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EDAR (NM_022336) Human Tagged ORF Clone

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SKU
RC210862
EDAR (Myc-DDK-tagged)-Human ectodysplasin A receptor (EDAR)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
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Other products for "EDAR or NM_022336"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EDAR
Synonyms DL; ECTD10A; ECTD10B; ED1R; ED3; ED5; EDA-A1R; EDA1R; EDA3; HRM1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210862 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCATGTGGGGGACTGCACGCAGACGCCCTGGCTCCCCGTCCTGGTGGTGTCTCTGATGTGCTCAG
CCCGAGCGGAATACTCAAACTGCGGTGAGAACGAGTACTACAACCAGACTACGGGGCTGTGCCAGGAGTG
CCCCCCGTGTGGGCCGGGAGAGGAGCCCTACCTGTCCTGTGGCTACGGCACCAAAGACGAGGACTACGGC
TGCGTCCCCTGCCCGGCGGAGAAGTTTTCCAAAGGAGGCTACCAGATATGCAGGCGTCACAAAGACTGTG
AGGGCTTCTTCCGGGCCACCGTGCTGACACCAGGGGACATGGAGAATGACGCTGAGTGTGGCCCTTGCCT
CCCTGGCTACTACATGCTGGAGAACAGACCGAGGAACATCTATGGCATGGTCTGCTACTCCTGCCTCCTG
GCACCCCCCAACACCAAGGAATGTGTGGGAGCCACTTCAGGAGCTTCTGCCAACTTCCCTGGCACCTCGG
GCAGCAGCACCCTGTCTCCCTTCCAGCACGCCCACAAAGAACTCTCAGGCCAAGGACACCTGGCCACTGC
CCTGATCATTGCAATGTCCACCATCTTCATCATGGCCATCGCCATCGTCCTCATCATCATGTTCTACATC
CTGAAGACAAAGCCCTCTGCCCCAGCCTGTTGCACCAGCCACCCGGGGAAGAGCGTGGAGGCCCAAGTGA
GCAAGGACGAGGAGAAGAAAGAGGCCCCAGACAACGTGGTGATGTTCTCTGAGAAGGATGAATTTGAGAA
GCTGACAGCAACTCCAGCAAAGCCCACCAAGAGCGAGAACGATGCCTCATCCGAGAATGAGCAGCTGCTG
AGCCGGAGCGTCGACAGTGATGAGGAGCCCGCCCCTGACAAGCAGGGCTCCCCGGAGCTGTGCCTGCTGT
CGCTGGTTCACCTGGCCAGGGAGAAGTCTGCCACCAGCAACAAGTCAGCCGGGATTCAAAGCCGGAGGAA
AAAGATCCTCGATGTGTATGCCAACGTGTGTGGAGTCGTGGAAGGTCTTAGCCCCACGGAGCTGCCATTT
GATTGTCTCGAGAAGACTAGCCGAATGCTCAGCTCCACGTACAACTCTGAGAAGGCTGTTGTGAAAACGT
GGCGCCACCTCGCCGAGAGCTTCGGCCTGAAGAGGGATGAGATTGGGGGCATGACAGACGGCATGCAACT
CTTTGACCGCATCAGCACGGCAGGCTACAGCATCCCTGAGCTACTCACAAAACTGGTGCAGATTGAGCGG
CTGGATGCTGTGGAGTCCTTGTGTGCAGACATACTGGAGTGGGCGGGGGTTGTGCCACCTGCCTCCCAGC
CACATGCTGCATCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210862 protein sequence
Red=Cloning site Green=Tags(s)

MAHVGDCTQTPWLPVLVVSLMCSARAEYSNCGENEYYNQTTGLCQECPPCGPGEEPYLSCGYGTKDEDYG
CVPCPAEKFSKGGYQICRRHKDCEGFFRATVLTPGDMENDAECGPCLPGYYMLENRPRNIYGMVCYSCLL
APPNTKECVGATSGASANFPGTSGSSTLSPFQHAHKELSGQGHLATALIIAMSTIFIMAIAIVLIIMFYI
LKTKPSAPACCTSHPGKSVEAQVSKDEEKKEAPDNVVMFSEKDEFEKLTATPAKPTKSENDASSENEQLL
SRSVDSDEEPAPDKQGSPELCLLSLVHLAREKSATSNKSAGIQSRRKKILDVYANVCGVVEGLSPTELPF
DCLEKTSRMLSSTYNSEKAVVKTWRHLAESFGLKRDEIGGMTDGMQLFDRISTAGYSIPELLTKLVQIER
LDAVESLCADILEWAGVVPPASQPHAAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022336
ORF Size 1344 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022336.4
RefSeq Size 4226 bp
RefSeq ORF 1347 bp
Locus ID 10913
UniProt ID Q9UNE0
Cytogenetics 2q13
Domains DEATH
Protein Families Druggable Genome, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction
MW 48.6 kDa
Summary This gene encodes a member of the tumor necrosis factor receptor family. The encoded transmembrane protein is a receptor for the soluble ligand ectodysplasin A, and can activate the nuclear factor-kappaB, JNK, and caspase-independent cell death pathways. It is required for the development of hair, teeth, and other ectodermal derivatives. Mutations in this gene result in autosomal dominant and recessive forms of hypohidrotic ectodermal dysplasia. provided by RefSeq, Jul 2008
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Documents
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"NM_022336" in other vectors (6)
SKU Description Size Price
RC210862L1 Lenti ORF clone of Human ectodysplasin A receptor (EDAR), Myc-DDK-tagged 10 ug
$757.00
RC210862L2 Lenti ORF clone of Human ectodysplasin A receptor (EDAR), mGFP tagged 10 ug
$757.00
RC210862L3 Lenti ORF clone of Human ectodysplasin A receptor (EDAR), Myc-DDK-tagged 10 ug
$757.00
RC210862L4 Lenti ORF clone of Human ectodysplasin A receptor (EDAR), mGFP tagged 10 ug
$757.00
RG210862 EDAR (tGFP-tagged) - Human ectodysplasin A receptor (EDAR) 10 ug
$489.00 $657.00
SC112525 EDAR (untagged)-Human ectodysplasin A receptor (EDAR) 10 ug
$457.00

Plasmid Map for RC210862

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210862
Circular map for RC210862

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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