Steroid sulfatase (STS) (NM_000351) Human Untagged Clone

SKU SC119923

STS (untagged)-Human steroid sulfatase (microsomal), isozyme S (STS)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Steroid sulfatase
• Synonyms: ARSC; ARSC1; ASC; ES; SSDD; XLI
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000351 edited; ATGCCTTTAAGGAAGATGAAGATCCCTTTCCTCCTACTGTTCTTTCTGTGGGAAGCCGAG; AGCCACGCAGCATCAAGGCCGAACATCATCCTGGTGATGGCTGACGACCTCGGCATTGGA; GATCCTGGGTGCTATGGGAACAAAACTATCAGGACTCCCAATATCGACCGGTTGGCCAGT; GGGGGAGTGAAACTCACTCAGCACCTGGCAGCATCACCGCTGTGCACACCAAGCAGGGCA; GCCTTCATGACTGGCCGGTACCCTGTCCGATCAGGAATGGCATCTTGGTCCCGCACTGGA; GTTTTCCTCTTCACAGCCTCTTCGGGAGGACTTCCCACCGATGAGATTACCTTTGCTAAG; CTTCTGAAGGATCAAGGTTATTCAACAGCACTGATAGGGAAATGGCACCTTGGGATGAGC; TGTCACAGCAAGACTGACTTCTGTCACCACCCTTTACATCACGGCTTCAATTATTTCTAT; GGGATCTCTTTGACCAATCTGAGAGACTGCAAGCCCGGAGAGGGCAGTGTCTTCACCACG; GGCTTCAAGAGGCTGGTCTTCCTCCCCCTGCAGATCGTCGGGGTCACCCTCCTTACCCTT; GCTGCACTCAATTGTCTGGGGCTACTCCACGTGCCTCTAGGCGTTTTTTTCAGCCTTCTC; TTCCTAGCAGCCCTAATCCTGACCCTTTTCTTGGGCTTCCTTCATTACTTCCGGCCCCTG; AACTGCTTCATGATGAGGAACTACGAGATCATTCAGCAGCCCATGTCCTATGACAATCTC; ACCCAGAGGCTAACGGTGGAGGCGGCCCAGTTCATACAGCGGAACACTGAGACTCCGTTC; CTGCTTGTCTTGTCCTACCTCCACGTGCACACAGCCCTGTTCTCCAGCAAAGACTTTGCT; GGCAAAAGTCAACACGGAGTCTACGGGGATGCTGTTGAGGAAATGGACTGGAGTGTGGGG; CAGATCTTGAACCTTCTGGATGAGCTGAGATTGGCTAATGATACCCTCATCTACTTCACA; TCGGACCAGGGAGCACATGTAGAAGAAGTGTCTTCCAAAGGAGAAATTCATGGCGGAAGT; AATGGGATCTATAAAGGAGGAAAAGCAAACAACTGGGAAGGAGGTATCCGGGTTCCAGGC; ATCCTTCGTTGGCCCAGGGTGATACAGGCTGGCCAGAAGATTGATGAGCCCACTAGCAAC; ATGGACATATTTCCTACAGTAGCCAAGCTGGCTGGAGCTCCCTTGCCTGAGGACAGGATC; ATTGATGGACGTGATCTGATGCCCCTGCTTGAAGGAAAAAGCCAACGCTCCGATCATGAG; TTTCTCTTCCATTACTGCAACGCCTACTTAAATGCTGTGCGCTGGCACCCTCAGAACAGC; ACATCCATCTGGAAGGCCTTTTTCTTCACCCCCAACTTCAACCCCGTGGGTTCCAACGGA; TGCTTTGCCACACACGTGTGCTTCTGTTTCGGGAGTTATGTCACCCATCACGACCCACCT; TTACTCTTTGATATTTCCAAAGATCCCAGAGAGAGAAACCCACTAACTCCAGCATCCGAG; CCCCGGTTTTATGAAATCCTCAAAGTCATGCAGGAAGCTGCGGACAGACACACCCAGACC; CTGCCAGAGGTGCCCGATCAGTTTTCATGGAACAACTTTCTTTGGAAGCCCTGGCTTCAG; CTGTGCTGTCCTTCCACCGGCCTGTCTTGCCAGTGTGATAGAGAAAAACAGGATAAGAGA; CTGAGCCGCTAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000351 unedited; TTGCGGATTAGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCTGTAGTGA; GGTTGCAGTGATTGAGTAGGATTGGCCTGCTTCAAAGCAGAGGTTTCTCATGGGAATATG; CTTATTAAACTCCCACTGGTGCAGAAACCATGAACAGAGGATGAACAAGTGAAGTTGCAA; TCTCCTCCATCACAGCTCAGTTCCCCAACAACAGGATCACAAGCTGGAGATGCCTTTAAG; GAAGATGAAGATCCCTTTCCTCCTACTGTTCTTTCTGTGGGAAGCCGAGAGCCACGCAGC; ATCAAGGCCGAACATCATCCTGGTGATGGCTGACGACCTCGGCATTGGAGATCCTGGGTG; CTATGGGAACAAAACTATCAGGACTCCCAATATCGACCGGTTGGCCAGTGGGGGAGTGAA; ACTCACTCAGCACCTGGCAGCATCACCGCTGTGCACACCAAGCAGGGCAGCCTTCATGAC; TGGCCGGTACCCTGTCCGATCAGGAATGGCATCTTGGTCCCGCACTGGAGTTTTCCTCTT; CACAGCCTCTTCGGGAGGACTTCCCACCGATGAGATTACCTTTGCTAAGCTTCTGAAAGA; TCAAGGCTATTCAACAGCACTTGATAGGGAAATGGCACCTTGGGATGAGCTGTCACAGCA; AGACTGACTTCTGTCACCACCCTTTACATTACGGCTTTAATTAATTTCTATGGGATCTCT; TTGACCAATCCTGAAAGACTGCAAGCCCCGAAAAGGCAGTGTCTTCACCACGGGCTTCAA; AAGGCTGGGCTTTCCTCCCCCTGCAATCGTGCGGGGAACCCTTCTTTACCTTGCTGCACC; TCATTGGTCTGGGGCTTCTACACGGGCCTCTTAGGCGCGTTTTTTTCACCCTTCTTTTTC; TCAAAAGCCGTAAACTCGACCCTTTTCTTGGGCTTTCCTCTTTACTTCCGN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000351 unedited; GGCCGCAATTTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTTTTTATGTGATTGCCTTTT; TATTATGCTGGTTATTGCATCACTGCAGATTATAGAGTAAATGGTAACTTATACTGTTGA; CATAGGTATTTGCTGAGGGGTGAGTTAAGGTACCTCCTGCACCTTTCTGTTTTAAGATTG; CCTTCTGCACCTTTTTGTCATCTGCTTGCACTGGTGTGCCTTGTGAATGCCCCTCTATTC; ATTCTATTTCCATGAGTCCAAGACTCTGGCCACAGAAGGCATAAACCTACCATCTTCCTG; TCTGTATACTACAGACCTTATCCCCCTCACCCCTCCAGTTGAGTAGCTGTTGAGCTCTGG; CCCAAGCCTTCAGGTGATAAAATGGAGAATCAGTCCAAATCCAAGGTGTAGATGGAGTTA; TGTTTCCCCAGTGCCACTCTCAGGCGTGTTTGTAGTGAAGATGGTGAGCTTTGCCACATG; CGTCTGTCTGGTCCCCAGGCGCTGCTAGCGGCTCAGTCTCTTATCCTGTTTTTCTCTATC; ACACTGGCAAGACAGGCCCGTGGAAGGACAGCACAGCTGAAGCCAGNGCTTCCAAAGAAA; GTTGTTCCATGAAAACTGATCGGGCACCCTCTGCAGGGTCTGGGTGTGTCTGTCCGCAGC; TTCCTGCATGACTTTGAGGATTTATAAAACCGGNGCTCGGATGCTGAGTTAGGGGGTTTC; TTTCTTGGGACCTTTGGATATCAAAAAGTAAGGTGGTCGTGATGGGTGACTAACTCCCAA; CAGAACCCACGTGGGGGCAAACCTTCCGTGAACCACCGGGTTGAATCGGGGTGAAAAAAA; GCCTTCCAATGATGGCTGTTCTGAGTGGCCCCCCCCCTTTTATAGGCGTGCATTATGGAA; AAAATCCTGATCGACGTTGCTTTTCTTAACCGGGCCAAAACACTCCTAATAACTGTCCAG; GCAGGACCCACCCCCTGTTTTGTCGAATTCCATTGTTATGGCCCTATTTCCGCCCCTTCC; CTGCCAAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_000351
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000351.3, NP_000342.2
• RefSeq Size: 6366 bp
• RefSeq ORF: 1752 bp
• Locus ID: 412
• UniProt ID: P08842
• Cytogenetics: Xp22.31
• Domains: Sulfatase
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Androgen and estrogen metabolism
• Summary: This gene encodes a multi-pass membrane protein that is localized to the endoplasmic reticulum. It belongs to the sulfatase family and hydrolyzes several 3-beta-hydroxysteroid sulfates, which serve as metabolic precursors for estrogens, androgens, and cholesterol. Mutations in this gene are associated with X-linked ichthyosis (XLI). Alternatively spliced transcript variants resulting from the use of different promoters have been described for this gene (PMID:17601726). [provided by RefSeq, Mar 2016]; Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform 1. It is expressed almost exclusively in the placenta. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.