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XAF1 (NM_017523) Human Untagged Clone

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SKU
SC114091
XAF1 (untagged)-Human XIAP associated factor 1 (XAF1), transcript variant 1
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol XAF1
Synonyms BIRC4BP; HSXIAPAF1; XIAPAF1
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC114091 sequence for NM_017523 edited (data generated by NextGen Sequencing)
ATGGAAGGAGACTTCTCGGTGTGCAGGAACTGTAAAAGACATGTAGTCTCTGCCAACTTC
ACCCTCCATGAGGCTTACTGCCTGCGGTTCCTGGTCCTGTGTCCGGAGTGTGAGGAGCCT
GTCCCCAAGGAAACCATGGAGGAGCACTGCAAGCTTGAGCACCAGCAGGTTGGGTGTACG
ATGTGTCAGCAGAGCATGCAGAAGTCCTCGCTGGAGTTTCATAAGGCCAATGAGTGCCAG
GAGCGCCCTGTTGAGTGTAAGTTCTGCAAACTGGACATGCAGCTCAGCAAGCTGGAGCTC
CACGAGTCCTACTGTGGCAGCCGGACAGAGCTCTGCCAAGGCTGTGGCCAGTTCATCATG
CACCGCATGCTCGCCCAGCACAGAGATGTCTGTCGCAGTGAACAGGCCCAGCTCGGGAAA
GGGGAAAGAATTTCAGCTCCTGAAAGGGAAATCTACTGTCATTATTGCAACCAAATGATT
CCAGAAAATAAGTATTTCCACCATATGGGTAAATGTTGTCCAGACTCAGAGTTTAAGAAA
CACTTTCCTGTTGGAAATCCAGAAATTCTTCCTTCATCTCTTCCAAGTCAAGCTGCTGAA
AATCAAACTTCCACGATGGAGAAAGATGTTCGTCCAAAGACAAGAAGTATAAACAGATTT
CCTCTTCATTCTGAAAGTTCATCAAAGAAAGCACCAAGAAGCAAAAACAAAACCTTGGAT
CCACTTTTGATGTCAGAGCCCAAGCCCAGGACCAGCTCCCCTAGAGGAGATAAAGCAGCC
TATGACATTCTGAGGAGATGTTCTCAGTGTGGCATCCTGCTTCCCCTGCCGATCCTAAAT
CAACATCAGGAGAAATGCCGGTGGTTAGCTTCATCAAAAGGAAAACAAGTGAGAAATTTC
AGCTAG

