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MSL2L1 (MSL2) (NM_018133) Human Tagged ORF Clone

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SKU
RC210829
MSL2 (Myc-DDK-tagged)-Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $537.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MSL2L1
Synonyms MSL-2; MSL2L1; RNF184
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210829 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCCCGTGAATGCTACTGCTCTCTACATTTCCGCGAGCCGCCTAGTGCTCAACTACGACCCCGGAG
ACCCCAAGGCGTTTACTGAGATTAACAGGCTCTTGCCTTACTTCCGACAGTCCCTTTCGTGCTGTGTTTG
CGGACATTTGCTACAAGATCCTATTGCACCCACCAACTCCACCTGCCAACATTATGTCTGCAAAACTTGT
AAAGGCAAGAAAATGATGATGAAACCTTCCTGTAGCTGGTGCAAAGACTATGAGCAGTTTGAGGAAAACA
AGCAGTTAAGCATCCTAGTGAACTGCTACAAAAAACTATGCGAGTATATAACACAGACTACACTGGCACG
GGATATAATAGAAGCAGTTGACTGTTCTTCTGATATTTTGGCTTTGCTTAATGATGGATCATTGTTTTGT
GAGGAGACAGAAAAACCCTCAGATTCATCCTTTACTTTGTGTTTGACACATTCCCCTTTACCTTCAACCT
CAGAACCCACAACTGATCCTCAAGCTAGTTTATCTCCAATGTCTGAAAGCACCCTCAGCATTGCTATTGG
CAGTTCTGTTATCAATGGTTTGCCTACTTATAATGGGCTTTCAATAGATAGATTTGGTATAAATATTCCT
TCACCTGAACATTCAAATACGATTGACGTATGTAATACTGTTGACATAAAAACTGAGGATCTGTCTGACA
GCCTGCCACCCGTTTGTGACACAGTAGCCACTGACTTATGTTCCACAGGCATTGATATCTGCAGTTTCAG
TGAAGATATAAAACCTGGAGACTCTCTGTTACTGAGTGTTGAGGAAGTACTCCGCAGCTTAGAAACTGTT
TCAAATACAGAGGTCTGTTGCCCTAATTTGCAGCCGAACTTGGAAGCCACTGTATCCAATGGACCTTTTC
TGCAGCTTTCTTCCCAGTCTCTTAGCCATAATGTTTTTATGTCCACCAGTCCTGCACTTCATGGGTTATC
ATGTACAGCAGCAACTCCGAAGATAGCAAAATTGAATAGAAAACGATCCAGATCAGAGAGTGACAGTGAG
AAAGTTCAGCCACTTCCAATTTCTACCATTATCCGAGGCCCAACACTGGGGGCATCTGCTCCTGTGACAG
TGAAACGGGAGAGCAAAATTTCTCTTCAACCTATAGCAACTGTTCCCAATGGAGGCACAACACCTAAAAT
CAGCAAAACTGTACTTTTATCTACTAAAAGCATGAAAAAGAGTCATGAACATGGATCCAAGAAATCTCAC
TCTAAAACCAAGCCAGGTATTCTTAAAAAAGACAAAGCAGTAAAGGAAAAGATTCCTAGTCATCATTTTA
TGCCAGGAAGTCCTACCAAGACTGTGTACAAAAAACCCCAGGAAAAGAAAGGGTGTAAATGTGGGCGTGC
TACTCAAAATCCAAGTGTTCTTACATGCCGAGGCCAACGCTGCCCTTGCTACTCTAACCGCAAAGCCTGC
TTAGATTGTATATGTCGTGGCTGCCAAAACTCCTATATGGCCAATGGGGAGAAGAAGCTGGAGGCATTTG
CCGTGCCAGAAAAGGCCTTGGAGCAGACCAGGCTCACTTTGGGCATTAACGTGACTAGCATTGCTGTGCG
TAACGCTAGTACCAGCACCAGTGTAATAAATGTCACAGGGTCCCCAGTAACGACGTTTTTAGCTGCCAGT
ACACATGATGATAAAAGTTTGGATGAAGCTATAGACATGAGATTCGACTGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210829 protein sequence
Red=Cloning site Green=Tags(s)

MNPVNATALYISASRLVLNYDPGDPKAFTEINRLLPYFRQSLSCCVCGHLLQDPIAPTNSTCQHYVCKTC
KGKKMMMKPSCSWCKDYEQFEENKQLSILVNCYKKLCEYITQTTLARDIIEAVDCSSDILALLNDGSLFC
EETEKPSDSSFTLCLTHSPLPSTSEPTTDPQASLSPMSESTLSIAIGSSVINGLPTYNGLSIDRFGINIP
SPEHSNTIDVCNTVDIKTEDLSDSLPPVCDTVATDLCSTGIDICSFSEDIKPGDSLLLSVEEVLRSLETV
SNTEVCCPNLQPNLEATVSNGPFLQLSSQSLSHNVFMSTSPALHGLSCTAATPKIAKLNRKRSRSESDSE
KVQPLPISTIIRGPTLGASAPVTVKRESKISLQPIATVPNGGTTPKISKTVLLSTKSMKKSHEHGSKKSH
SKTKPGILKKDKAVKEKIPSHHFMPGSPTKTVYKKPQEKKGCKCGRATQNPSVLTCRGQRCPCYSNRKAC
LDCICRGCQNSYMANGEKKLEAFAVPEKALEQTRLTLGINVTSIAVRNASTSTSVINVTGSPVTTFLAAS
THDDKSLDEAIDMRFDC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018133
ORF Size 1731 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018133.4
RefSeq Size 4696 bp
RefSeq ORF 1734 bp
Locus ID 55167
UniProt ID Q9HCI7
Cytogenetics 3q22.3
Protein Families Druggable Genome
MW 62.5 kDa
Summary Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at lysine 16 which is implicated in the formation of higher-order chromatin structure. Acts as an E3 ubiquitin ligase that promotes monoubiquitination of histone H2B at 'Lys-35' (H2BK34Ub), but not that of H2A. This activity is greatly enhanced by heterodimerization with MSL1. H2B ubiquitination in turn stimulates histine H3 methylation at 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) and leads to gene activation, including that of HOXA9 and MEIS1.UniProtKB/Swiss-Prot Function
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"NM_018133" in other vectors (6)
SKU Description Size Price
RC210829L1 Lenti ORF clone of Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1, Myc-DDK-tagged 10 ug
$837.00
RC210829L2 Lenti ORF clone of Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1, mGFP tagged 10 ug
$837.00
RC210829L3 Lenti ORF clone of Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1, Myc-DDK-tagged 10 ug
$837.00
RC210829L4 Lenti ORF clone of Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1, mGFP tagged 10 ug
$837.00
RG210829 MSL2 (tGFP-tagged) - Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1 10 ug
$489.00 $737.00
SC127078 MSL2 (untagged)-Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1 10 ug
$538.00

Plasmid Map for RC210829

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210829
Circular map for RC210829

file-alt Citations

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