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CPSF2 (NM_017437) Human Tagged ORF Clone

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SKU
RC209857
CPSF2 (Myc-DDK-tagged)-Human cleavage and polyadenylation specific factor 2, 100kDa (CPSF2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $716.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CPSF2
Synonyms CPSF100
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209857 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGTCTATTATCAAATTAACTACCCTTTCTGGGGTCCAAGAAGAATCTGCCCTTTGCTATCTTCTCC
AAGTTGATGAGTTTAGATTTTTATTGGACTGTGGCTGGGATGAGCACTTTTCTATGGATATTATTGATTC
CCTGAGGAAGCATGTTCACCAGATTGATGCAGTGCTGTTGTCTCACCCTGATCCTCTCCACCTTGGTGCC
CTCCCGTATGCTGTCGGAAAGTTGGGTCTGAACTGTGCTATCTATGCAACCATTCCTGTTTATAAAATGG
GACAGATGTTCATGTATGATCTTTATCAGTCTCGACACAATACAGAAGATTTTACACTCTTTACATTAGA
TGATGTGGATGCAGCCTTTGATAAAATACAGCAGCTAAAATTCTCTCAGATTGTGAATTTGAAAGGTAAA
GGACATGGCCTGTCTATCACACCTCTGCCAGCTGGTCATATGATAGGTGGAACAATATGGAAAATAGTCA
AAGATGGAGAAGAAGAAATTGTTTATGCAGTTGACTTCAACCACAAGAGGGAGATCCATTTAAATGGATG
TTCCCTGGAAATGCTAAGCAGGCCTTCCCTACTTATCACAGATTCATTCAATGCTACATATGTACAGCCT
AGAAGAAAACAGAGAGATGAGCAGCTTCTGACAAATGTCCTGGAAACACTTCGAGGTGATGGAAATGTGT
TAATAGCAGTGGACACAGCAGGCAGAGTTTTGGAACTTGCTCAACTTCTTGATCAGATTTGGAGGACTAA
AGATGCAGGATTGGGTGTTTACTCATTGGCACTCCTAAATAATGTCAGTTACAATGTGGTGGAGTTTTCT
AAGTCCCAGGTAGAATGGATGAGTGGTAAATTGATGAGATGTTTTGAAGACAAAAGAAATAATCCGTTTC
AGTTTCGCCATCTCTCTTTATGTCATGGTCTTTCTGACTTGGCCCGTGTACCTAGCCCTAAAGTTGTACT
TGCCAGCCAACCTGACCTGGAATGCGGATTTTCAAGGGATCTCTTTATTCAGTGGTGTCAGGACCCTAAA
AACTCAATCATTCTAACCTACAGAACTACTCCTGGGACTTTAGCACGTTTCCTAATTGATAATCCTTCTG
AAAAAATTACAGAAATAGAGTTGAGGAAACGTGTGAAGCTTGAAGGGAAAGAACTTGAAGAATACTTGGA
AAAAGAGAAACTAAAGAAAGAAGCTGCCAAAAAGCTTGAGCAGTCAAAAGAGGCAGATATAGATTCCAGT
GATGAGAGTGATATTGAGGAAGATATTGACCAGCCATCAGCTCATAAGACGAAGCATGACTTGATGATGA
AAGGTGAAGGCAGTCGTAAAGGAAGTTTTTTCAAACAGGCAAAAAAGTCCTATCCTATGTTTCCTGCCCC
AGAAGAAAGAATTAAATGGGATGAATATGGAGAGATTATCAAACCAGAGGATTTCTTAGTGCCAGAGCTT
CAAGCTACTGAAGAAGAAAAAAGCAAATTAGAATCTGGTTTGACAAATGGAGATGAACCTATGGATCAGG
ATTTATCTGATGTTCCTACTAAATGTATTTCTACAACAGAGTCTATTGAAATAAAAGCCCGGGTTACCTA
CATAGACTATGAAGGACGCTCTGATGGGGATTCCATTAAAAAAATCATTAATCAGATGAAACCACGACAG
TTGATCATCGTCCATGGCCCACCAGAGGCCAGTCAAGATCTGGCAGAGTGCTGTCGCGCCTTTGGTGGGA
AAGATATTAAAGTGTACATGCCAAAGCTACATGAAACAGTTGATGCCACTAGTGAAACTCACATCTACCA
GGTGAGGTTAAAAGACTCACTTGTCAGCTCTCTTCAGTTTTGTAAGGCAAAAGATGCTGAATTAGCTTGG
ATAGATGGTGTCTTAGATATGAGAGTTTCCAAAGTGGACACAGGGGTTATTTTAGAAGAAGGAGAACTAA
GGGATGATGGAGAAGACTCAGAGATGCAAGTGGAAGCTCCCTCAGATTCTAGCGTTATAGCACAACAAAA
GGCCATGAAAAGTTTGTTCGGAGATGATGAAAAAGAAACAGGTGAAGAAAGTGAGATCATTCCTACTTTG
GAACCCTTGCCACCTCATGAGGTTCCTGGACATCAGTCAGTTTTTATGAATGAACCAAGGCTGTCAGACT
TCAAGCAAGTTCTCTTACGGGAGGGAATTCAAGCTGAATTTGTAGGAGGTGTACTTGTTTGCAACAATCA
AGTAGCAGTCCGCAGAACGGAAACTGGACGCATTGGATTAGAAGGCTGCCTTTGTCAAGATTTTTATAGG
ATAAGAGACCTTTTATATGAACAATATGCCATTGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209857 protein sequence
Red=Cloning site Green=Tags(s)

