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CAPS (NM_004058) Human Tagged ORF Clone

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SKU
RC210271
CAPS (Myc-DDK-tagged)-Human calcyphosine (CAPS), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CAPS
Synonyms CAPS1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210271 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGCCGTGGATGCCACCATGGAGAAACTCCGGGCACAGTGCCTGTCCCGCGGGGCCTCGGGCATCC
AGGGCCTGGCCAGGTTTTTCCGCCAACTAGACCGGGACGGGAGCAGATCCCTGGACGCTGATGAGTTCCG
GCAGGGTCTGGCCAAACTCGGGCTGGTGCTGGACCAGGCGGAGGCAGAGGGTGTGTGCAGGAAGTGGGAC
CGCAATGGCAGCGGGACGCTGGATCTGGAGGAGTTCCTTCGGGCGCTGCGGCCCCCCATGTCCCAGGCCC
GGGAGGCTGTCATCGCAGCTGCATTTGCCAAGCTGGACCGCAGCGGGGACGGCGTCGTGACGGTGGACGA
CCTCCGCGGGGTGTACAGTGGCCGTGCCCACCCCAAGGTGCGCAGTGGGGAGTGGACCGAGGACGAGGTG
CTGCGCCGCTTCCTGGACAACTTCGACTCCTCTGAGAAGGACGGGCAGGTCACACTGGCGGAATTCCAGG
ACTACTACAGCGGCGTGAGTGCCTCCATGAACACGGATGAGGAGTTCGTGGCCATGATGACCAGTGCCTG
GCAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210271 protein sequence
Red=Cloning site Green=Tags(s)

MDAVDATMEKLRAQCLSRGASGIQGLARFFRQLDRDGSRSLDADEFRQGLAKLGLVLDQAEAEGVCRKWD
RNGSGTLDLEEFLRALRPPMSQAREAVIAAAFAKLDRSGDGVVTVDDLRGVYSGRAHPKVRSGEWTEDEV
LRRFLDNFDSSEKDGQVTLAEFQDYYSGVSASMNTDEEFVAMMTSAWQL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004058
ORF Size 567 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004058.5
RefSeq Size 1613 bp
RefSeq ORF 570 bp
Locus ID 828
UniProt ID Q13938, A0A384NYV7, Q96ET4
Cytogenetics 19p13.3
MW 21 kDa
Summary This gene encodes a calcium-binding protein, which may play a role in the regulation of ion transport. A similar protein was first described as a potentially important regulatory protein in the dog thyroid and was termed as R2D5 antigen in rabbit. Alternative splicing of this gene generates two transcript variants. provided by RefSeq, Jul 2008
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"NM_004058" in other vectors (4)
SKU Description Size Price
RC210271L3 Lenti ORF clone of Human calcyphosine (CAPS), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC210271L4 Lenti ORF clone of Human calcyphosine (CAPS), transcript variant 1, mGFP tagged 10 ug
$600.00
RG210271 CAPS (tGFP-tagged) - Human calcyphosine (CAPS), transcript variant 1 10 ug
$489.00 $500.00
SC303427 CAPS (untagged)-Human calcyphosine (CAPS), transcript variant 1 10 ug
$330.00

Plasmid Map for RC210271

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210271
Circular map for RC210271

file-alt Citations

CAPS1 Negatively Regulates Hepatocellular Carcinoma Development through Alteration of Exocytosis-Associated Tumor Microenvironment
Xue, Tang, Dong et al
Int J Mol Sci (2016) 17 (10) DOI: 10.3390/ijms17101626
AI Summary: OriGene's CAPS1 expression vectors and control vectors were used to overexpress CAPS1 in hepatoma cell lines, enabling the study of CAPS1's tumor-suppressive effects in hepatocellular carcinoma.
Snippet: Expression vectors of CAPS1 or control vectors (Origene Technologies, Rockville, MD, USA) were transfected into Huh7 and HCCLM3 cells using lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA)
FigureFigureFigure
Showing 1-1 of 1 papers.
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