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ARFRP1 (NM_003224) Human Tagged ORF Clone

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SKU
RC210518
ARFRP1 (Myc-DDK-tagged)-Human ADP-ribosylation factor related protein 1 (ARFRP1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "ARFRP1 or NM_003224"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ARFRP1
Synonyms ARL18; ARP; Arp1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210518 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACACGCTGCTGTCGGGCTTGTACAAGTACATGTTTCAGAAGGACGAGTACTGCATCCTGATCCTGG
GCCTGGACAATGCTGGGAAGACGACCTTCCTGGAGCAGTCGAAAACCCGATTTAACAAGAACTACAAGGG
GATGAGTCTATCCAAAATCACCACCACCGTGGGCCTAAACATCGGCACTGTGGATGTGGGAAAGGCTCGG
CTCATGTTCTGGGACTTAGGAGGGCAGGAAGAGCTGCAGTCTTTGTGGGACAAGTATTATGCGGAGTGTC
ACGGCGTCATCTACGTCATTGACTCCACCGACGAGGAGAGGCTGGCTGAGTCCAAGCAGGCGTTTGAGAA
GGTGGTGACCAGCGAGGCGCTGTGCGGTGTCCCCGTCTTGGTGCTGGCCAACAAGCAGGATGTGGAGACG
TGCCTCTCAATCCCTGACATCAAGACGGCCTTCAGCGACTGCACCAGCAAGATCGGCAGGCGAGATTGCC
TGACCCAGGCCTGCTCGGCCCTCACAGGCAAAGGGGTGCGCGAGGGCATCGAGTGGATGGTGAAGTGTGT
CGTGCGGAATGTGCACCGGCCGCCGCGGCAGAGGGACATCACG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210518 protein sequence
Red=Cloning site Green=Tags(s)

MYTLLSGLYKYMFQKDEYCILILGLDNAGKTTFLEQSKTRFNKNYKGMSLSKITTTVGLNIGTVDVGKAR
LMFWDLGGQEELQSLWDKYYAECHGVIYVIDSTDEERLAESKQAFEKVVTSEALCGVPVLVLANKQDVET
CLSIPDIKTAFSDCTSKIGRRDCLTQACSALTGKGVREGIEWMVKCVVRNVHRPPRQRDIT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003224
ORF Size 603 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003224.6
RefSeq Size 2571 bp
RefSeq ORF 606 bp
Locus ID 10139
UniProt ID Q13795
Cytogenetics 20q13.33
Domains arf, ARF, RAB, SAR
MW 22.6 kDa
Summary The protein encoded by this gene is a membrane-associated GTP-ase which localizes to the plasma membrane and is related to the ADP-ribosylation factor (ARF) and ARF-like (ARL) proteins. This gene plays a role in membrane trafficking between the trans-Golgi network and endosomes. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, May 2012
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"NM_003224" in other vectors (4)
SKU Description Size Price
RC210518L3 Lenti ORF clone of Human ADP-ribosylation factor related protein 1 (ARFRP1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC210518L4 Lenti ORF clone of Human ADP-ribosylation factor related protein 1 (ARFRP1), transcript variant 1, mGFP tagged 10 ug
$600.00
RG210518 ARFRP1 (tGFP-tagged) - Human ADP-ribosylation factor related protein 1 (ARFRP1), transcript variant 1 10 ug
$489.00 $500.00
SC118098 ARFRP1 (untagged)-Human ADP-ribosylation factor related protein 1 (ARFRP1), transcript variant 1 10 ug
$300.00

Plasmid Map for RC210518

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210518
Circular map for RC210518

file-alt Citations

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