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MSL2L1 (MSL2) (NM_018133) Human Untagged Clone

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SKU
SC127078
MSL2 (untagged)-Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1
  $538.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol MSL2L1
Synonyms MSL-2; MSL2L1; RNF184
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC127078 sequence for NM_018133 edited (data generated by NextGen Sequencing)
ATGAACCCCGTGAATGCTACTGCTCTCTACATTTCCGCGAGCCGCCTAGTGCTCAACTAC
GACCCCGGAGACCCCAAGGCGTTTACTGAGATTAACAGGCTCTTGCCTTACTTCCGACAG
TCCCTTTCGTGCTGTGTTTGCGGACATTTGCTACAAGATCCTATTGCACCCACCAACTCC
ACCTGCCAACATTATGTCTGCAAAACTTGTAAAGGCAAGAAAATGATGATGAAACCTTCC
TGTAGCTGGTGCAAAGACTATGAGCAGTTTGAGGAAAACAAGCAGTTAAGCATCCTAGTG
AACTGCTACAAAAAACTATGCGAGTATATAACACAGACTACACTGGCACGGGATATAATA
GAAGCAGTTGACTGTTCTTCTGATATTTTGGCTTTGCTTAATGATGGATCATTGTTTTGT
GAGGAGACAGAAAAACCCTCAGATTCATCCTTTACTTTGTGTTTGACACATTCCCCTTTA
CCTTCAACCTCAGAACCCACAACTGATCCTCAAGCTAGTTTATCTCCAATGTCTGAAAGC
ACCCTCAGCATTGCTATTGGCAGTTCTGTTATCAATGGTTTGCCTACTTATAATGGGCTT
TCAATAGATAGATTTGGTATAAATATTCCTTCACCTGAACATTCAAATACGATTGACGTA
TGTAATACTGTTGACATAAAAACTGAGGATCTGTCTGACAGCCTGCCACCCGTTTGTGAC
ACAGTAGCCACTGACTTATGTTCCACAGGCATTGATATCTGCAGTTTCAGTGAAGATATA
AAACCTGGAGACTCTCTGTTACTGAGTGTTGAGGAAGTACTCCGCAGCTTAGAAACTGTT
TCAAATACAGAGGTCTGTTGCCCTAATTTGCAGCCGAACTTGGAAGCCACTGTATCCAAT
GGACCTTTTCTGCAGCTTTCTTCCCAGTCTCTTAGCCATAATGTTTTTATGTCCACCAGT
CCTGCACTTCATGGGTTATCATGTACAGCAGCAACTCCGAAGATAGCAAAATTGAATAGA
AAACGATCCAGATCAGAGAGTGACAGTGAGAAAGTTCAGCCACTTCCAATTTCTACCATT
ATCCGAGGCCCAACACTGGGGGCATCTGCTCCTGTGACAGTGAAACGGGAGAGCAAAATT
TCTCTTCAACCTATAGCAACTGTTCCCAATGGAGGCACAACACCTAAAATCAGCAAAACT
GTACTTTTATCTACTAAAAGCATGAAAAAGAGTCATGAACATGGATCCAAGAAATCTCAC
TCTAAAACCAAGCCAGGTATTCTTAAAAAAGACAAAGCAGTAAAGGAAAAGATTCCTAGT
CATCATTTTATGCCAGGAAGTCCTACCAAGACTGTGTACAAAAAACCCCAGGAAAAGAAA
GGGTGTAAATGTGGGCGTGCTACTCAAAATCCAAGTGTTCTTACATGCCGAGGCCAACGC
TGCCCTTGCTACTCTAACCGCAAAGCCTGCTTAGATTGTATATGTCGTGGCTGCCAAAAC
TCCTATATGGCCAATGGGGAGAAGAAGCTGGAGGCATTTGCCGTGCCAGAAAAGGCCTTG
GAGCAGACCAGGCTCACTTTGGGCATTAACGTGACTAGCATTGCTGTGCGTAACGCTAGT
ACCAGCACCAGTGTAATAAATGTCACAGGGTCCCCAGTAACGACGTTTTTAGCTGCCAGT
ACACATGATGATAAAAGTTTGGATGAAGCTATAGACATGAGATTCGACTGTTAA

