SPIN2A (NM_019003) Human Tagged ORF Clone

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SKU
RC209934
SPIN2A (Myc-DDK-tagged)-Human spindlin family, member 2A (SPIN2A)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPIN2A
Synonyms dJ323P24.1; DXF34; SPIN2; TDRD25
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209934 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGACCCCCAACGCACAGGAAGCCGAAGGGCAACAAACCAGGGCAGCTGCAGGACGGGCCACTGGGT
CTGCAAACATGACAAAGAAAAAAGTCTCCCAAAAGAAGCAGAGAGGCAGACCTTCATCCCAACCCTGCAG
GAACATCGTGGGCTGCAGAATTTCTCATGGATGGAAGGAAGGAGATGAGCCCATCACGCAGTGGAAAGGA
ACCGTTCTGGATCAGGTGCCTATAAATCCCTCTCTTTATCTGGTGAAATATGATGGAATTGACTGTGTCT
ATGGACTGGAACTTCACAGAGATGAAAGGGTTTTGTCTCTTAAAATTCTTTCTGACAGGGTGGCATCATC
TCACATTAGTGATGCCAACCTTGCAAATACCATAATTGGCAAAGCAGTGGAACACATGTTTGAGGGAGAG
CATGGTTCTAAGGATGAATGGAGGGGGATGGTCTTAGCTCAAGCACCTATCATGAAAGCCTGGTTTTATA
TTACCTATGAGAAAGATCCTGTCTTGTACATGTACCAGCTTCTAGATGATTATAAGGAAGGTGACCTCCG
CATCATGCCAGAATCCAGTGAGTCTCCTCCAACAGAGAGGGAGCCAGGAGGAGTTGTAGATGGCCTAATA
GGTAAGCATGTGGAATATACCAAAGAAGATGGCTCCAAAAGGATCGGCATGGTCATTCACCAAGTGGAAA
CCAAACCCTCTGTGTATTTCATCAAGTTTGATGATGATTTCCATATCTATGTCTACGATTTGGTGAAAAA
GTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC209934 protein sequence
Red=Cloning site Green=Tags(s)

MKTPNAQEAEGQQTRAAAGRATGSANMTKKKVSQKKQRGRPSSQPCRNIVGCRISHGWKEGDEPITQWKG
TVLDQVPINPSLYLVKYDGIDCVYGLELHRDERVLSLKILSDRVASSHISDANLANTIIGKAVEHMFEGE
HGSKDEWRGMVLAQAPIMKAWFYITYEKDPVLYMYQLLDDYKEGDLRIMPESSESPPTEREPGGVVDGLI
GKHVEYTKEDGSKRIGMVIHQVETKPSVYFIKFDDDFHIYVYDLVKKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019003
ORF Size 774 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019003.2, NP_061876.2
RefSeq Size 1351 bp
RefSeq ORF 777 bp
Locus ID 54466
UniProt ID Q99865, A0A024R9W1
Cytogenetics Xp11.21
Domains Spin-Ssty
MW 29.1 kDa
Summary This gene encodes one of three members of the DXF34 gene family, located in a 100-kb region of chromosome Xp11.21. provided by RefSeq, Nov 2009
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Documents
Resources
"NM_019003" in other vectors (5)
SKU Description Size Price
RC209934L3 Lenti ORF clone of Human spindlin family, member 2A (SPIN2A), Myc-DDK-tagged 10 ug
$600.00
RC209934L4 Lenti ORF clone of Human spindlin family, member 2A (SPIN2A), mGFP tagged 10 ug
$600.00
RG209934 SPIN2A (tGFP-tagged) - Human spindlin family, member 2A (SPIN2A) 10 ug
$489.00 $500.00
SC108740 SPIN2A (untagged)-Human spindlin family, member 2A (SPIN2A) 10 ug
$300.00
SC321961 SPIN2A (untagged)-Human spindlin family, member 2A (SPIN2A) 10 ug
$300.00

Plasmid Map for RC209934

Download sequence for

Cloning scheme for RC209934

Circular map for RC209934

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