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CDS1 (NM_001263) Human Tagged ORF Clone

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SKU
RC210375
CDS1 (Myc-DDK-tagged)-Human CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 1 (CDS1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CDS1
Synonyms CDS 1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210375 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGGAGCTGAGGCACCGGGGAAGCTGCCCCGGCCCCAGGGAAGCGGTGTCGCCGCCACACCGCGAGG
GAGAGGCGGCCGGCGGCGACCACGAAACCGAGAGCACCAGCGACAAAGAAACAGATATTGATGACAGATA
TGGAGATTTGGATTCCAGAACAGATTCTGATATTCCGGAAATTCCACCATCCTCAGATAGAACCCCTGAG
ATTCTCAAAAAAGCTCTATCTGGTTTATCTTCAAGGTGGAAAAACTGGTGGATACGTGGAATTCTCACTC
TAACTATGATCTCGTTGTTTTTCCTGATCATCTATATGGGATCCTTCATGCTGATGCTTCTTGTTCTGGG
CATCCAAGTGAAATGCTTCCATGAAATTATCACTATAGGTTATAGAGTCTATCATTCTTATGATCTACCA
TGGTTTAGAACACTAAGTTGGTACTTTCTATTGTGTGTAAACTACTTTTTCTATGGAGAGACTGTAGCTG
ATTATTTTGCTACATTTGTTCAAAGAGAAGAACAACTTCAGTTCCTCATTCGCTACCATAGATTTATATC
ATTTGCCCTCTATCTGGCAGGTTTCTGCATGTTTGTACTGAGTTTGGTGAAGAAACATTATCGTCTGCAG
TTTTATATGTTCGCATGGACTCATGTCACTTTACTGATAACTGTCACTCAGTCACACCTTGTCATCCAAA
ATCTGTTTGAAGGCATGATATGGTTCCTTGTTCCAATATCAAGTGTTATCTGCAATGACATAACTGCTTA
CCTTTTTGGATTTTTTTTTGGGAGAACTCCATTAATTAAGTTGTCTCCTAAAAAGACTTGGGAAGGATTC
ATTGGTGGTTTCTTTTCCACAGTTGTGTTTGGATTCATTGCTGCCTATGTGTTATCCAAATACCAGTACT
TTGTCTGCCCAGTGGAATACCGAAGTGATGTAAACTCCTTCGTGACAGAATGTGAGCCCTCAGAACTTTT
CCAGCTTCAGACTTACTCACTTCCACCCTTTCTAAAGGCAGTCTTGAGACAGGAAAGAGTGAGCTTGTAC
CCTTTCCAGATCCACAGCATTGCACTGTCAACCTTTGCATCTTTAATTGGCCCATTTGGAGGCTTCTTTG
CTAGTGGATTCAAAAGAGCCTTCAAAATCAAGGATTTTGCAAATACCATTCCTGGACATGGTGGGATAAT
GGACAGATTTGATTGTCAGTATTTGATGGCAACTTTTGTACATGTGTACATCACAAGTTTTATAAGGGGC
CCAAATCCCAGCAAAGTGCTACAGCAGTTGTTGGTGCTTCAACCTGAACAGCAGTTAAATATATATAAAA
CCCTGAAGACTCATCTCATTGAGAAAGGAATCCTACAACCCACCTTGAAGGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210375 protein sequence
Red=Cloning site Green=Tags(s)

MLELRHRGSCPGPREAVSPPHREGEAAGGDHETESTSDKETDIDDRYGDLDSRTDSDIPEIPPSSDRTPE
ILKKALSGLSSRWKNWWIRGILTLTMISLFFLIIYMGSFMLMLLVLGIQVKCFHEIITIGYRVYHSYDLP
WFRTLSWYFLLCVNYFFYGETVADYFATFVQREEQLQFLIRYHRFISFALYLAGFCMFVLSLVKKHYRLQ
FYMFAWTHVTLLITVTQSHLVIQNLFEGMIWFLVPISSVICNDITAYLFGFFFGRTPLIKLSPKKTWEGF
IGGFFSTVVFGFIAAYVLSKYQYFVCPVEYRSDVNSFVTECEPSELFQLQTYSLPPFLKAVLRQERVSLY
PFQIHSIALSTFASLIGPFGGFFASGFKRAFKIKDFANTIPGHGGIMDRFDCQYLMATFVHVYITSFIRG
PNPSKVLQQLLVLQPEQQLNIYKTLKTHLIEKGILQPTLKV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001263
ORF Size 1383 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001263.4
RefSeq Size 4546 bp
RefSeq ORF 1386 bp
Locus ID 1040
UniProt ID Q92903, A0A024RDG8
Cytogenetics 4q21.23
Domains CTP_transf_1
Protein Families Transmembrane
Protein Pathways Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
MW 53.3 kDa
Summary Breakdown products of phosphoinositides are ubiquitous second messengers that function downstream of many G protein-coupled receptors and tyrosine kinases regulating cell growth, calcium metabolism, and protein kinase C activity. This gene encodes an enzyme which regulates the amount of phosphatidylinositol available for signaling by catalyzing the conversion of phosphatidic acid to CDP-diacylglycerol. This enzyme is an integral membrane protein localized to two subcellular domains, the matrix side of the inner mitochondrial membrane where it is thought to be involved in the synthesis of phosphatidylglycerol and cardiolipin and the cytoplasmic side of the endoplasmic reticulum where it functions in phosphatidylinositol biosynthesis. Two genes encoding this enzyme have been identified in humans, one mapping to human chromosome 4q21 and a second to 20p13. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001263" in other vectors (4)
SKU Description Size Price
RC210375L3 Lenti ORF clone of Human CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 1 (CDS1), Myc-DDK-tagged 10 ug
$757.00
RC210375L4 Lenti ORF clone of Human CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 1 (CDS1), mGFP tagged 10 ug
$757.00
RG210375 CDS1 (tGFP-tagged) - Human CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 1 (CDS1) 10 ug
$489.00 $657.00
SC119345 CDS1 (untagged)-Human CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 1 (CDS1) 10 ug
$457.00

Plasmid Map for RC210375

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210375
Circular map for RC210375

file-alt Citations

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