KCNE3 (NM_005472) Human Tagged ORF Clone

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SKU
RC210303
KCNE3 (Myc-DDK-tagged)-Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KCNE3
Synonyms BRGDA6; HOKPP; HYPP; MiRP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210303 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACTACCAATGGAACGGAGACCTGGTATGAGAGCCTGCATGCCGTGCTGAAGGCTCTAAATGCCA
CTCTTCACAGCAATTTGCTCTGCCGGCCAGGGCCAGGGCTGGGGCCAGACAACCAGACTGAAGAGAGGCG
GGCCAGCCTACCTGGCCGTGATGACAACTCCTACATGTACATTCTCTTTGTCATGTTTCTATTTGCTGTA
ACTGTGGGCAGCCTCATCCTGGGATACACCCGCTCCCGCAAAGTGGACAAGCGTAGTGACCCCTATCATG
TGTATATCAAGAACCGTGTGTCTATGATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210303 protein sequence
Red=Cloning site Green=Tags(s)

METTNGTETWYESLHAVLKALNATLHSNLLCRPGPGLGPDNQTEERRASLPGRDDNSYMYILFVMFLFAV
TVGSLILGYTRSRKVDKRSDPYHVYIKNRVSMI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005472
ORF Size 309 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005472.5
RefSeq Size 3070 bp
RefSeq ORF 312 bp
Locus ID 10008
UniProt ID Q9Y6H6, Q6IAE6
Cytogenetics 11q13.4
Protein Families Druggable Genome, Ion Channels: Other, Transmembrane
MW 11.7 kDa
Summary Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, isk-related subfamily. This member is a type I membrane protein, and a beta subunit that assembles with a potassium channel alpha-subunit to modulate the gating kinetics and enhance stability of the multimeric complex. This gene is prominently expressed in the kidney. A missense mutation in this gene is associated with hypokalemic periodic paralysis. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_005472" in other vectors (6)
SKU Description Size Price
RC210303L1 Lenti ORF clone of Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3), Myc-DDK-tagged 10 ug
$450.00
RC210303L2 Lenti ORF clone of Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3), mGFP tagged 10 ug
$450.00
RC210303L3 Lenti ORF clone of Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3), Myc-DDK-tagged 10 ug
$450.00
RC210303L4 Lenti ORF clone of Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3), mGFP tagged 10 ug
$450.00
RG210303 KCNE3 (tGFP-tagged) - Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3) 10 ug
$489.00
SC111131 KCNE3 (untagged)-Human potassium voltage-gated channel, Isk-related family, member 3 (KCNE3) 10 ug
$150.00

Plasmid Map for RC210303

Download sequence for

Cloning scheme for RC210303

Circular map for RC210303

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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