TTC8 (NM_198309) Human Tagged ORF Clone

SKU RC210638

TTC8 (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 8 (TTC8), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TTC8
• Synonyms: BBS8; RP51
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210638 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCTCGGAGATGGAGCCGCTGCTCCTGGCCTGGAGCTATTTTAGGCGCAGGAAGTTCCAGCTCTGCG; CCGATCTATGCACGCAGATGCTGGAGAAGTCCCCTTATGACCAGGCAGCTTGGATCTTAAAAGCAAGAGC; GCTAACAGAAATGGTATACATAGATGAAATTGATGTAGATCAGGAAGGAATTGCAGAAATGATGCTGGAT; GAAAATGCTATAGCTCAAGTTCCACGCCCTGGAACGTCTTTGAAACTCCCTGGAACTAATCAGACAGGAG; GGCCTAGCCAGGCCGTTAGGCCAATCACACAAGCTGGAAGACCCATTACAGGTTTCCTCAGGCCCAGCAC; GCAGAGTGGAAGGCCAGGCACTATGGAACAGGCTATCAGAACACCCAGAACCGCCTACACAGCCCGCCCT; ATCACCAGCTCCTCCGGAAGATTTGTCAGGCTGGGAACGGCTTCCATGCTTACAAGTCCTGATGGACCAT; TTATAAATTTATCTAGGCTGAATTTAACAAAGTATTCCCAGAAACCTAAGTTGGCAAAGGCTTTGTTTGA; GTATATCTTTCATCATGAAAATGATGTTAAGACTGCTTTGGATCTGGCTGCCCTCTCCACAGAACATTCT; CAGTACAAGGACTGGTGGTGGAAAGTACAGATTGGAAAATGTTACTACAGGTTGGGAATGTATCGTGAAG; CAGAAAAACAGTTTAAATCAGCCCTGAAGCAGCAGGAAATGGTAGATACATTTCTGTACTTGGCAAAAGT; TTATGTCTCATTGGATCAACCTGTGACTGCTTTAAATCTTTTCAAACAAGGCTTAGATAAGTTTCCAGGA; GAAGTAACCCTGCTCTGTGGAATTGCAAGAATCTATGAGGAAATGAACAATATGTCATCAGCAGCAGAAT; ATTACAAAGAAGTTTTGAAACAAGACAATACTCATGTGGAAGCCATCGCATGCATTGGAAGCAACCACTT; CTATTCTGATCAGCCAGAAATAGCTCTCCGGTTTTACAGGCGGCTGCTGCAGATGGGCATTTATAACGGC; CAGCTTTTTAACAATCTGGGGCTGTGTTGCTTCTATGCCCAGCAGTATGATATGACTCTGACCTCATTTG; AACGTGCCCTTTCTTTGGCTGAAAATGAAGAAGAGGCAGCTGATGTCTGGTACAACTTGGGACATGTAGC; TGTGGGAATAGGAGATACAAATTTGGCCCATCAGTGCTTCAGGCTGGCTCTGGTCAACAACAACAACCAC; GCCGAGGCCTACAACAACCTGGCTGTGCTGGAGATGCGGAAGGGCCACGTTGAACAGGCAAGGGCACTAT; TACAAACTGCATCATCATTAGCACCCCATATGTATGAACCGCATTTTAATTTTGCAACAATCTCTGATAA; GATTGGAGATCTGCAGAGAAGCTATGTTGCTGCGCAGAAGTCTGAAGCAGCATTTCCAGACCATGTGGAC; ACACAACATTTAATTAAACAATTAAGGCAGCATTTTGCTATGCTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210638 protein sequence; Red=Cloning site Green=Tags(s); ; MSSEMEPLLLAWSYFRRRKFQLCADLCTQMLEKSPYDQAAWILKARALTEMVYIDEIDVDQEGIAEMMLD; ENAIAQVPRPGTSLKLPGTNQTGGPSQAVRPITQAGRPITGFLRPSTQSGRPGTMEQAIRTPRTAYTARP; ITSSSGRFVRLGTASMLTSPDGPFINLSRLNLTKYSQKPKLAKALFEYIFHHENDVKTALDLAALSTEHS; QYKDWWWKVQIGKCYYRLGMYREAEKQFKSALKQQEMVDTFLYLAKVYVSLDQPVTALNLFKQGLDKFPG; EVTLLCGIARIYEEMNNMSSAAEYYKEVLKQDNTHVEAIACIGSNHFYSDQPEIALRFYRRLLQMGIYNG; QLFNNLGLCCFYAQQYDMTLTSFERALSLAENEEEAADVWYNLGHVAVGIGDTNLAHQCFRLALVNNNNH; AEAYNNLAVLEMRKGHVEQARALLQTASSLAPHMYEPHFNFATISDKIGDLQRSYVAAQKSEAAFPDHVD; TQHLIKQLRQHFAML; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198309
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_198309.3
• RefSeq Size: 2317 bp
• RefSeq ORF: 1518 bp
• Locus ID: 123016
• UniProt ID: Q8TAM2
• Cytogenetics: 14q31.3
• MW: 57.3 kDa
• Summary: This gene encodes a protein that has been directly linked to Bardet-Biedl syndrome. The primary features of this syndrome include retinal dystrophy, obesity, polydactyly, renal abnormalities and learning disabilities. Experimentation in non-human eukaryotes suggests that this gene is expressed in ciliated cells and that it is involved in the formation of cilia. A mutation in this gene has also been implicated in nonsyndromic retinitis pigmentosa. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]