ASB16 (NM_080863) Human Tagged ORF Clone

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SKU
RC210418
ASB16 (Myc-DDK-tagged)-Human ankyrin repeat and SOCS box containing 16 (ASB16)
  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ASB16
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210418 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAGAGAGACCTTCCCCTTCACCTCCTCCATGCTGCGCTCTCTCCGCCTGCAGCAGGAGTGGCTGG
AATGGGAGGACCGGCGGCGGGCGGCTGCCCAGCAGTGCCGGAGCCGCAGGTGCCCGTCAAGTCCCCGGGC
CCGACTCACTAGGCCTCACCGTTCCTGCCGAGACCCAGCTGTCCACCATGCCCTCTTCTCCGGCAACCTG
CAGCAGGTCCAAGCCCTGTTCCAAGATGAAGAGGCCGCCAACATGATTGTGGAGACTGTGAGCAACCAGC
TGGCCTGGTCGGCTGAACAGGGGTTCTGGGTGCTGACCCCCAAGACCAAGCAGACGGCACCCCTCGCCAT
CGCTACAGCCCGAGGCTACACAGACTGTGCTCGACACCTGATCCGGCAGGGAGCTGAGCTGGATGCCCGT
GTCGGGGGTCGCGCTGCCTTGCATGAGGCCTGTGCCCGAGCCCAGTTTGACTGTGTGCGGCTGCTGCTGA
CCTTCGGAGCCAAGGCTAATGTGCTGACTGAGGAGGGCACGACTCCTTTGCACCTCTGCACGATCCCCGA
GTCCTTGCAGTGCGCCAAGTTGCTGCTGGAAGCAGGAGCGACGGTGAACCTGGCAGCAGGCGAGAGCCAG
GAGACGCCCCTGCACGTGGCGGCGGCGCGCGGCCTGGAGCAACATGTGGCTCTGTACCTGGAGCATGGCG
CCGACGTGGGCCTGCGCACCAGCCAGGGCGAGACTGCGCTGAACGCGGCGTGCGCTGGGGCCGAGGGCCC
AGGTAGCTGCAGGCGACACCAGGCTGCGGCGCGCCGGCTCCTGGAGGCTGGAGCTGATGCCCGGGCGGCC
GGGCGCAAGCGCCACACGCCGCTGCACAACGCTTGTGCCAGCGGCTGCGGGGGCCTGGCCGAGCTGCTGC
TGCGTTACGGGGCCCGCGCTGAGGTCCCCAATGGGGCGGGCCACACGCCCATGGACTGTGCGCTGCAGGC
CGTCCAGGACGCCCCCAACTGGGAGCCTGAAGTCCTTTTCGCCGCACTGCTGGACTACGGGGCGCAGCCA
GTGCGCCCTGAGATGCTGAAACACTGCGCCAACTTCCCTCGGGCCCTGGAAGTCCTGCTTAATGCCTATC
CTTGTGTCCCATCCTGTGAGACCTGGGTGGAGGCGGTGCTCCCAGAGCTGTGGAAGGAGCACGAAGCCTT
CTACAGCTCGGCCCTGTGCATGGTGAACCAGCCAAGGCAGCTGCAGCACCTGGCCCGACTAGCTGTGCGC
GCTCGGTTGGGAAGCCGCTGCCGGCAGGGTGCCACCCGGCTGCCACTGCCCCCGCTCCTCAGGGACTACC
TGCTGCTGCGTGTGGAGGGGTGCATCCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210418 protein sequence
Red=Cloning site Green=Tags(s)

MARETFPFTSSMLRSLRLQQEWLEWEDRRRAAAQQCRSRRCPSSPRARLTRPHRSCRDPAVHHALFSGNL
QQVQALFQDEEAANMIVETVSNQLAWSAEQGFWVLTPKTKQTAPLAIATARGYTDCARHLIRQGAELDAR
VGGRAALHEACARAQFDCVRLLLTFGAKANVLTEEGTTPLHLCTIPESLQCAKLLLEAGATVNLAAGESQ
ETPLHVAAARGLEQHVALYLEHGADVGLRTSQGETALNAACAGAEGPGSCRRHQAAARRLLEAGADARAA
GRKRHTPLHNACASGCGGLAELLLRYGARAEVPNGAGHTPMDCALQAVQDAPNWEPEVLFAALLDYGAQP
VRPEMLKHCANFPRALEVLLNAYPCVPSCETWVEAVLPELWKEHEAFYSSALCMVNQPRQLQHLARLAVR
ARLGSRCRQGATRLPLPPLLRDYLLLRVEGCIQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080863
ORF Size 1359 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080863.3
RefSeq Size 2139 bp
RefSeq ORF 1362 bp
Locus ID 92591
UniProt ID Q96NS5
Cytogenetics 17q21.31
MW 49.6 kDa
Summary The protein encoded by this gene is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. They contain ankyrin repeat sequence and a SOCS box domain. The SOCS box serves to couple suppressor of cytokine signalling (SOCS) proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_080863" in other vectors (4)
SKU Description Size Price
RC210418L3 Lenti ORF clone of Human ankyrin repeat and SOCS box containing 16 (ASB16), Myc-DDK-tagged 10 ug
$757.00
RC210418L4 Lenti ORF clone of Human ankyrin repeat and SOCS box containing 16 (ASB16), mGFP tagged 10 ug
$757.00
RG210418 ASB16 (tGFP-tagged) - Human ankyrin repeat and SOCS box containing 16 (ASB16) 10 ug
$657.00
SC305867 ASB16 (untagged)-Human ankyrin repeat and SOCS box containing 16 (ASB16) 10 ug
$503.00

Plasmid Map for RC210418

Download sequence for

Cloning scheme for RC210418

Circular map for RC210418

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