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XAF1 (NM_017523) Human Tagged ORF Clone

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SKU
RC209936
XAF1 (Myc-DDK-tagged)-Human XIAP associated factor 1 (XAF1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "XAF1 or NM_017523"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol XAF1
Synonyms BIRC4BP; HSXIAPAF1; XIAPAF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209936 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGGAGACTTCTCGGTGTGCAGGAACTGTAAAAGACATGTAGTCTCTGCCAACTTCACCCTCCATG
AGGCTTACTGCCTGCGGTTCCTGGTCCTGTGTCCGGAGTGTGAGGAGCCTGTCCCCAAGGAAACCATGGA
GGAGCACTGCAAGCTTGAGCACCAGCAGGTTGGGTGTACGATGTGTCAGCAGAGCATGCAGAAGTCCTCG
CTGGAGTTTCATAAGGCCAATGAGTGCCAGGAGCGCCCTGTTGAGTGTAAGTTCTGCAAACTGGACATGC
AGCTCAGCAAGCTGGAGCTCCACGAGTCCTACTGTGGCAGCCGGACAGAGCTCTGCCAAGGCTGTGGCCA
GTTCATCATGCACCGCATGCTCGCCCAGCACAGAGATGTCTGTCGCAGTGAACAGGCCCAGCTCGGGAAA
GGGGAAAGAATTTCAGCTCCTGAAAGGGAAATCTACTGTCATTATTGCAACCAAATGATTCCAGAAAATA
AGTATTTCCACCATATGGGTAAATGTTGTCCAGACTCAGAGTTTAAGAAACACTTTCCTGTTGGAAATCC
AGAAATTCTTCCTTCATCTCTTCCAAGTCAAGCTGCTGAAAATCAAACTTCCACGATGGAGAAAGATGTT
CGTCCAAAGACAAGAAGTATAAACAGATTTCCTCTTCATTCTGAAAGTTCATCAAAGAAAGCACCAAGAA
GCAAAAACAAAACCTTGGATCCACTTTTGATGTCAGAGCCCAAGCCCAGGACCAGCTCCCCTAGAGGAGA
TAAAGCAGCCTATGACATTCTGAGGAGATGTTCTCAGTGTGGCATCCTGCTTCCCCTGCCGATCCTAAAT
CAACATCAGGAGAAATGCCGGTGGTTAGCTTCATCAAAAGGAAAACAAGTGAGAAATTTCAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209936 protein sequence
Red=Cloning site Green=Tags(s)

MEGDFSVCRNCKRHVVSANFTLHEAYCLRFLVLCPECEEPVPKETMEEHCKLEHQQVGCTMCQQSMQKSS
LEFHKANECQERPVECKFCKLDMQLSKLELHESYCGSRTELCQGCGQFIMHRMLAQHRDVCRSEQAQLGK
GERISAPEREIYCHYCNQMIPENKYFHHMGKCCPDSEFKKHFPVGNPEILPSSLPSQAAENQTSTMEKDV
RPKTRSINRFPLHSESSSKKAPRSKNKTLDPLLMSEPKPRTSSPRGDKAAYDILRRCSQCGILLPLPILN
QHQEKCRWLASSKGKQVRNFS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017523
ORF Size 903 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017523.5
RefSeq Size 3638 bp
RefSeq ORF 906 bp
Locus ID 54739
UniProt ID Q6GPH4
Cytogenetics 17p13.1
Domains zf-TRAF
MW 34.6 kDa
Summary This gene encodes a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Feb 2012
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"NM_017523" in other vectors (7)
SKU Description Size Price
RC209936L1 Lenti ORF clone of Human XIAP associated factor 1 (XAF1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC209936L2 Lenti ORF clone of Human XIAP associated factor 1 (XAF1), transcript variant 1, mGFP tagged 10 ug
$600.00
RC209936L3 Lenti ORF clone of Human XIAP associated factor 1 (XAF1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC209936L4 Lenti ORF clone of Human XIAP associated factor 1 (XAF1), transcript variant 1, mGFP tagged 10 ug
$600.00
RG209936 XAF1 (tGFP-tagged) - Human XIAP associated factor 1 (XAF1), transcript variant 1 10 ug
$489.00 $500.00
SC114091 XAF1 (untagged)-Human XIAP associated factor 1 (XAF1), transcript variant 1 10 ug
$300.00
SC322019 XAF1 (untagged)-Human XIAP associated factor 1 (XAF1), transcript variant 1 10 ug
$300.00

Plasmid Map for RC209936

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209936
Circular map for RC209936

file-alt Citations

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