EXOC2 (NM_018303) Human Tagged ORF Clone

SKU RC210263

EXOC2 (Myc-DDK-tagged)-Human exocyst complex component 2 (EXOC2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: EXOC2
• Synonyms: NEDFACH; SEC5; SEC5L1; Sec5p
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210263 representing NM_018303; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCTCGATCACGACAACCCCCCCTTGTGACCGGCATCTCTCCAAATGAAGGGATACCATGGACGAAGG; TCACAATCAGGGGAGAAAATCTGGGGACTGGCCCCACCGACCTCATAGGCTTGACCATTTGTGGACATAA; TTGCCTCCTGACGGCAGAATGGATGTCTGCAAGTAAAATAGTATGTCGAGTGGGACAAGCCAAAAATGAC; AAAGGAGACATTATTGTCACCACTAAGTCAGGTGGCAGAGGAACCTCAACAGTCTCTTTCAAGCTACTCA; AACCTGAGAAAATAGGCATTTTGGATCAGTCTGCTGTGTGGGTTGATGAAATGAATTATTATGATATGCG; CACTGACAGGAACAAAGGAATTCCGCCCTTGTCCTTACGTCCTGCTAACCCGCTTGGCATTGAGATTGAA; AAAAGTAAATTTTCGCAGAAGGACTTAGAAATGCTATTCCATGGAATGAGTGCTGATTTTACAAGTGAGA; ATTTCTCAGCAGCCTGGTATCTTATAGAGAATCACTCAAACACCAGTTTTGAGCAGCTCAAAATGGCAGT; CACCAACCTAAAGAGACAGGCTAACAAGAAGAGTGAGGGCAGCCTGGCCTATGTGAAAGGCGGTCTCAGT; ACATTCTTCGAAGCACAGGATGCCCTCTCAGCCATCCATCAAAAACTAGAAGCAGATGGAACGGAAAAAG; TAGAAGGATCCATGACGCAGAAACTGGAGAATGTTCTGAACAGAGCAAGTAATACTGCAGACACATTGTT; TCAAGAAGTATTAGGTCGGAAAGACAAGGCAGATTCCACTAGAAATGCACTCAATGTGCTTCAGCGATTT; AAGTTTCTTTTCAACCTTCCTCTAAATATTGAAAGGAATATTCAAAAGGGTGATTATGATGTGGTTATTA; ATGATTATGAAAAGGCCAAGTCACTTTTTGGGAAAACGGAGGTGCAAGTTTTCAAGAAATATTATGCTGA; AGTAGAAACAAGGATTGAAGCTTTAAGAGAATTACTTCTGGATAAATTGCTTGAGACACCATCAACTTTA; CATGACCAAAAACGTTACATAAGGTACCTGTCTGACCTTCATGCGTCTGGTGACCCTGCTTGGCAATGCA; TTGGAGCCCAACACAAGTGGATCCTTCAGCTCATGCACAGTTGCAAAGAGGGCTACGTGAAAGATCTGAA; AGGTAACCCAGGCCTGCACAGTCCCATGTTGGATCTTGATAATGATACACGTCCCTCAGTGTTGGGCCAT; CTCAGTCAGACAGCGTCCCTGAAGAGGGGCAGCAGCTTTCAGTCTGGTCGAGACGACACGTGGAGATACA; AAACTCCCCACAGGGTGGCCTTTGTTGAAAAATTGACAAAACTCGTCTTGAGCCAGCTGCCTAACTTCTG; GAAACTCTGGATCTCCTACGTTAATGGAAGCCTCTTCAGTGAGACTGCTGAGAAGTCAGGCCAGATTGAA; AGATCAAAGAATGTAAGGCAAAGACAAAATGATTTTAAGAAAATGATTCAGGAAGTAATGCACTCCCTGG; TGAAGCTTACCCGCGGAGCCCTGCTTCCCCTCAGCATCCGGGATGGGGAAGCCAAGCAGTACGGAGGCTG; GGAGGTGAAGTGCGAGCTCTCCGGACAGTGGCTCGCTCACGCCATCCAGACTGTAAGACTTACTCATGAA; TCGTTGACTGCCCTTGAAATTCCTAATGACCTGTTACAGACTATCCAGGATCTCATCTTGGATCTCCGAG; TACGTTGCGTAATGGCCACGTTGCAGCACACGGCGGAAGAAATAAAGAGATTAGCTGAAAAAGAAGACTG; GATTGTTGACAATGAAGGACTGACTTCTCTACCATGTCAGTTTGAACAGTGCATCGTGTGTTCTCTGCAG; TCACTGAAGGGGGTTCTGGAGTGCAAGCCGGGAGAGGCCAGTGTCTTCCAACAACCTAAAACACAGGAGG; AGGTTTGCCAGCTAAGCATCAATATAATGCAGGTTTTTATATACTGTCTGGAACAGTTGAGCACCAAGCC; TGATGCAGATATAGATACTACACATCTCTCTGTTGATGTTTCTTCCCCTGACTTGTTTGGAAGTATCCAT; GAAGACTTCAGCTTGACCTCAGAACAGCGCCTTTTGATAGTCCTAAGTAATTGCTGCTATCTAGAACGTC; ACACCTTCCTAAATATCGCAGAACATTTTGAAAAGCACAACTTCCAGGGAATAGAAAAAATCACACAGGT; TAGCATGGCCTCATTGAAAGAACTAGATCAAAGACTCTTTGAAAATTACATCGAGTTGAAAGCAGATCCC; ATCGTTGGCTCCTTAGAACCTGGAATTTATGCAGGATATTTTGATTGGAAGGACTGCCTGCCTCCAACAG; GTGTCAGAAACTATTTAAAAGAAGCACTGGTGAATATAATTGCCGTGCATGCAGAGGTGTTCACCATTTC; CAAAGAACTGGTCCCTCGGGTACTATCCAAGGTGATAGAAGCAGTTTCTGAAGAGCTCAGTCGACTGATG; CAGTGTGTTTCATCCTTCAGCAAAAATGGAGCTTTACAGGCGAGACTTGAAATCTGTGCTTTGAGGGACA; CTGTGGCTGTTTACCTGACACCCGAAAGCAAGTCAAGTTTTAAGCAGGCTTTGGAAGCCCTGCCCCAGCT; TTCCAGTGGAGCAGATAAAAAGTTACTGGAAGAGCTCCTGAACAAGTTCAAGAGTAGCATGCACTTGCAG; CTCACCTGTTTCCAAGCAGCTTCTTCAACCATGATGAAAACA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210263 representing NM_018303; Red=Cloning site Green=Tags(s); ; MSRSRQPPLVTGISPNEGIPWTKVTIRGENLGTGPTDLIGLTICGHNCLLTAEWMSASKIVCRVGQAKND; KGDIIVTTKSGGRGTSTVSFKLLKPEKIGILDQSAVWVDEMNYYDMRTDRNKGIPPLSLRPANPLGIEIE; KSKFSQKDLEMLFHGMSADFTSENFSAAWYLIENHSNTSFEQLKMAVTNLKRQANKKSEGSLAYVKGGLS; TFFEAQDALSAIHQKLEADGTEKVEGSMTQKLENVLNRASNTADTLFQEVLGRKDKADSTRNALNVLQRF; KFLFNLPLNIERNIQKGDYDVVINDYEKAKSLFGKTEVQVFKKYYAEVETRIEALRELLLDKLLETPSTL; HDQKRYIRYLSDLHASGDPAWQCIGAQHKWILQLMHSCKEGYVKDLKGNPGLHSPMLDLDNDTRPSVLGH; LSQTASLKRGSSFQSGRDDTWRYKTPHRVAFVEKLTKLVLSQLPNFWKLWISYVNGSLFSETAEKSGQIE; RSKNVRQRQNDFKKMIQEVMHSLVKLTRGALLPLSIRDGEAKQYGGWEVKCELSGQWLAHAIQTVRLTHE; SLTALEIPNDLLQTIQDLILDLRVRCVMATLQHTAEEIKRLAEKEDWIVDNEGLTSLPCQFEQCIVCSLQ; SLKGVLECKPGEASVFQQPKTQEEVCQLSINIMQVFIYCLEQLSTKPDADIDTTHLSVDVSSPDLFGSIH; EDFSLTSEQRLLIVLSNCCYLERHTFLNIAEHFEKHNFQGIEKITQVSMASLKELDQRLFENYIELKADP; IVGSLEPGIYAGYFDWKDCLPPTGVRNYLKEALVNIIAVHAEVFTISKELVPRVLSKVIEAVSEELSRLM; QCVSSFSKNGALQARLEICALRDTVAVYLTPESKSSFKQALEALPQLSSGADKKLLEELLNKFKSSMHLQ; LTCFQAASSTMMKT; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018303
• ORF Size: 2772 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018303.6
• RefSeq Size: 4458 bp
• RefSeq ORF: 2775 bp
• Locus ID: 55770
• UniProt ID: Q96KP1
• Cytogenetics: 6p25.3
• Domains: IPT
• MW: 103.9 kDa
• Summary: The protein encoded by this gene is a component of the exocyst complex, a multi-protein complex essential for the polarized targeting of exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and the functions of the exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. This interaction has been shown to mediate filopodia formation in fibroblasts. This protein has been shown to interact with the Ral subfamily of GTPases and thereby mediate exocytosis by tethering vesicles to the plasma membrane. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]