MAGEB3 (NM_002365) Human Tagged ORF Clone

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SKU
RC210237
MAGEB3 (Myc-DDK-tagged)-Human melanoma antigen family B, 3 (MAGEB3)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAGEB3
Synonyms CT3.5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210237 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCGGGGTCAGAAGAGTACGCTCCATGCACGTGAGAAACGCCAGCAGACCCGGGGTCAGACCCAGG
ATCACCAGGGTGCTCAGATCACTGCAACTAACAAGAAAAAAGTATCCTTTTCATCCCCTCTTATTTTGGG
GGCTACTATCCAGAAAAAGTCTGCTGGTAGGTCACGTAGTGCTCTCAAGAAGCCTCAGAGAGCACTATCC
ACCACTACATCTGTAGATGTTTCTTACAAAAAGTCATACAAGGGAGCCAACAGCAAAATTGAGAAAAAGC
AAAGCTTCTCTCAGGGTCTATCCTCCACTGTGCAGTCTCGCACAGACCCTCTAATCATGAAGACAAATAT
GTTGGTGCAGTTCCTGATGGAAATGTACAAGATGAAAAAGCCCATTATGAAAGCAGATATGCTAAAAATT
GTCCAAAAAAGCCATAAGAATTGCTTCCCTGAGATCCTTAAAAAAGCTTCTTTCAACATGGAGGTGGTAT
TTGGTGTTGATTTAAAGAAAGTTGATTCTACCAAGGACTCCTATGTCCTTGTCAGCAAAATGGATCTCCC
CAACAATGGGACAGTGACTCGTGGGAGGGGATTTCCCAAGACAGGTCTCCTGCTGAATCTCCTGGGCGTG
ATCTTCATGAAGGGCAACTGTGCCACTGAGGAGAAGATCTGGGAATTCCTGAATAAGATGAGAATATATG
ATGGGAAGAAACACTTCATATTTGGGGAGCCCAGAAAGCTCATCACCCAAGATTTGGTGAAGCTTAAATA
CCTGGAGTACCGACAAGTGCCCAACAGTAATCCTGCACGCTATGAATTCCTGTGGGGTCCAAGAGCCCAT
GCTGAAACCAGCAAGATGAAGGTCCTGGAGTTTTGGGCCAAGGTCAATAAAACTGTCCCCAGTGCGTTCC
AGTTCTGGTATGAAGAGGCTTTGAGAGATGAGGAAGAAAGAGTCCAAGCTGCAGCTATGCTCAATGATGG
CAGTAGTGCCATGGGCAGAAAGTGTTCCAAGGCCAAGGCTAGCAGCTCTTCCCACGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210237 protein sequence
Red=Cloning site Green=Tags(s)

MPRGQKSTLHAREKRQQTRGQTQDHQGAQITATNKKKVSFSSPLILGATIQKKSAGRSRSALKKPQRALS
TTTSVDVSYKKSYKGANSKIEKKQSFSQGLSSTVQSRTDPLIMKTNMLVQFLMEMYKMKKPIMKADMLKI
VQKSHKNCFPEILKKASFNMEVVFGVDLKKVDSTKDSYVLVSKMDLPNNGTVTRGRGFPKTGLLLNLLGV
IFMKGNCATEEKIWEFLNKMRIYDGKKHFIFGEPRKLITQDLVKLKYLEYRQVPNSNPARYEFLWGPRAH
AETSKMKVLEFWAKVNKTVPSAFQFWYEEALRDEEERVQAAAMLNDGSSAMGRKCSKAKASSSSHA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002365
ORF Size 1038 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002365.4
RefSeq Size 2310 bp
RefSeq ORF 1041 bp
Locus ID 4114
UniProt ID O15480
Cytogenetics Xp21.2
MW 39.2 kDa
Summary This gene is a MAGE-B subfamily member of the MAGE gene family. MAGE family member proteins direct the expression of tumor antigens recognized on a human melanoma by autologous cytolytic T lymphocytes. There are two known clusters of MAGE genes on chromosome X. The members of the MAGE-A subfamily are located in the Xq28 region, while the members of the MAGE-B subfamily are clustered in the Xp21 region. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_002365" in other vectors (4)
SKU Description Size Price
RC210237L3 Lenti ORF clone of Human melanoma antigen family B, 3 (MAGEB3), Myc-DDK-tagged 10 ug
$757.00
RC210237L4 Lenti ORF clone of Human melanoma antigen family B, 3 (MAGEB3), mGFP tagged 10 ug
$757.00
RG210237 MAGEB3 (tGFP-tagged) - Human melanoma antigen family B, 3 (MAGEB3) 10 ug
$489.00 $657.00
SC303187 MAGEB3 (untagged)-Human melanoma antigen family B, 3 (MAGEB3) 10 ug
$457.00

Plasmid Map for RC210237

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Cloning scheme for RC210237

Circular map for RC210237

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