PLRG1 (NM_002669) Human Tagged ORF Clone

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SKU
RC210603
PLRG1 (Myc-DDK-tagged)-Human pleiotropic regulator 1 (PLRG1), transcript variant 1
  $479.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PLRG1
Synonyms Cwc1; PRL1; PRP46; PRPF46; TANGO4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210603 representing NM_002669
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCGAGGAGGTACAGAAACATTCTGTACACACCCTTGTGTTCAGGTCGTTGAAGAGGACCCATGACA
TGTTTGTAGCTGATAATGGAAAACCTGTGCCTTTAGATGAAGAGAGTCACAAACGAAAAATGGCAATCAA
GCTTCGTAATGAGTATGGTCCTGTGTTGCATATGCCTACTTCAAAAGAAAATCTTAAAGAGAAGGGTCCT
CAGAATGCAACGGATTCATATGTTCATAAACAGTACCCTGCCAATCAAGGACAAGAAGTTGAATACTTTG
TGGCAGGTACACATCCATACCCACCAGGACCTGGGGTTGCTTTGACAGCAGATACTAAGATCCAGAGAAT
GCCAAGTGAATCAGCTGCACAGTCCTTAGCGGTGGCATTACCTTTGCAGACCAAGGCTGATGCAAATCGT
ACTGCCCCTAGTGGAAGTGAATACCGACATCCTGGGGCTTCTGACCGTCCACAGCCTACAGCGATGAATT
CAATTGTCATGGAGACTGGCAATACCAAGAACTCTGCACTGATGGCTAAAAAAGCCCCTACAATGCCAAA
ACCCCAGTGGCACCCACCGTGGAAACTCTACAGGGTTATCAGTGGGCATCTTGGCTGGGTTCGATGTATT
GCTGTGGAACCTGGAAATCAGTGGTTTGTTACTGGATCTGCTGACAGAACTATAAAGATCTGGGACTTGG
CTAGTGGCAAATTAAAACTGTCATTGACTGGGCATATTAGTACTGTGCGGGGCGTGATAGTAAGCACAAG
GAGCCCATATCTGTTCTCTTGTGGAGAAGACAAACAAGTGAAATGCTGGGATCTCGAATACAATAAGGTT
ATACGGCATTATCATGGACATTTAAGTGCAGTGTATGGTTTGGATTTGCACCCGACAATCGATGTGTTGG
TAACCTGTAGTCGAGATTCAACTGCACGGATTTGGGATGTGAGAACTAAAGCCAGTGTACACACATTATC
TGGACATACAAATGCAGTTGCTACAGTGAGATGTCAGGCTGCAGAACCACAAATTATTACAGGAAGCCAT
GATACTACAATTCGATTATGGGATCTGGTGGCTGGAAAAACAAGAGTGACATTAACAAATCACAAAAAAT
CAGTTAGGGCTGTGGTTTTACATCCAAGACATTACACATTTGCATCTGGTTCTCCAGATAACATAAAGCA
GTGGAAATTCCCTGATGGAAGTTTCATTCAAAATCTTTCCGGTCATAATGCTATTATTAACACATTGACG
GTAAATTCTGATGGAGTGCTTGTATCTGGAGCTGACAATGGCACCATGCATCTTTGGGACTGGAGAACTG
GCTACAATTTTCAGAGAGTTCACGCAGCTGTGCAACCTGGGTCTTTGGACAGTGAATCAGGAATATTTGC
TTGTGCTTTTGATCAGTCTGAAAGTCGATTACTAACAGCTGAAGCTGATAAAACCATTAAAGTATACAGA
GAGGATGACACAGCCACAGAAGAAACTCATCCAGTCAGCTGGAAACCAGAAATTATCAAGAGAAAGAGAT
TT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210603 representing NM_002669
Red=Cloning site Green=Tags(s)

MVEEVQKHSVHTLVFRSLKRTHDMFVADNGKPVPLDEESHKRKMAIKLRNEYGPVLHMPTSKENLKEKGP
QNATDSYVHKQYPANQGQEVEYFVAGTHPYPPGPGVALTADTKIQRMPSESAAQSLAVALPLQTKADANR
TAPSGSEYRHPGASDRPQPTAMNSIVMETGNTKNSALMAKKAPTMPKPQWHPPWKLYRVISGHLGWVRCI
AVEPGNQWFVTGSADRTIKIWDLASGKLKLSLTGHISTVRGVIVSTRSPYLFSCGEDKQVKCWDLEYNKV
IRHYHGHLSAVYGLDLHPTIDVLVTCSRDSTARIWDVRTKASVHTLSGHTNAVATVRCQAAEPQIITGSH
DTTIRLWDLVAGKTRVTLTNHKKSVRAVVLHPRHYTFASGSPDNIKQWKFPDGSFIQNLSGHNAIINTLT
VNSDGVLVSGADNGTMHLWDWRTGYNFQRVHAAVQPGSLDSESGIFACAFDQSESRLLTAEADKTIKVYR
EDDTATEETHPVSWKPEIIKRKRF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002669
ORF Size 1542 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002669.4
RefSeq Size 3374 bp
RefSeq ORF 1545 bp
Locus ID 5356
UniProt ID O43660
Cytogenetics 4q31.3
Domains WD40
Protein Families Transcription Factors
Protein Pathways Spliceosome
MW 57.2 kDa
Summary This gene encodes a core component of the cell division cycle 5-like (CDC5L) complex. The CDC5L complex is part of the spliceosome and is required for pre-mRNA splicing. The encoded protein plays a critical role in alternative splice site selection. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Jan 2011
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Documents
Resources
"NM_002669" in other vectors (4)
SKU Description Size Price
RC210603L3 Lenti-ORF clone of PLRG1 (Myc-DDK-tagged)-Human pleiotropic regulator 1 (PLRG1), transcript variant 1 10 ug
$779.00
RC210603L4 Lenti-ORF clone of PLRG1 (mGFP-tagged)-Human pleiotropic regulator 1 (PLRG1), transcript variant 1 10 ug
$779.00
RG210603 PLRG1 (tGFP-tagged) - Human pleiotropic regulator 1 (PLRG1), transcript variant 1 10 ug
$679.00
SC118503 PLRG1 (untagged)-Human pleiotropic regulator 1 (PLRG1), transcript variant 1 10 ug
$479.00

Plasmid Map for RC210603

Download sequence for

Cloning scheme for RC210603

Circular map for RC210603

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