Clone variation with respect to NM_017523.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_017523 unedited
NAAAATAATAACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCT
ATATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAG
CGGCCGCGAATTCGGCACGAGGAGAGAGCAGAACATGGAAGGAGACTTCTCGGTGTGCAG
GAACTGTAAAAGACATGTAGTCTCTGCCAACTTCACCCTCCATGAGGCTTACTGCCTGCG
GTTCCTGGTCCTGTGTCCGGAGTGTGAGGAGCCTGTCCCCAAGGAAACCATGGAGGAGCA
CTGCAAGCTTGAGCACCAGCAGGTTGGGTGTACGATGTGTCAGCAGAGCATGCAGAAGTC
CTCGCTGGAGTTTCATAAGGCCAATGAGTGCCAGGAGCGCCCTGTTGAGTGTAAGTTCTG
CAAACTGGACATGCAGCTCAGCAAGCTGGAGCTCCACGAGTCCTACTGTGGCAGCCGGAC
AGAGCTCTGCCAAGGCTGTGGCCAGTTCATCATGCACCGCATGCTCGCCCAGCACAGAGA
TGTCTGTCGCAGTGAACAGGCCCAGCTCGGGAAAGGGGAAAGAATTTCAGCTCCTGAAAG
GGAAATCTACTGTCATTATTGCAACCAAATGATTCCAGAAAATAAGTATTTCCACCATAT
GGGTAAATGTTGTCCAGACTCAGAGTTTAAGAAACACTTTCCTGTTGGAAATCCAGAAAT
TCTTCCTTCATCTCTTCCAAGTCAAGCTGCTGAAAATCAAACTTCCACGATGGAGAAAGA
TGTTCGTCCAAAGACCAGACGTATAAACAGATTTCCTCTTCATTCTGAAGNTCATCAAAG
AAAGCACCTAAGAGCACAACCAAACCTTGGATCCACTATTGATGTCAGAGCCCAGCCCAG
GACCAGCTTCTCTAGAGNAGATAAG
3' Read Nucleotide Sequence
>OriGene 3' read for NM_017523 unedited
GGAGCACTGAGGGCAGCGGGTCACAGGGCATGCCACCCGGGTATCTGTTGCAGAAAAAGC
TATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGGTTCAGGGGCA
CATGTGTAGGCTTGCTATATAGGTAAATTGCATGTCACCAGGGTTTGTTGTACAGATTAT
TTCATCATCCATATAAAAAGCATAGTACCAGATAGGTAGTTTTTTGATCCTCACCCTCCT
TCCATGCTCCGACCTCAGGTAGGCCCCAGTGTCTGCGGTTCCCTTCTTTGTATCCATGTG
TACTCAATGTTTAGCTTCTACTTATAAGTGAGAACATGTGGTATTTGGTTTTCTGTTTCT
GAGTGAGTTCATTTAGGATAATGGCTTCCAGCTCCATCCACGTTGCTGCACAGGACATGA
TCCCATTCTTTTTTATGGCTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTTT
TTTTTTTTTAAGGCAGAGTCTCGCTTGGTTGCCCAGCCGTGGAATTCAATGGAAAAACCC
CTGGTTTAATGGAAGCGGGAAGTTGCAGGTTCAAGCGAATCTCAAGGTTATGGCTATTCA
AGTAAATTGAATTAACGTGTGATCGGCCAACACACATGGCCAAATTTGCCTGTTGTTTAA
AAGAGACAGGAGTTTTCACCATGGTGGAACAGTCCCGGGCCCGNAAATTCCGGAATTGTT
AAGGAAAAAAATGAAAATAGCCGTTACATAAATATGTGGGAGAAAAAAAGAGAAGCCTGT
ATCGCCCTGGCCAAAAAATTTTCCTATATTCCTTTCAACCCCCGATGAACACTTAATTTT
GAAATGCAGAACATTCCGAAGCCCGACAATGAATAACAAATACAAT
Restriction Sites NotI-NotI |
ACCN NM_017523
Insert Size 3200 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017523.2, NP_059993.2
RefSeq Size 3638 bp
RefSeq ORF 906 bp
Locus ID 54739
UniProt ID Q6GPH4
Cytogenetics 17p13.1
Domains zf-TRAF
Summary This gene encodes a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Feb 2012
Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 7 encode the same isoform (1).
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Documents
Resources
"NM_017523" in other vectors (7)
SKU Description Size Price
RC209936 XAF1 (Myc-DDK-tagged)-Human XIAP associated factor 1 (XAF1), transcript variant 1 10 ug
$300.00
RC209936L1 Lenti ORF clone of Human XIAP associated factor 1 (XAF1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC209936L2 Lenti ORF clone of Human XIAP associated factor 1 (XAF1), transcript variant 1, mGFP tagged 10 ug
$600.00
RC209936L3 Lenti ORF clone of Human XIAP associated factor 1 (XAF1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC209936L4 Lenti ORF clone of Human XIAP associated factor 1 (XAF1), transcript variant 1, mGFP tagged 10 ug
$600.00
RG209936 XAF1 (tGFP-tagged) - Human XIAP associated factor 1 (XAF1), transcript variant 1 10 ug
$489.00 $500.00
SC322019 XAF1 (untagged)-Human XIAP associated factor 1 (XAF1), transcript variant 1 10 ug
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file-alt Citations

Wild-type PABPN1 is anti-apoptotic and reduces toxicity of the oculopharyngeal muscular dystrophy mutation
Davies, Sarkar, Rubinsztein
Hum Mol Genet (2008) 17 (8), 1097-108 DOI: 10.1093/hmg/ddm382
Cited by 47
AI Summary: OriGene products contributed to this study by providing the PABPN1 cDNA clone (OriGene, SC319661) and shRNA plasmids targeting PABPN1 (OriGene, TL311386), which were used to overexpress and knock down PABPN1, respectively, enabling the authors to investigate the protective role of wild-type PABPN1 against mutant toxicity and apoptosis in cell and mouse models of OPMD.
Showing 1-1 of 1 papers.
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