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FBXL7 (NM_012304) Human Tagged ORF Clone

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SKU
RC210441
FBXL7 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 7 (FBXL7)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXL7
Synonyms FBL6; FBL7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210441 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCGCGAACAATGGCAAACAGTACGGCAGTGAGGGCAAAGGCAGCTCGAGCATCTCATCTGACGTGA
GTTCAAGTACAGATCACACGCCCACTAAAGCCCAGAAGAATGTGGCTACCAGCGAAGACTCCGACCTGAG
CATGCGCACACTGAGCACGCCCAGCCCAGCCCTGATATGTCCACCGAATCTCCCAGGATTTCAGAATGGA
AGGGGCTCGTCCACCTCCTCGTCCTCCATCACCGGGGAGACGGTGGCCATGGTGCACTCCCCGCCCCCGA
CCCGCCTCACACACCCGCTCATCCGGCTCGCCTCCAGACCCCAGAAGGAGCAGGCCAGCATAGACCGGCT
CCCGGACCACTCCATGGTGCAGATCTTCTCCTTCCTGCCCACCAACCAGCTGTGCCGCTGCGCGCGAGTG
TGCCGCCGCTGGTACAACCTGGCCTGGGACCCGCGGCTCTGGAGGACTATCCGCCTGACGGGCGAGACCA
TCAACGTGGACCGCGCCCTCAAGGTGCTGACCCGCAGACTCTGCCAGGACACCCCCAACGTGTGTCTCAT
GCTGGAAACCGTAACTGTCAGTGGCTGCAGGCGGCTCACAGACCGAGGGCTGTACACCATCGCCCAGTGC
TGCCCCGAACTGAGGCGACTGGAAGTCTCAGGCTGTTACAATATCTCCAACGAGGCCGTCTTTGATGTGG
TGTCCCTCTGCCCTAATCTGGAGCACCTGGATGTGTCAGGATGCTCCAAAGTGACCTGCATCAGCTTGAC
CCGGGAGGCCTCCATTAAACTGTCACCCTTGCATGGCAAACAGATTTCCATCCGCTACCTGGACATGACG
GACTGCTTCGTGCTGGAGGACGAAGGCCTGCACACCATCGCGGCGCACTGCACGCAGCTCACCCACCTCT
ACCTGCGCCGCTGCGTCCGCCTGACCGACGAAGGCCTGCGCTACCTGGTGATCTACTGCGCCTCCATCAA
GGAGCTGAGCGTCAGCGACTGCCGCTTCGTCAGCGACTTCGGCCTGCGGGAGATCGCCAAGCTGGAGTCC
CGCCTGCGGTACCTGAGCATCGCGCACTGCGGCCGGGTCACCGACGTGGGCATCCGCTACGTGGCCAAGT
ACTGCAGCAAGCTGCGCTACCTCAACGCGAGGGGCTGCGAGGGCATCACGGACCACGGTGTGGAGTACCT
CGCCAAGAACTGCACCAAACTCAAATCCCTGGATATCGGCAAATGCCCTTTGGTATCCGACACGGGCCTG
GAGTGCCTGGCCCTGAACTGCTTCAACCTCAAGCGGCTCAGCCTCAAGTCCTGCGAGAGCATCACCGGCC
AGGGCTTGCAGATCGTGGCCGCCAACTGCTTTGACCTCCAGACGCTGAATGTCCAGGACTGCGAGGTCTC
CGTGGAGGCCCTGCGCTTTGTCAAACGCCACTGCAAGCGCTGCGTCATCGAGCACACCAACCCGGCTTTC
TTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210441 protein sequence
Red=Cloning site Green=Tags(s)

MGANNGKQYGSEGKGSSSISSDVSSSTDHTPTKAQKNVATSEDSDLSMRTLSTPSPALICPPNLPGFQNG
RGSSTSSSSITGETVAMVHSPPPTRLTHPLIRLASRPQKEQASIDRLPDHSMVQIFSFLPTNQLCRCARV
CRRWYNLAWDPRLWRTIRLTGETINVDRALKVLTRRLCQDTPNVCLMLETVTVSGCRRLTDRGLYTIAQC
CPELRRLEVSGCYNISNEAVFDVVSLCPNLEHLDVSGCSKVTCISLTREASIKLSPLHGKQISIRYLDMT
DCFVLEDEGLHTIAAHCTQLTHLYLRRCVRLTDEGLRYLVIYCASIKELSVSDCRFVSDFGLREIAKLES
RLRYLSIAHCGRVTDVGIRYVAKYCSKLRYLNARGCEGITDHGVEYLAKNCTKLKSLDIGKCPLVSDTGL
ECLALNCFNLKRLSLKSCESITGQGLQIVAANCFDLQTLNVQDCEVSVEALRFVKRHCKRCVIEHTNPAF
F

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012304
ORF Size 1473 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012304.5
RefSeq Size 4584 bp
RefSeq ORF 1476 bp
Locus ID 23194
UniProt ID Q9UJT9
Cytogenetics 5p15.1
Domains F-box, LRR, LRR_CC
Protein Families Druggable Genome
MW 54.6 kDa
Summary This gene encodes a member of the F-box protein family which is characterized by a 42-48 amino acid motif, the F-box, which binds to the S-phase kinase-associated protein 1 (Skp1) protein. The F-box proteins constitute one of the four subunits of E3 ubiquitin protein ligases called SCFs (SKP1-Cul1-F-box), which play a role in phosphorylation-dependent ubiquitination of proteins. The F-box proteins are divided into 3 subfamilies based on the other domain in the protein: F-box proteins that also have a WD-40 domain (Fbws subfamily), F-box proteins that also have leucine-rich repeats (Fbls subfamily) and F-box proteins that contain other motifs or lack known protein-interaction domains (Fbxs subfamily). The protein encoded by this gene belongs to the Fbls subfamily. Alternative splicing results in multiple transcript variants of this gene. provided by RefSeq, May 2013
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"NM_012304" in other vectors (6)
SKU Description Size Price
RC210441L1 Lenti ORF clone of Human F-box and leucine-rich repeat protein 7 (FBXL7), Myc-DDK-tagged 10 ug
$757.00
RC210441L2 Lenti ORF clone of Human F-box and leucine-rich repeat protein 7 (FBXL7), mGFP tagged 10 ug
$757.00
RC210441L3 Lenti ORF clone of Human F-box and leucine-rich repeat protein 7 (FBXL7), Myc-DDK-tagged 10 ug
$757.00
RC210441L4 Lenti ORF clone of Human F-box and leucine-rich repeat protein 7 (FBXL7), mGFP tagged 10 ug
$757.00
RG210441 FBXL7 (tGFP-tagged) - Human F-box and leucine-rich repeat protein 7 (FBXL7) 10 ug
$489.00 $657.00
SC115444 FBXL7 (untagged)-Human F-box and leucine-rich repeat protein 7 (FBXL7) 10 ug
$457.00

Plasmid Map for RC210441

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210441
Circular map for RC210441

file-alt Citations

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