MTSIIKLTTLSGVQEESALCYLLQVDEFRFLLDCGWDEHFSMDIIDSLRKHVHQIDAVLLSHPDPLHLGA
LPYAVGKLGLNCAIYATIPVYKMGQMFMYDLYQSRHNTEDFTLFTLDDVDAAFDKIQQLKFSQIVNLKGK
GHGLSITPLPAGHMIGGTIWKIVKDGEEEIVYAVDFNHKREIHLNGCSLEMLSRPSLLITDSFNATYVQP
RRKQRDEQLLTNVLETLRGDGNVLIAVDTAGRVLELAQLLDQIWRTKDAGLGVYSLALLNNVSYNVVEFS
KSQVEWMSGKLMRCFEDKRNNPFQFRHLSLCHGLSDLARVPSPKVVLASQPDLECGFSRDLFIQWCQDPK
NSIILTYRTTPGTLARFLIDNPSEKITEIELRKRVKLEGKELEEYLEKEKLKKEAAKKLEQSKEADIDSS
DESDIEEDIDQPSAHKTKHDLMMKGEGSRKGSFFKQAKKSYPMFPAPEERIKWDEYGEIIKPEDFLVPEL
QATEEEKSKLESGLTNGDEPMDQDLSDVPTKCISTTESIEIKARVTYIDYEGRSDGDSIKKIINQMKPRQ
LIIVHGPPEASQDLAECCRAFGGKDIKVYMPKLHETVDATSETHIYQVRLKDSLVSSLQFCKAKDAELAW
IDGVLDMRVSKVDTGVILEEGELRDDGEDSEMQVEAPSDSSVIAQQKAMKSLFGDDEKETGEESEIIPTL
EPLPPHEVPGHQSVFMNEPRLSDFKQVLLREGIQAEFVGGVLVCNNQVAVRRTETGRIGLEGCLCQDFYR
IRDLLYEQYAIV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017437
ORF Size 2346 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017437.3
RefSeq Size 5072 bp
RefSeq ORF 2349 bp
Locus ID 53981
UniProt ID Q9P2I0
Cytogenetics 14q32.12
MW 88.5 kDa
Summary Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing.UniProtKB/Swiss-Prot Function
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Documents
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"NM_017437" in other vectors (4)
SKU Description Size Price
RC209857L3 Lenti-ORF clone of CPSF2 (Myc-DDK-tagged)-Human cleavage and polyadenylation specific factor 2, 100kDa (CPSF2) 10 ug
$1,016.00
RC209857L4 Lenti-ORF clone of CPSF2 (mGFP-tagged)-Human cleavage and polyadenylation specific factor 2, 100kDa (CPSF2) 10 ug
$1,016.00
RG209857 CPSF2 (tGFP-tagged) - Human cleavage and polyadenylation specific factor 2, 100kDa (CPSF2) 10 ug
$789.00 $916.00
SC107581 CPSF2 (untagged)-Human cleavage and polyadenylation specific factor 2, 100kDa (CPSF2) 10 ug
$717.00

Plasmid Map for RC209857

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209857
Circular map for RC209857

file-alt Citations

JTE-607, a multiple cytokine production inhibitor, targets CPSF3 and inhibits pre-mRNA processing
Kakegawa, Sakane, Suzuki et al
Biochem. Biophys. Res. Commun. (2019) 518 (1), 32-37 DOI: 10.1016/j.bbrc.2019.08.004
AI Summary: OriGene products contributed to this study by providing full-length cDNA molecules encoding human CPSF2, CPSF3, and symplekin, which were used to investigate the molecular targets and mechanism of action of JTE-607.
Snippet: The compounds were dissolved in dimethylsulfoxide (DMSO) and diluted with cell culture medium. Full length cDNA molecules encoding human CPSF2, CPSF3, or symplekin were purchased from OriGene Technologies, Inc. (Rockville, MD). 2.2
Showing 1-1 of 1 papers.
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