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EIF4EBP3 (NM_003732) Human Tagged ORF Clone

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SKU
RC210053
EIF4EBP3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4E binding protein 3 (EIF4EBP3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "EIF4EBP3 or NM_003732"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EIF4EBP3
Synonyms 4E-BP3; 4EBP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210053 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAACGTCCACTAGCTGCCCGATTCCCGGGGGCCGGGACCAGCTGCCCGACTGCTACAGCACCACGC
CGGGGGGCACGCTATACGCCACTACCCCCGGAGGCACCAGGATCATCTACGACCGAAAGTTCCTGCTGGA
GTGCAAGAACTCACCCATTGCCCGGACACCCCCCTGCTGCCTCCCTCAGATTCCCGGGGTCACAACTCCT
CCAACAGCCCCTCTCTCCAAGCTGGAGGAGCTGAAGGAGCAGGAGACAGAGGAAGAGATACCCGATGACG
CACAATTTGAAATGGACATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210053 protein sequence
Red=Cloning site Green=Tags(s)

MSTSTSCPIPGGRDQLPDCYSTTPGGTLYATTPGGTRIIYDRKFLLECKNSPIARTPPCCLPQIPGVTTP
PTAPLSKLEELKEQETEEEIPDDAQFEMDI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003732
ORF Size 300 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003732.3
RefSeq Size 728 bp
RefSeq ORF 303 bp
Locus ID 8637
UniProt ID O60516
Cytogenetics 5q31.3
MW 10.9 kDa
Summary This gene encodes a member of the EIF4EBP family, which consists of proteins that bind to eukaryotic translation initiation factor 4E and regulate its assembly into EIF4F, the multi-subunit translation initiation factor that recognizes the mRNA cap structure. Read-through transcription from the neighboring upstream gene (MASK or ANKHD1) generates a transcript (MASK-BP3) that encodes a protein comprised of the MASK protein sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. provided by RefSeq, Oct 2010
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"NM_003732" in other vectors (4)
SKU Description Size Price
RC210053L3 Lenti ORF clone of Human eukaryotic translation initiation factor 4E binding protein 3 (EIF4EBP3), Myc-DDK-tagged 10 ug
$450.00
RC210053L4 Lenti ORF clone of Human eukaryotic translation initiation factor 4E binding protein 3 (EIF4EBP3), mGFP tagged 10 ug
$450.00
RG210053 EIF4EBP3 (tGFP-tagged) - Human eukaryotic translation initiation factor 4E binding protein 3 (EIF4EBP3) 10 ug
$489.00
SC117797 EIF4EBP3 (untagged)-Human eukaryotic translation initiation factor 4E binding protein 3 (EIF4EBP3) 10 ug
$150.00

Plasmid Map for RC210053

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC210053
Circular map for RC210053

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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