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HSF2 (NM_004506) Human Tagged ORF Clone

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SKU
RC210751
HSF2 (Myc-DDK-tagged)-Human heat shock transcription factor 2 (HSF2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $499.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HSF2
Synonyms HSF 2; HSTF 2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210751 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCAGAGTTCGAACGTGCCGGCTTTCCTCAGCAAGCTGTGGACGCTTGTGGAGGAAACCCACACTA
ACGAGTTCATCACCTGGAGCCAGAATGGCCAAAGTTTTCTGGTCTTGGATGAGCAACGATTTGCAAAAGA
AATTCTTCCCAAATATTTCAAGCACAATAATATGGCAAGCTTTGTGAGGCAACTGAATATGTATGGTTTC
CGTAAAGTAGTACATATCGACTCTGGAATTGTAAAGCAAGAAAGAGATGGTCCTGTAGAATTTCAGCATC
CTTACTTCAAACAAGGACAGGATGACTTGTTGGAGAACATTAAAAGGAAGGTTTCATCTTCAAAACCAGA
AGAAAATAAAATTCGTCAGGAAGATTTAACAAAAATTATAAGTAGTGCTCAGAAGGTTCAGATAAAACAG
GAAACTATTGAGTCCAGGCTTTCTGAATTAAAAAGTGAGAATGAGTCCCTTTGGAAGGAGGTGTCAGAAT
TACGAGCAAAGCATGCACAACAGCAACAAGTTATTCGAAAGATTGTCCAGTTTATTGTTACATTGGTTCA
AAATAACCAACTTGTGAGTTTAAAACGTAAAAGGCCTCTACTTCTAAACACTAATGGAGCCCAAAAGAAG
AACCTGTTTCAGCACATAGTCAAAGAACCAACTGATAATCATCATCATAAAGTTCCACACAGTAGGACTG
AAGGTTTAAAGCCAAGGGAGAGGATTTCAGATGACATCATTATTTATGATGTTACTGATGATAATGCAGA
TGAAGAAAATATCCCAGTTATTCCAGAAACTAATGAGGATGTTATATCTGATCCCTCCAACTGTAGCCAG
TACCCTGATATTGTCATCGTTGAAGATGACAATGAAGATGAGTATGCACCTGTCATTCAGAGTGGAGAGC
AGAATGAACCAGCCAGAGAATCCCTAAGTTCAGGCAGTGATGGCAGCAGCCCTCTCATGTCTAGTGCTGT
CCAGCTAAATGGCTCATCCAGTCTGACCTCAGAAGATCCAGTGACCATGATGGATTCCATTTTGAATGAT
AACATCAATCTTTTGGGAAAGGTTGAGCTGTTGGATTATCTTGACAGTATTGACTGCAGTTTAGAGGACT
TCCAGGCCATGCTATCAGGAAGACAATTTAGCATAGACCCAGATCTCCTGGTTGATCTTTTCACTAGTTC
TGTGCAGATGAATCCCACAGATTACATCAATAATACAAAATCTGAGAATAAAGGATTAGAAACTACCAAG
AACAATGTAGTTCAGCCAGTTTCGGAAGAGGGAAGAAAATCTAAATCCAAACCAGATAAGCAGCTTATCC
AGTATACCGCCTTTCCACTTCTTGCATTCCTCGATGGGAACCCTGCTTCTTCTGTTGAACAGGCGAGTAC
AACAGCATCATCAGAAGTTTTGTCCTCTGTAGATAAACCCATAGAAGTTGATGAGCTTCTGGATAGCAGC
CTAGACCCAGAACCAACCCAAAGTAAGCTTGTTCGCCTGGAGCCATTGACTGAAGCTGAAGCTAGTGAAG
CTACACTGTTTTATTTATGTGAACTTGCTCCTGCACCTCTGGATAGTGATATGCCACTTTTAGATAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210751 protein sequence
Red=Cloning site Green=Tags(s)

MKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGF
RKVVHIDSGIVKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQ
ETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAQKK
NLFQHIVKEPTDNHHHKVPHSRTEGLKPRERISDDIIIYDVTDDNADEENIPVIPETNEDVISDPSNCSQ
YPDIVIVEDDNEDEYAPVIQSGEQNEPARESLSSGSDGSSPLMSSAVQLNGSSSLTSEDPVTMMDSILND
NINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDLFTSSVQMNPTDYINNTKSENKGLETTK
NNVVQPVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNPASSVEQASTTASSEVLSSVDKPIEVDELLDSS
LDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004506
ORF Size 1608 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004506.4
RefSeq Size 2697 bp
RefSeq ORF 1611 bp
Locus ID 3298
UniProt ID Q03933
Cytogenetics 6q22.31
Domains HSF
Protein Families Transcription Factors
MW 60.3 kDa
Summary The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2011
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Documents
Resources
"NM_004506" in other vectors (6)
SKU Description Size Price
RC210751L1 Lenti ORF clone of Human heat shock transcription factor 2 (HSF2), transcript variant 1, Myc-DDK-tagged 10 ug
$799.00
RC210751L2 Lenti ORF clone of Human heat shock transcription factor 2 (HSF2), transcript variant 1, mGFP tagged 10 ug
$799.00
RC210751L3 Lenti ORF clone of Human heat shock transcription factor 2 (HSF2), transcript variant 1, Myc-DDK-tagged 10 ug
$799.00
RC210751L4 Lenti ORF clone of Human heat shock transcription factor 2 (HSF2), transcript variant 1, mGFP tagged 10 ug
$799.00
RG210751 HSF2 (tGFP-tagged) - Human heat shock transcription factor 2 (HSF2), transcript variant 1 10 ug
$489.00 $699.00
SC125521 HSF2 (untagged)-Human heat shock transcription factor 2 (HSF2), transcript variant 1 10 ug
$500.00

Plasmid Map for RC210751

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210751
Circular map for RC210751

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