AP1S2 (NM_003916) Human Tagged ORF Clone

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SKU
RC210030
AP1S2 (Myc-DDK-tagged)-Human adaptor-related protein complex 1, sigma 2 subunit (AP1S2)
  $225.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AP1S2
Synonyms DC22; MRX59; MRXS5; MRXS21; MRXSF; PGS; SIGMA1B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC210030 representing NM_003916
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGTTTATGTTGCTTTTTAGTCGTCAGGGAAAGCTTCGACTGCAAAAATGGTATGTCCCACTATCAG
ACAAAGAGAAGAAAAAGATCACAAGAGAACTTGTTCAGACCGTTTTAGCACGGAAACCTAAAATGTGCAG
CTTCCTTGAGTGGCGAGATCTGAAGATTGTTTACAAAAGATATGCTAGTCTGTATTTTTGCTGTGCTATT
GAGGATCAGGACAATGAACTAATTACCCTGGAAATAATTCATCGTTATGTGGAATTACTTGACAAGTATT
TCGGCAGTGTCTGTGAACTAGATATCATCTTTAATTTTGAGAAGGCTTATTTTATTTTGGATGAGTTTCT
TTTGGGAGGGGAAGTTCAGGAAACATCCAAGAAAAATGTCCTTAAAGCAATTGAGCAGGCTGATCTACTG
CAGGAGGAAGCTGAAACCCCACGTAGTGTTCTTGAAGAAATTGGACTGACA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC210030 representing NM_003916
Red=Cloning site Green=Tags(s)

MQFMLLFSRQGKLRLQKWYVPLSDKEKKKITRELVQTVLARKPKMCSFLEWRDLKIVYKRYASLYFCCAI
EDQDNELITLEIIHRYVELLDKYFGSVCELDIIFNFEKAYFILDEFLLGGEVQETSKKNVLKAIEQADLL
QEEAETPRSVLEEIGLT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003916
ORF Size 471 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003916.5
RefSeq Size 2283 bp
RefSeq ORF 474 bp
Locus ID 8905
UniProt ID P56377, Q549M9
Cytogenetics Xp22.2
Domains Clat_adaptor_s
Protein Families Druggable Genome
Protein Pathways Lysosome
MW 18.4 kDa
Summary Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as the small subunit of this complex and is a member of the adaptin protein family. Transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jan 2013
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Documents
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"NM_003916" in other vectors (7)
SKU Description Size Price
RC210030L1 Lenti ORF clone of Human adaptor-related protein complex 1, sigma 2 subunit (AP1S2), Myc-DDK-tagged 10 ug
$525.00
RC210030L2 Lenti ORF clone of Human adaptor-related protein complex 1, sigma 2 subunit (AP1S2), mGFP tagged 10 ug
$525.00
RC210030L3 Lenti ORF clone of Human adaptor-related protein complex 1, sigma 2 subunit (AP1S2), Myc-DDK-tagged 10 ug
$525.00
RC210030L4 Lenti ORF clone of Human adaptor-related protein complex 1, sigma 2 subunit (AP1S2), mGFP tagged 10 ug
$525.00
RG210030 AP1S2 (tGFP-tagged) - Human adaptor-related protein complex 1, sigma 2 subunit (AP1S2) 10 ug
$425.00
SC310658 AP1S2 (untagged)-Human adaptor-related protein complex 1, sigma 2 subunit (AP1S2) 10 ug
$165.00
SC321009 AP1S2 (untagged)-Human adaptor-related protein complex 1, sigma 2 subunit (AP1S2) 10 ug
$150.00

Plasmid Map for RC210030

Download sequence for

Cloning scheme for RC210030

Circular map for RC210030

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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