Clone variation with respect to NM_018133.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_018133 unedited
GGGGTTCACAATTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCAAACTT
GCACGGATTTTGGTTACTTGTTTGGCCAATGGTTCGGCTCTGATTTCTTCCGAATTGTTG
CAAATTCGCCATCGTTCCCTGGCTGCTTACTAAGTTGGATCCGGAATTTCTTTTCAACCG
GGAGCCATTGGTGTCGAAGTGTCTGCAATGAACCCCGTGAATGCTACTGCTCTCTACATT
TCCGCGAGCCGCCTAGTGCTCAACTACGACCCCGGAGACCCCAAGGCGTTTACTGAGATT
AACAGGCTCTTGCCTTACTTCCGACAGTCCCTTTCGTGCTGTGTTTGCGGACATTTGCTA
CAAGATCCTATTGCACCCACCAACTCCACCTGCCAACATTATGTCTGCAAAACTTGTAAA
GGCAAGAAAATGATGATGAAACCTTCCTGTAGCTGGTGCAAAGACTATGAGCAGTTTGAG
GAAAACAAGCAGTTAAGCATCCTAGTGAACTGCTACAAAAAACTATGCGAGTATATAACA
CAGACTACACTGGCACGGGATATAATAGAAGCAGTTGACTGTTCTTCTGATATTTTGGCT
TTGCTTAATGATGGATCATTGTTTTGTGAGGAGACAGAAAAACCCTCAGATTCATCCTTT
ACTTTGTGTTTGACACATTCCCCTTTACCTTCAACCTCAGAACCCACAACTGATCCTCAA
GCTAGTTTATCTCCAATGTCTGAAAGCACCCTCAGCATTGCTATTGGCAGTTCTGTTATC
AATGGTTTGCCTACTTATAATGGGCTNTCAATAGATAGATTTGGTATAAATATTCCTTCA
CCTGAACATTCAAATACGATTGACGTATGTAATACTGTTGACATAAAAACTNGAGATCTG
TCTGACAGCCTGNCACCCCGTTGT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_018133 unedited
NGGGTATTCTATGNACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTTTTCA
TTTTCCACCATTTACTCTTGTTATCAAAAAAGTTCAACTCTTCTTGCAAAACAAAAACAA
AATAGTACATACTGGACACATACATCACATTTTTCTTCCTATGGCTTTAGCCCACCCCCA
CCCCACCAAAAGAGACCAAAAAAAAAAAAAGAAAAAAAAGCCCAACAACAACAACAAAAA
CAACTCTACCTGACCACATTCACAGAAAATGACACCAGGATACACTACAAAACAGAAGGA
GGTGTCATCTGCTCTGTGTCCAAAGGTTTCTTCTCCATGTCTTGTACTAGGCGAGTAACC
ATCATTGAACATGCTGTGTGCCAAATCAAAACATAACTTCAGCATATGTCAGATCTTACT
AGAGATGGTGAACGTAGTAGAAATTGGAAATTTTCCAGCAGTATTTTTCTTTAAATAAGC
ACTGTCAAAGCTGCAGCTCTTCTTTTAAATCACAGGTTATTTCATTACACCTAGTCAGTC
CTTGTTTTATTTGGGCTGTGCTCTTTCAAGCAACTGACTAGATTTCCCTTCAACAGAATG
TTTGTGACTCACTTGTCTTCCCCATAAAAATTAACCGGAAGAAGTGCATTTAAACTGAAA
CATACTTTGTGCTTTACATGCAAAAGAGCATTCTAAAGAAAATCCTCCTAGCTTCAACCT
ACCTAAATGCACAGGCTTTATAAAAAGGCATTNCTATTAGGCCTCTATAAAGAAGCCTTC
ATATGAACAGCACCCTAAAAAAACTAGGAGCATATTCAAAATAAGACTGTGGCANATAAC
TATTAGAGAAATAGTCAAGTGGCGGGCAGTGGGCATAGTACATACACAATCTGGGCAGTC
TGGAGCATT
Restriction Sites NotI-NotI |
ACCN NM_018133
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018133.2, NP_060603.2
RefSeq Size 4696 bp
RefSeq ORF 1734 bp
Locus ID 55167
UniProt ID Q9HCI7
Cytogenetics 3q22.3
Protein Families Druggable Genome
Summary Component of histone acetyltransferase complex responsible for the majority of histone H4 acetylation at lysine 16 which is implicated in the formation of higher-order chromatin structure. Acts as an E3 ubiquitin ligase that promotes monoubiquitination of histone H2B at 'Lys-35' (H2BK34Ub), but not that of H2A. This activity is greatly enhanced by heterodimerization with MSL1. H2B ubiquitination in turn stimulates histine H3 methylation at 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) and leads to gene activation, including that of HOXA9 and MEIS1.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longer isoform (1).
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Documents
Resources
"NM_018133" in other vectors (6)
SKU Description Size Price
RC210829 MSL2 (Myc-DDK-tagged)-Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1 10 ug
$537.00
RC210829L1 Lenti ORF clone of Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1, Myc-DDK-tagged 10 ug
$837.00
RC210829L2 Lenti ORF clone of Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1, mGFP tagged 10 ug
$837.00
RC210829L3 Lenti ORF clone of Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1, Myc-DDK-tagged 10 ug
$837.00
RC210829L4 Lenti ORF clone of Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1, mGFP tagged 10 ug
$837.00
RG210829 MSL2 (tGFP-tagged) - Human male-specific lethal 2 homolog (Drosophila) (MSL2), transcript variant 1 10 ug
$489.00 $737.00

file-alt Citations

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