Steroid sulfatase (STS) (NM_000351) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC210431L4
Lenti ORF clone of Human steroid sulfatase (microsomal), isozyme S (STS), mGFP tagged
  $843.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Steroid sulfatase
Synonyms ARSC; ARSC1; ASC; ES; SSDD; XLI
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC210431).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000351
ORF Size 1749 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000351.3
RefSeq Size 6377 bp
RefSeq ORF 1737 bp
Locus ID 412
UniProt ID P08842
Cytogenetics Xp22.31
Domains Sulfatase
Protein Families Druggable Genome, Transmembrane
Protein Pathways Androgen and estrogen metabolism
MW 65.5 kDa
Summary This gene encodes a multi-pass membrane protein that is localized to the endoplasmic reticulum. It belongs to the sulfatase family and hydrolyzes several 3-beta-hydroxysteroid sulfates, which serve as metabolic precursors for estrogens, androgens, and cholesterol. Mutations in this gene are associated with X-linked ichthyosis (XLI). Alternatively spliced transcript variants resulting from the use of different promoters have been described for this gene (PMID:17601726). provided by RefSeq, Mar 2016
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"NM_000351" in other vectors (6)
SKU Description Size Price
RC210431 STS (Myc-DDK-tagged)-Human steroid sulfatase (microsomal), isozyme S (STS) 10 ug
$543.00
RC210431L1 Lenti ORF clone of Human steroid sulfatase (microsomal), isozyme S (STS), Myc-DDK-tagged 10 ug
$843.00
RC210431L2 Lenti ORF clone of Human steroid sulfatase (microsomal), isozyme S (STS), mGFP tagged 10 ug
$843.00
RC210431L3 Lenti ORF clone of Human steroid sulfatase (microsomal), isozyme S (STS), Myc-DDK-tagged 10 ug
$843.00
RG210431 STS (tGFP-tagged) - Human steroid sulfatase (microsomal), isozyme S (STS) 10 ug
$489.00 $743.00
SC119923 STS (untagged)-Human steroid sulfatase (microsomal), isozyme S (STS) 10 ug
$544.00

Plasmid Map for RC210431L4

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Cloning scheme for RC210431L4

Circular map for RC210431L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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