CDK5RAP3 (NM_176096) Human Tagged ORF Clone
SKU
RC209901
CDK5RAP3 (Myc-DDK-tagged)-Human CDK5 regulatory subunit associated protein 3 (CDK5RAP3)
$471.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CDK5RAP3 |
| Synonyms | C53; HSF-27; IC53; LZAP; MST016; OK/SW-cl.114; PP1553 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_176096 |
| ORF Size | 1518 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_176096.3 |
| RefSeq Size | 1996 bp |
| RefSeq ORF | 1521 bp |
| Locus ID | 80279 |
| UniProt ID | Q96JB5 |
| Cytogenetics | 17q21.32 |
| MW | 56.9 kDa |
| Summary | This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms. provided by RefSeq, May 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_176096" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209901L1 | Lenti ORF clone of Human CDK5 regulatory subunit associated protein 3 (CDK5RAP3), Myc-DDK-tagged | 10 ug |
$771.00
|
|
| RC209901L2 | Lenti ORF clone of Human CDK5 regulatory subunit associated protein 3 (CDK5RAP3), mGFP tagged | 10 ug |
$771.00
|
|
| RC209901L3 | Lenti ORF clone of Human CDK5 regulatory subunit associated protein 3 (CDK5RAP3), Myc-DDK-tagged | 10 ug |
$771.00
|
|
| RC209901L4 | Lenti ORF clone of Human CDK5 regulatory subunit associated protein 3 (CDK5RAP3), mGFP tagged | 10 ug |
$771.00
|
|
| RG209901 | CDK5RAP3 (tGFP-tagged) - Human CDK5 regulatory subunit associated protein 3 (CDK5RAP3) | 10 ug |
$489.00
$671.00
|
|
| SC110641 | CDK5RAP3 (untagged)-Human CDK5 regulatory subunit associated protein 3 (CDK5RAP3) | 10 ug |
$472.00
|
|
Citations
C53 Interacting with UFM1-Protein Ligase 1 Regulates Microtubule Nucleation in Response to ER Stress
Klebanovych, Vinopal, Dráberová et al
Cells (2022) 11 (3) DOI: 10.3390/cells11030555
Klebanovych, Vinopal, Dráberová et al
Cells (2022) 11 (3) DOI: 10.3390/cells11030555
Cited by 21
AI Summary: OriGene products, specifically purified C-terminally FLAG-tagged C53 and nucleophosmin, were used in this study to investigate protein interactions and mechanisms underlying the regulation of microtubule nucleation in response to ER stress.
Snippet: bunit-associated protein 3 (also known as CDK5RAP3, LZAP;hereafter denoted as C53) exerts multiple roles in regulating the cell cycle, DNA damage response, cell survival, cell adherence/invasion, tumorigenesis, and metastasis [21,22,23,24]. Moreover, C53 is also involved in UPR [19]. …
Nuclear γ-tubulin associates with nucleoli and interacts with tumor suppressor protein C53
Hořejší, Vinopal, Sládková et al
J. Cell. Physiol. (2012) 227 (1), 367-82 DOI: 10.1002/jcp.22772
Hořejší, Vinopal, Sládková et al
J. Cell. Physiol. (2012) 227 (1), 367-82 DOI: 10.1002/jcp.22772
Cited by 56
AI Summary: In this study, OriGene’s human C53 cDNA clone (#RC209901) was used to generate tagged C53 constructs, enabling the investigation of interactions between γ-tubulin and the tumor suppressor protein C53.
Snippet: tagged C53 (pC53-EGFP) was prepared by PCR amplification of full length cDNA of human C53 clone (#RC209901;Origene) using forward ... dissolved in 2x Laemmli sample buffer and separated on 8% SDS-PAGE (50 µl sample/well) using Multigel-Long electrophoretic system
Showing 1-2 of 2 papers.
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