PSMD1 (NM_002807) Human Tagged ORF Clone
SKU
RC210486
PSMD1 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1
$872.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PSMD1 |
| Synonyms | P112; Rpn2; S1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_002807 |
| ORF Size | 2859 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002807.4 |
| RefSeq Size | 3329 bp |
| RefSeq ORF | 2862 bp |
| Locus ID | 5707 |
| UniProt ID | Q99460 |
| Cytogenetics | 2q37.1 |
| Domains | PC_rep |
| Protein Pathways | Proteasome |
| MW | 105.8 kDa |
| Summary | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes the largest non-ATPase subunit of the 19S regulator lid, which is responsible for substrate recognition and binding. There is evidence that this proteasome and its subunits interact with viral proteins, including those of coronaviruses. Alternatively spliced transcript variants have been found for this gene.provided by RefSeq, Aug 2020 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002807" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210486L1 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,172.00
|
|
| RC210486L2 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1, mGFP tagged | 10 ug |
$1,172.00
|
|
| RC210486L3 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,172.00
|
|
| RC210486L4 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1, mGFP tagged | 10 ug |
$1,172.00
|
|
| RG210486 | PSMD1 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1 | 10 ug |
$789.00
$1,072.00
|
|
| SC118428 | PSMD1 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 (PSMD1), transcript variant 1 | 10 ug |
$873.00
|
|
Citations
The autism-linked UBE3A T485A mutant E3 ubiquitin ligase activates the Wnt/β-catenin pathway by inhibiting the proteasome
Yi, Paranjape, Walker et al
J. Biol. Chem. (2017) 292 (30), 12503-12515 DOI: 10.1074/jbc.M117.788448
Yi, Paranjape, Walker et al
J. Biol. Chem. (2017) 292 (30), 12503-12515 DOI: 10.1074/jbc.M117.788448
AI Summary: OriGene products, specifically the pCMV6 Myc-DDK-tagged constructs for PSMB1, PSMC2, PSMC5, PSMC6, PSMD1, PSMD2, PSMD3, PSMD6, PSMD7, PSMD11, and untagged PSMD4, were used to overexpress proteasome subunits and investigate their role in reversing the effects of the autism-linked UBE3A T485A mutant on Wnt/β-catenin signaling.
Snippet: UbG76V-GFP was obtained from Addgene (23969). pCMV6 Myc-DDK-tagged constructs for PSMB1 (RC201798), PSMC2 (RC200945), PSMC5 (RC201251), PSMC6 (RC202809), PSMD1 (RC210486), PSMD2 (RC203204), PSMD3 (RC202307), PSMD6 (RC202292), PSMD7 (RC203133), PSMD11 (RC201201), and untagged PSMD4 (SC111678) were all purchased from Origene
Showing 1-1 of 1 papers.
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