CREB1 (NM_004379) Human Tagged ORF Clone
SKU
RC210577
CREB1 (Myc-DDK-tagged)-Human cAMP responsive element binding protein 1 (CREB1), transcript variant A
$289.00
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CREB1 |
| Synonyms | CREB; CREB-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004379 |
| ORF Size | 981 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004379.5 |
| RefSeq Size | 9752 bp |
| RefSeq ORF | 984 bp |
| Locus ID | 1385 |
| UniProt ID | P16220 |
| Cytogenetics | 2q33.3 |
| Domains | BRLZ, pKID |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Antigen processing and presentation, Huntington's disease, Melanogenesis, Prostate cancer |
| MW | 35.1 kDa |
| Summary | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. provided by RefSeq, Mar 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004379" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210577L1 | Lenti ORF clone of Human cAMP responsive element binding protein 1 (CREB1), transcript variant A, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC210577L2 | Lenti ORF clone of Human cAMP responsive element binding protein 1 (CREB1), transcript variant A, mGFP tagged | 10 ug |
$600.00
|
|
| RC210577L3 | Lenti ORF clone of Human cAMP responsive element binding protein 1 (CREB1), transcript variant A, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC210577L4 | Lenti ORF clone of Human cAMP responsive element binding protein 1 (CREB1), transcript variant A, mGFP tagged | 10 ug |
$600.00
|
|
| RG210577 | CREB1 (tGFP-tagged) - Human cAMP responsive element binding protein 1 (CREB1), transcript variant A | 10 ug |
$489.00
$500.00
|
|
| SC117388 | CREB1 (untagged)-Human cAMP responsive element binding protein 1 (CREB1), transcript variant A | 10 ug |
$300.00
|
|
Citations
Novel live cell fluorescent probe for human-induced pluripotent stem cells highlights early reprogramming population
Sriram, Kang, Subramanian et al
Stem Cell Res Ther (2021) 12 (1), 113 DOI: 10.1186/s13287-021-02171-6
Sriram, Kang, Subramanian et al
Stem Cell Res Ther (2021) 12 (1), 113 DOI: 10.1186/s13287-021-02171-6
Cited by 5
AI Summary: OriGene’s CREB1 Human cDNA ORF clone was used in this study to overexpress CREB1 during reprogramming, enabling the authors to assess its role in enhancing the efficiency of human iPS cell generation.
Snippet: malized to the level of GAPDH. CREB1 overexpression and silencing by siRNA CREB1 was overexpressed in ASCs and DPSCs during reprogramming using the commercially available CREB1 Human cDNA ORF clone (Origene) according to manufacturer’s instructions. Knockdown of CREB1 was achieved using the ON-TARGETplus Human CREB1 siRNA – SmartPool (GE Dharmacon) according to manufacturer’s instructions
The endoplasmic reticulum acetyltransferases ATase1/NAT8B and ATase2/NAT8 are differentially regulated to adjust engagement of the secretory pathway
Rigby, Ding, Farrugia et al
J. Neurochem. (2020)
Rigby, Ding, Farrugia et al
J. Neurochem. (2020)
Cited by 16
AI Summary: OriGene products—including the anti-DDK antibody (TA50011), pCMV6-entry plasmid backbone (PS100001), and expression plasmids for human NAT8B (RC215647), NAT8 (RC203157), CREB1 (RC210577), c-FOS (RC202597), c-JUN (RC209804), and p53 (RC200003)—were used for protein detection and gene overexpression experiments that enabled the study of regulatory mechanisms controlling ATase1/NAT8B and ATase2/NAT8 in the secretory pathway.
Snippet: lysine (Abcam #ab409;RRID:AB_308707), anti-DDK (Origene TA50011;RRID:AB_2622345), and anti-Myc (Santa Cruz Biotechnology sc-40;RRID:AB_627268) (pCMV6-entry backbone;OriGene #PS100001;2017) or empty plasmid (serving as a control). … pCMV6-entry empty plasmid (OriGene, #PS100001;2017);cells were harvested 48 hr after transfection. PC12 stable lines were generated by transfecting with Lipofectamine 2000 using the following plasmids: pCMV6-entry empty plasmid (described above), human NAT8B-pCMV6 (OriGene, #RC215647;2018), or human NAT8-pCMV6 (OriGene, #RC203157;2018)...DDK (Origene, TA50011;RRID:AB_2622345)...transcription factor expression plasmid (pCMV6-entry backbone;OriGene, #PS100001;2017)...human CREB1-pCMV6 (Origene, #RC210577;2017), human c-FOS-pCMV6 (Origene, #RC202597;2017), human c-JUN-pCMV6 (Origene, #RC209804;2017), p53-pCMV6 (OriGene, #RC200003;2019)
FOXQ1 controls the induced differentiation of melanocytic cells
Bagati, Bianchi-Smiraglia, Moparthy et al
Cell Death Differ. (2018) 25 (6), 1040-1049 DOI: 10.1038/s41418-018-0066-y
Bagati, Bianchi-Smiraglia, Moparthy et al
Cell Death Differ. (2018) 25 (6), 1040-1049 DOI: 10.1038/s41418-018-0066-y
Downregulation of microRNA-27b-3p enhances tamoxifen resistance in breast cancer by increasing NR5A2 and CREB1 expression
Zhu, Zou, Nie et al
Cell Death Dis (2016) 7 (11), e2454 DOI: 10.1038/cddis.2016.361
Zhu, Zou, Nie et al
Cell Death Dis (2016) 7 (11), e2454 DOI: 10.1038/cddis.2016.361
Cited by 104
CREB Negatively Regulates IGF2R Gene Expression and Downstream Pathways to Inhibit Hypoxia-Induced H9c2 Cardiomyoblast Cell Death
Chen, Kuo, Hsieh et al
Int J Mol Sci (2015) 16 (11), 27921-30 DOI: 10.3390/ijms161126067
Chen, Kuo, Hsieh et al
Int J Mol Sci (2015) 16 (11), 27921-30 DOI: 10.3390/ijms161126067
Cited by 21
AI Summary: OriGene's CREB plasmids were used to overexpress CREB in H9c2 cardiomyoblasts, enabling the study to demonstrate CREB's role in downregulating IGF2R expression and protecting cells from hypoxia-induced apoptosis.
Snippet: CREB plasmids were procured from OriGene (Level Biotechnology Inc., Taipei, Taiwan), and H9c2 cells were transfected for using PureFection reagents (System Biosciences, Mountain View, CA, USA) for 24 h. 4.2. Western Blot. ... Cite SaveSaving
The cAMP responsive element binding protein 1 transactivates epithelial membrane protein 2, a potential tumor suppressor in the urinary bladder urothelial carcinoma
Li, Wu, Wu et al
Oncotarget (2015) 6 (11), 9220-39 DOI: 10.18632/oncotarget.3312
Li, Wu, Wu et al
Oncotarget (2015) 6 (11), 9220-39 DOI: 10.18632/oncotarget.3312
Cited by 34
AI Summary: OriGene products contributed to this study by providing the CREB1 expression plasmid (RC209451) and EMP2 shRNA (TL315973), which were used for gene overexpression and knockdown experiments to investigate the regulatory relationship between CREB1 and EMP2 in bladder cancer cells.
Showing 1-6 of 7 papers.
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