CAAP1 (NM_024828) Human Tagged ORF Clone

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SKU
RC210037
CAAP1 (Myc-DDK-tagged)-Human chromosome 9 open reading frame 82 (C9orf82), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CAAP1
Synonyms C9orf82; CAAP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210037 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGGGAAAAAGTCCTCCCGGGAGAAACGGCGCAAACGTAGCAGTCAGGAGGCGGCCGCAGCGCTCG
CGGCCCCGGACATCGTACCCGCGTTGGCCAGCGGCAGCAGTGGAAGCACTAGCGGCTGCGGGAGCGCCGG
GGGCTGCGGGAGCGTCAGCTGCTGTGGGAACGCCAATTTTAGTGGAAGTGTCACCGGCGGTGGGAGCGGC
GGCAGCTGTTGGGGCGGGAGCAGCGTGGAGCGCAGCGAGCGCCGGAAGCGGAGGAGTACCGACTCTTCCA
GCGTCTCGGGCTCCTTGCAGCAGGAAACTAAATATATTTTGCCAACTTTGGAAAAAGAATTATTCTTGGC
AGAGCACAGTGACCTTGAAGAAGGTGGACTGGACCTGACTGTGTCATTGAAACCAGTTAGTTTCTATATA
TCAGACAAAAAAGAAATGCTTCAGCAGTGCTTCTGTATTATAGGAGAGAAAAAGTTACAGAAGATGCTTC
CTGATGTGTTAAAGAACTGTTCAATAGAAGAAATTAAAAAACTATGCCAGGAACAGTTAGAGCTCCTGTC
TGAAAAAAAAATTTTGAAGATTCTTGAGGGTGACAATGGAATGGACTCTGATATGGAAGAGGAAGCAGAT
GATGGTTCTAAGATGGGATCTGATTTAGTCAGTCAGCAAGACATCTGTATAGATTCTGCTTCATCCGTGA
GAGAGAATAAGCAACCTGAAGGTTTGGAATTAAAACAAGGAAAAGGGGAAGATAGTGATGTACTCAGTAT
AAATGCAGATGCTTATGACAGCGACATAGAAGGCCCATGCAACGAAGAAGCAGCTGCTCCCGAGGCACCA
GAAAATACAGTCCAAAGTGAAGCTGGTCAGATAAATGACCTGGAGAAAGACATTGAGAAAAGTGTGAATG
AGATTCTAGGACTGGCAGAGTCTAGCCCAAACGAACCCAAAGCAGCCACCCTGGCTGTTCCTCCACCAGA
AGATGTTCAACCTTCTGCACAGCAACTGGAGCTGCTAGAACTTGAGATGAGGGCAAGAGCGATTAAAGCC
CTAATGAAAGCTGGTGATATAAAAAAGCCAGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210037 protein sequence
Red=Cloning site Green=Tags(s)

MTGKKSSREKRRKRSSQEAAAALAAPDIVPALASGSSGSTSGCGSAGGCGSVSCCGNANFSGSVTGGGSG
GSCWGGSSVERSERRKRRSTDSSSVSGSLQQETKYILPTLEKELFLAEHSDLEEGGLDLTVSLKPVSFYI
SDKKEMLQQCFCIIGEKKLQKMLPDVLKNCSIEEIKKLCQEQLELLSEKKILKILEGDNGMDSDMEEEAD
DGSKMGSDLVSQQDICIDSASSVRENKQPEGLELKQGKGEDSDVLSINADAYDSDIEGPCNEEAAAPEAP
ENTVQSEAGQINDLEKDIEKSVNEILGLAESSPNEPKAATLAVPPPEDVQPSAQQLELLELEMRARAIKA
LMKAGDIKKPA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024828
ORF Size 1083 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024828.2, NP_079104.2
RefSeq Size 2833 bp
RefSeq ORF 1086 bp
Locus ID 79886
UniProt ID Q9H8G2
Cytogenetics 9p21.2
MW 38.4 kDa
Summary Anti-apoptotic protein that modulates a caspase-10 dependent mitochondrial caspase-3/9 feedback amplification loop.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_024828" in other vectors (4)
SKU Description Size Price
RC210037L3 Lenti ORF clone of Human chromosome 9 open reading frame 82 (C9orf82), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC210037L4 Lenti ORF clone of Human chromosome 9 open reading frame 82 (C9orf82), transcript variant 1, mGFP tagged 10 ug
$757.00
RG210037 CAAP1 (tGFP-tagged) - Human chromosome 9 open reading frame 82 (C9orf82), transcript variant 1 10 ug
$489.00 $657.00
SC321498 CAAP1 (untagged)-Human chromosome 9 open reading frame 82 (C9orf82), transcript variant 1 10 ug
$457.00

Plasmid Map for RC210037

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Cloning scheme for RC210037

Circular map for RC210037

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