTCGTCATCGTGCTGCTGGCGCACAGGCTGCGGCGCTGGC
ACAAGTCACGCCTGCTGCAGGCTTCAGGAGGCGGCTTGACAGGTGTGTCCGGCTCGCACTTTGTGGGCGT
GGACGGGGTTCGGGCTTTCCTGCAGACCTATTCCCACGAGGTCTCTCTCACCGCGGACTCGCGAAAGAGT
CACCTGATCTTCCCCCAGCCCAATTATGCGGACACGCTCATCAGCCAGGAGAGCTGTGAGAAAAACGATC
CTTTGTCTTTGTTAGATGATTCGAAGTTTCCTATAGAGGATACCCCATTGGTTCCAGTGAGTTTTATTTT
CATTTTTACTTTTGTTAAAAAAAAAAAGATTGGTTTTTACTTTGAAGTTTGCGGCATGATGGTGGAAAGT
GTAAATGCTAAAACACTGATGAGTAGAATTTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_032090 unedited
ATATCACCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC
GAATTCGGCACGAGGGGCTGCTGGGCTGCAGGGAAGCTCACTCCAGAATTTAAAGTGCCC
AGGCTACAGAGACACCCTGAAGCCACAGAAAGACAAAGGAACCGGTTGAAACACACAACG
TGTCCAGTGAGGACTTTGCAGAATTCTGTAACCAGACTACAATGGCCGCTCAAAGGAATC
GCTCAAAGGAATCAAAGGATTGCAGCGGGCTGGTCCTGCTCTGCCTTTTCTTCGGGATTC
CATGGGAGGCTGGAGCCCGGCAGATCTCCTACTCAATTCCTGAGGAATTAGAGAAAGGCT
CTTTCGTGGGCAACATCTCCAAGGACTTGGGTCTGGCGCCCCGGGAGCTGGCGGAGCGCG
GAGTCCGCATAGTCTCCAGAGGTAGGACGCAGCTTTTCTCTCTGAACCCGCGCAGCGGCA
GCTTGGTCACCGCGGGCAGGATAGACCGGGAGGAGCTCTGCGCTCANAGCGCGCGGTGCG
TGGTGAGTTTTAATATCCTTGTGGAAGACAGGGTGAAACTTTTTGGGATAGAAATAGAAG
TAACTGATATCAATGACAATGCTCCANAATTCCAAGCAGAAAATCTAGACGTAAAAATTA
ATGAAAATGTCGCTGCGGGAATGGCGTTTCCTCTCCCGGAGCTATTGATCCGGATGTGGG
GCGTGAACTCCTGCAGAGCTATCAGCTCAGCCCCAATAGCACTNCTCCCTAAGAAGTCAG
AGGCGTGCCATGGCGTCAGTACCCGAGCTGGACTGGAGCACTCCTAGACGCGAGGAGAGG
CATTCACACTGGTCCTCACCGCTCGAGGGGGTGCCCTCTCGACTGGACTGTCCTGTC
Restriction Sites NotI-NotI |
ACCN NM_032090
Insert Size 4700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032090.1, NP_114479.1
RefSeq Size 2553 bp
RefSeq ORF 2553 bp
Locus ID 56106
UniProt ID Q9Y5H3
Cytogenetics 5q31.3
Domains CA
Summary This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) utilizes the large, first exon then continues into the downstream intron 1 sequence before terminating. This one-exon transcript encodes the shorter isoform (2).
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Documents
Resources
"NM_032090" in other vectors (4)
SKU Description Size Price
RC210864 PCDHGA10 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 10 (PCDHGA10), transcript variant 2 10 ug
$589.00 $778.00
RC210864L3 Lenti-ORF clone of PCDHGA10 (Myc-DDK-tagged)-Human protocadherin gamma subfamily A, 10 (PCDHGA10), transcript variant 2 10 ug
$1,078.00
RC210864L4 Lenti-ORF clone of PCDHGA10 (mGFP-tagged)-Human protocadherin gamma subfamily A, 10 (PCDHGA10), transcript variant 2 10 ug
$1,078.00
RG210864 PCDHGA10 (tGFP-tagged) - Human protocadherin gamma subfamily A, 10 (PCDHGA10), transcript variant 2 10 ug
$978.00

file-alt Citations

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