GPRC5D (NM_018654) Human Tagged ORF Clone

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SKU
RC210138
GPRC5D (Myc-DDK-tagged)-Human G protein-coupled receptor, family C, group 5, member D (GPRC5D)
  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GPRC5D
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210138 representing NM_018654
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACAAGGACTGCATCGAGTCCACTGGAGACTATTTTCTTCTCTGTGACGCCGAGGGGCCATGGGGCA
TCATTCTGGAGTCCCTGGCCATACTTGGCATCGTGGTCACAATTCTGCTACTCTTAGCATTTCTCTTCCT
CATGCGAAAGATCCAAGACTGCAGCCAGTGGAATGTCCTCCCCACCCAGCTCCTCTTCCTCCTGAGTGTC
CTGGGGCTCTTCGGACTCGCTTTTGCCTTCATCATCGAGCTCAATCAACAAACTGCCCCCGTACGCTACT
TTCTCTTTGGGGTTCTCTTTGCTCTCTGTTTCTCATGCCTCTTAGCTCATGCCTCCAATCTAGTGAAGCT
GGTTCGGGGTTGTGTCTCCTTCTCCTGGACGACAATTCTGTGCATTGCTATTGGTTGCAGTCTGTTGCAA
ATCATTATTGCCACTGAGTATGTGACTCTCATCATGACCAGAGGTATGATGTTTGTGAATATGACACCCT
GCCAGCTCAATGTGGACTTTGTTGTACTCCTGGTCTATGTCCTCTTCCTGATGGCCCTCACATTCTTCGT
CTCCAAAGCCACCTTCTGTGGCCCGTGTGAGAACTGGAAGCAGCATGGAAGGCTCATCTTTATCACTGTG
CTCTTCTCCATCATCATCTGGGTGGTGTGGATCTCCATGCTCCTGAGAGGCAACCCGCAGTTCCAGCGAC
AGCCCCAGTGGGATGACCCGGTCGTCTGCATTGCTCTGGTCACCAACGCATGGGTTTTCCTGCTGCTGTA
CATCGTCCCTGAGCTCTGCATTCTCTACAGATCGTGTAGACAGGAGTGCCCTTTACAAGGCAATGCCTGC
CCCGTCACAGCCTACCAACACAGCTTCCAAGTGGAGAACCAGGAGCTCTCCAGAGCCCGAGACAGTGATG
GAGCTGAGGAGGATGTAGCATTAACTTCATATGGTACTCCCATTCAGCCGCAGACTGTTGATCCCACACA
AGAGTGTTTCATCCCACAGGCTAAACTAAGCCCCCAGCAAGATGCAGGAGGAGTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210138 representing NM_018654
Red=Cloning site Green=Tags(s)

MYKDCIESTGDYFLLCDAEGPWGIILESLAILGIVVTILLLLAFLFLMRKIQDCSQWNVLPTQLLFLLSV
LGLFGLAFAFIIELNQQTAPVRYFLFGVLFALCFSCLLAHASNLVKLVRGCVSFSWTTILCIAIGCSLLQ
IIIATEYVTLIMTRGMMFVNMTPCQLNVDFVVLLVYVLFLMALTFFVSKATFCGPCENWKQHGRLIFITV
LFSIIIWVVWISMLLRGNPQFQRQPQWDDPVVCIALVTNAWVFLLLYIVPELCILYRSCRQECPLQGNAC
PVTAYQHSFQVENQELSRARDSDGAEEDVALTSYGTPIQPQTVDPTQECFIPQAKLSPQQDAGGV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_018654
ORF Size 1035 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018654.1, NP_061124.1
RefSeq Size 1038 bp
RefSeq ORF 1038 bp
Locus ID 55507
UniProt ID Q9NZD1
Cytogenetics 12p13.1
Protein Families Druggable Genome, GPCR, Transmembrane
MW 38.6 kDa
Summary The protein encoded by this gene is a member of the G protein-coupled receptor family; however, the specific function of this gene has not yet been determined. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_018654" in other vectors (4)
SKU Description Size Price
RC210138L3 Lenti ORF clone of Human G protein-coupled receptor, family C, group 5, member D (GPRC5D), Myc-DDK-tagged 10 ug
$757.00
RC210138L4 Lenti ORF clone of Human G protein-coupled receptor, family C, group 5, member D (GPRC5D), mGFP tagged 10 ug
$757.00
RG210138 GPRC5D (tGFP-tagged) - Human G protein-coupled receptor, family C, group 5, member D (GPRC5D) 10 ug
$489.00 $657.00
SC310520 GPRC5D (untagged)-Human G protein-coupled receptor, family C, group 5, member D (GPRC5D) 10 ug
$457.00

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