NSUN5C (NM_032158) Human Tagged ORF Clone

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SKU
RC210254
NSUN5C (Myc-DDK-tagged)-Human NOL1/NOP2/Sun domain family, member 5C (NSUN5C), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NSUN5C
Synonyms DKFZp434K058; DKFZp666P104; FLJ11626; MGC15057; MGC129801; NOL1/NOP2/Sun domain family, member 5C; NOL1R2; WBSCR20B; WBSCR20C; Williams Beuren syndrome chromosome region 20C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210254 representing NM_032158
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGAGCTGCTGGTGTTTCCCGCCCAGACAGATCTGCATGAACACCCACTGTACCGGGCCGGACACC
TCATTCTGCAGGACAGGGCCAGCTGTCTCCCAGCCATGCTGCTGGACCCCCGCCAGGCTCCCATGTCATG
GATGCCTGTGCCACCCCAGGCAATAAAGACCAGTCACTTGGCTGCTCTTCTGAAGAACCAAGGGAAGATC
TTTGCCTTTGACCTGGATGCCAGGCGGCTGGCATCCATGGCCACGCTGCTGGCCTGGGCTGGCGTCTCCT
GCTGTGAGCTGGCTGAGGAGGACTTCCTGGCGGTCTCCCCCTTAGATCCGCGCTATCGTGAGGTCCACTA
TGTCCTGCTGGATCCTTCCTGCAGTGGCTCGGGTATGCCGAGCAGACAGCTGGAGGAGCCCGGGGCAGGG
ACACCTAGCCCGGTGCGTCTGCATGCCCTGGCAGGGTTCCAGCAGCGAGCCCTGTGCCACGCGCTCACTT
TCCCTTCCCTGCAGCGGCTCGTCTACTCCATGTGCTCCCTCTGCCAGGAGGAGAATGAAGACATGGTACA
AGATGCGCTGCAGCAGAACCCGGGCGCCTTCAGGCTAGCTCCCGCCCTGCCTGCCCGGCCCCACCGAGGC
CTGAGCACGTTCCCGGGTGCCGAGCACTGCCTCCGGGCTTCCCCCAAGACCACGCTTAGCGGTGGCTTCT
TCGTTGCTGTAATTGAACGGGTCGAGATGCCGACCTCAGCCTCACAGGCCAAAGCATCAGCACCAGAACG
CACACCCAGCCCAGCCCCAAAGAGAAAGAAGAGAGCAAAAAGCTGCAGCCGGTGCTTGCACACCGCCTTG
CACATAGCAGAGGCTCCGGGCTCACTCCTTCCTGGTGGGAAAGGAAGATGCCTGTCCTCTCCGTGGAAGA
CCCTGGGCCCTCACCGCAGGCAGCAGTTTGCGTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210254 representing NM_032158
Red=Cloning site Green=Tags(s)

MPELLVFPAQTDLHEHPLYRAGHLILQDRASCLPAMLLDPRQAPMSWMPVPPQAIKTSHLAALLKNQGKI
FAFDLDARRLASMATLLAWAGVSCCELAEEDFLAVSPLDPRYREVHYVLLDPSCSGSGMPSRQLEEPGAG
TPSPVRLHALAGFQQRALCHALTFPSLQRLVYSMCSLCQEENEDMVQDALQQNPGAFRLAPALPARPHRG
LSTFPGAEHCLRASPKTTLSGGFFVAVIERVEMPTSASQAKASAPERTPSPAPKRKKRAKSCSRCLHTAL
HIAEAPGSLLPGGKGRCLSSPWKTLGPHRRQQFAF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032158
ORF Size 945 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032158.3, NP_115534.2
RefSeq Size 1760 bp
RefSeq ORF 947 bp
Locus ID 260294
Cytogenetics 7q11.23
MW 34.2 kDa
Summary This locus represents a transcribed pseudogene of a nearby locus on chromosome 7, which encodes a putative methyltransferase. There is also a third closely related pseudogene locus in this region. There is extensive alternative splicing at this locus. provided by RefSeq, Jul 2013
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Documents
Resources
"NM_032158" in other vectors (4)
SKU Description Size Price
RC210254L3 Lenti ORF clone of Human NOL1/NOP2/Sun domain family, member 5C (NSUN5C), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC210254L4 Lenti ORF clone of Human NOL1/NOP2/Sun domain family, member 5C (NSUN5C), transcript variant 1, mGFP tagged 10 ug
$600.00
RG210254 NSUN5C (tGFP-tagged) - Human NOL1/NOP2/Sun domain family, member 5C (NSUN5C), transcript variant 1 10 ug
$500.00
SC312926 NSUN5C (untagged)-Human NOL1/NOP2/Sun domain family, member 5C (NSUN5C), transcript variant 1 10 ug
$330.00

Plasmid Map for RC210254

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Cloning scheme for RC210254

Circular map for RC210254

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