TSPY3 (NM_001077697) Human Tagged ORF Clone

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SKU
RC210427
TSPY3 (Myc-DDK-tagged)-Human testis specific protein, Y-linked 3 (TSPY3)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TSPY3
Synonyms CT78; TSPY; TSPY1; TSPY10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210427 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCCCTGAGGGCTCGCTGACCTACCGGGTGCCAGAGAGGCTGCGGCAGGGTTTCTGTGGCGTGGGTC
GGGCAGCACAGGCCTTGGTGTGTGCGAGTGCCAAGGAGGGCACCGCCTTCAGGATGGAGGCTGTACAGGA
GGGGGCGGCCGGGGTGGAGAGTGAGCAGGCGGCTTTGGGGGAGGAGGCGGTGCTGCTGTTGGATGACATA
ATGGCGGAGGTGGAGGTGGTGGCGGAGGAGGAGGGCCTCGTGGAGCGGCGGGAGGAGGCCCAGCGGGCAC
AGCAGGCTGTGCCTGGCCCTGGGCCCATGACCCCAGAGTCTGCACTGGAGGAGCTGCTGGCCGTTCAGGT
GGAGCTGGAGCCGGTTAATGCCCAAGCCAGGAAGGCCTTTTCTCGGCAGCGGGAAAAGATGGAGCGGAGG
CGCAAGCCCCAGCTAGACCGCAGAGGCGCCGTCATCCAGAGCGTCCCTGGCTTCTGGGCCAATGTTATTG
CAAACCACCCCCAGATGTCAGCCCTGATCACTGACGAAGATGAAGACATGCTGAGCTACATGGTCAGCCT
GGAGGTGGAAGAAGAGAAGCATCCTGTTCATCTCTGCAAGATCATGTTGTTCTTTCGGAGTAACCCCTAC
TTCCAGAATAAAGTGATTACCAAGGAATATCTGGTGAACATCACAGAATACAGGGCTTCTCATTCCACTC
CAATTGAGTGGTATCCGGATTATGAAGTGGAGGCCTATCGCCGCAGACACCACAACAGCAGCCTTAACTT
CTTCAACTGGTTCTCTGACCACAACTTCGCAGGATCTAACAAGATTGCTGAGATCCTATGTAAGGACCTG
TGGCGCAATCCCCTGCAATACTACAAGAGGATGAAGCCACCTGAAGAGGGAACAGAGACGTCAGGGGACT
CCCAGTTGTTGAGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210427 protein sequence
Red=Cloning site Green=Tags(s)

MRPEGSLTYRVPERLRQGFCGVGRAAQALVCASAKEGTAFRMEAVQEGAAGVESEQAALGEEAVLLLDDI
MAEVEVVAEEEGLVERREEAQRAQQAVPGPGPMTPESALEELLAVQVELEPVNAQARKAFSRQREKMERR
RKPQLDRRGAVIQSVPGFWANVIANHPQMSALITDEDEDMLSYMVSLEVEEEKHPVHLCKIMLFFRSNPY
FQNKVITKEYLVNITEYRASHSTPIEWYPDYEVEAYRRRHHNSSLNFFNWFSDHNFAGSNKIAEILCKDL
WRNPLQYYKRMKPPEEGTETSGDSQLLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001077697
ORF Size 924 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001077697.1, NP_001071165.1
RefSeq Size 1161 bp
RefSeq ORF 927 bp
Locus ID 728137
UniProt ID Q01534, P0CV98, P0CW01
Cytogenetics Yp11.2
MW 35.1 kDa
Summary May be involved in sperm differentiation and proliferation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001077697" in other vectors (4)
SKU Description Size Price
RC210427L3 Lenti ORF clone of Human testis specific protein, Y-linked 3 (TSPY3), Myc-DDK-tagged 10 ug
$600.00
RC210427L4 Lenti ORF clone of Human testis specific protein, Y-linked 3 (TSPY3), mGFP tagged 10 ug
$600.00
RG210427 TSPY3 (tGFP-tagged) - Human testis specific protein, Y-linked 3 (TSPY3) 10 ug
$489.00 $500.00
SC315518 TSPY3 (untagged)-Human testis specific protein, Y-linked 3 (TSPY3) 10 ug
$330.00

Plasmid Map for RC210427

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Cloning scheme for RC210427

Circular map for RC210427

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