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COLQ (NM_005677) Human Tagged ORF Clone

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SKU
RC210401
COLQ (Myc-DDK-tagged)-Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "COLQ or NM_005677"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol COLQ
Synonyms CMS5; EAD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210401 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGC
CGACTTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGG
CCACAAAGCATGCTGCCTGCTGACGCCTCCTCCACCACCACTGTTCCCACCACCATTCTTCAGAGGTGGC
CGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCCCGTGCA
TGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGG
ACCAAAGGGAGAAAAGGGGGAGCTTGGCCGACCAGGAAGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCT
GGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAAGGTGACCTGGGTATGA
TGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATC
CAGAGGTGAAAAGGGTGACCTGGGTCCCAAAGGAGAAAAGGGTTTCCCAGGATTTCCTGGAATGTTGGGG
CAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGGACACCGAGGACCCACAGGAAGACCAG
GAAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTC
TGGTCAACCTGGCCGTCCGGGGCCCCCAGGCCCCCCACCTGCAGGACAACTTATAATGGGACCCAAAGGG
GAAAGAGGATTTCCCGGGCCTCCAGGAAGATGTCTTTGTGGACCCACTATGAATGTGAATAACCCTTCCT
ACGGGGAATCTGTGTATGGGCCCAGTTCCCCGCGAGTTCCTGTGATTTTTGTGGTCAACAACCAGGAGGA
GCTTGAGAGGCTGAACACCCAAAACGCCATTGCCTTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGAC
AGCCTTGGCTGGCTCCCCATCCAGCTGACCCCTTTCTACCCTGTGGATTACACTGCAGACCAGCACGGCA
CCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGTGACGACGGTAACAGCGATGTGGGTGACGACTG
CATCCGCTGTCACCGTGCCTACTGTGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCTCT
GACTTTGGCTACCTGACATGCGAGACCTATCTCCCTGGGTCATATGGAGACCTGCAATGCACCCAGTACT
GCTACATCGACTCCACGCCCTGCCGCTACTTCACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210401 protein sequence
Red=Cloning site Green=Tags(s)

MVVLNPMTLGIYLQLFFLSIVSQPTFINSVLPISAALPSLDQKKRGGHKACCLLTPPPPPLFPPPFFRGG
RSPLLSPDMKNLMLELETSQSPCMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGELGRPGRKGRPGPPGVP
GMPGPIGWPGPEGPRGEKGDLGMMGLPGSRGPMGSKGYPGSRGEKGSRGEKGDLGPKGEKGFPGFPGMLG
QKGEMGPKGEPGIAGHRGPTGRPGKRGKQGQKGDSGVMGPPGKPGPSGQPGRPGPPGPPPAGQLIMGPKG
ERGFPGPPGRCLCGPTMNVNNPSYGESVYGPSSPRVPVIFVVNNQEELERLNTQNAIAFRRDQRSLYFKD
SLGWLPIQLTPFYPVDYTADQHGTCGDGLLQPGEECDDGNSDVGDDCIRCHRAYCGDGHRHEGVEDCDGS
DFGYLTCETYLPGSYGDLQCTQYCYIDSTPCRYFT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005677
ORF Size 1365 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005677.4
RefSeq Size 3007 bp
RefSeq ORF 1368 bp
Locus ID 8292
UniProt ID Q9Y215
Cytogenetics 3p25.1
Protein Families Druggable Genome, Transmembrane
MW 47.8 kDa
Summary This gene encodes the subunit of a collagen-like molecule associated with acetylcholinesterase in skeletal muscle. Each molecule is composed of three identical subunits. Each subunit contains a proline-rich attachment domain (PRAD) that binds an acetylcholinesterase tetramer to anchor the catalytic subunit of the enzyme to the basal lamina. Mutations in this gene are associated with endplate acetylcholinesterase deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
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"NM_005677" in other vectors (6)
SKU Description Size Price
RC210401L1 Lenti ORF clone of Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I, Myc-DDK-tagged 10 ug
$757.00
RC210401L2 Lenti ORF clone of Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I, mGFP tagged 10 ug
$757.00
RC210401L3 Lenti ORF clone of Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I, Myc-DDK-tagged 10 ug
$757.00
RC210401L4 Lenti ORF clone of Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I, mGFP tagged 10 ug
$757.00
RG210401 COLQ (tGFP-tagged) - Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I 10 ug
$489.00 $657.00
SC303680 COLQ (untagged)-Human collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase (COLQ), transcript variant I 10 ug
$457.00

Plasmid Map for RC210401

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210401
Circular map for RC210401

file-alt Citations

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