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CD62P (SELP) (NM_003005) Human Tagged ORF Clone

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SKU
RC209822
SELP (Myc-DDK-tagged)-Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $759.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CD62P
Synonyms CD62; CD62P; GMP140; GRMP; LECAM3; PADGEM; PSEL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209822 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAACTGCCAAATAGCCATCTTGTACCAGAGATTCCAGAGAGTGGTCTTTGGAATTTCCCAACTCC
TTTGCTTCAGTGCCCTGATCTCTGAACTAACAAACCAGAAAGAAGTGGCAGCATGGACTTATCATTACAG
CACAAAAGCATACTCATGGAATATTTCCCGTAAATACTGCCAGAATCGCTACACAGACTTAGTGGCCATC
CAGAATAAAAATGAAATTGATTACCTCAATAAGGTCCTACCCTACTACAGCTCCTACTACTGGATTGGGA
TCCGAAAGAACAATAAGACATGGACATGGGTGGGAACCAAAAAGGCTCTCACCAACGAGGCTGAGAACTG
GGCTGATAATGAACCTAACAACAAAAGGAACAACGAGGACTGCGTGGAGATATACATCAAGAGTCCGTCA
GCCCCTGGCAAGTGGAATGATGAGCACTGCTTGAAGAAAAAGCACGCATTGTGTTACACAGCCTCCTGCC
AGGACATGTCCTGCAGCAAACAAGGAGAGTGCCTCGAGACCATCGGGAACTACACCTGCTCCTGTTACCC
TGGATTCTATGGGCCAGAATGTGAATACGTGAGAGAGTGTGGAGAACTTGAGCTCCCTCAACACGTGCTC
ATGAACTGCAGCCACCCTCTGGGAAACTTCTCTTTTAACTCGCAGTGCAGCTTCCACTGCACTGACGGGT
ACCAAGTAAATGGGCCCAGCAAGCTGGAATGCTTGGCTTCTGGAATCTGGACAAATAAGCCTCCACAGTG
TTTAGCTGCCCAGTGCCCACCCCTGAAGATTCCTGAACGAGGAAACATGACCTGCCTTCATTCTGCAAAA
GCATTCCAGCATCAGTCTAGCTGCAGCTTCAGTTGTGAAGAGGGATTTGCATTAGTTGGACCGGAAGTGG
TGCAATGCACAGCCTCGGGGGTATGGACAGCCCCAGCCCCAGTGTGTAAAGCTGTGCAGTGTCAGCACCT
GGAAGCCCCCAGTGAAGGAACCATGGACTGTGTTCATCCGCTCACTGCTTTTGCCTATGGCTCCAGCTGT
AAATTTGAGTGCCAGCCCGGCTACAGAGTGAGGGGCTTGGACATGCTCCGCTGCATTGACTCTGGACACT
GGTCTGCACCCTTGCCAACCTGTGAGGCTATTTCGTGTGAGCCGCTGGAGAGTCCTGTCCACGGAAGCAT
GGATTGCTCTCCATCCTTGAGAGCGTTTCAGTATGACACCAACTGTAGCTTCCGCTGTGCTGAAGGTTTC
ATGCTGAGAGGAGCCGATATAGTTCGGTGTGATAACTTGGGACAGTGGACAGCACCAGCCCCAGTCTGTC
AAGCTTTGCAGTGCCAGGATCTCCCAGTTCCAAATGAGGCCCGGGTGAACTGCTCCCACCCCTTCGGTGC
CTTTAGGTACCAGTCAGTCTGCAGCTTCACCTGCAATGAAGGCTTGCTCCTGGTGGGAGCAAGTGTGCTA
CAGTGCTTGGCTACTGGAAACTGGAATTCTGTTCCTCCAGAATGCCAAGCCATTCCCTGCACACCTTTGC
TAAGCCCTCAGAATGGAACAATGACCTGTGTTCGACCTCTTGGAAGTTCCAGTTATAAATCCACATGTCA
ATTCATCTGTGACGAGGGATATTCTTTGTCTGGACCAGAAAGATTGGATTGTACTCGATCGGGACGCTGG
ACAGACTCCCCACCAATGTGTGAAGCCATCAAGTGCCCAGAACTCTTTGCCCCAGAGCAGGGCAGCCTGG
ATTGTTCTGACACTCGTGGAGAATTCAATGTTGGCTCCACCTGCCATTTCTCTTGTAACAACGGCTTTAA
GCTGGAGGGGCCCAATAATGTGGAATGCACAACTTCTGGAAGATGGTCAGCTACTCCACCAACCTGCAAA
GGCATAGCATCACTTCCTACTCCAGGGGTGCAATGTCCAGCCCTCACCACTCCTGGGCAGGGAACCATGT
ACTGTAGGCATCATCCGGGAACCTTTGGTTTTAATACCACTTGTTACTTTGGCTGCAACGCTGGATTCAC
ACTCATAGGAGACAGCACTCTCAGCTGCAGACCTTCAGGACAATGGACAGCAGTAACTCCAGCATGCAGA
GCTGTGAAATGCTCAGAACTACATGTTAATAAGCCAATAGCGATGAACTGCTCCAACCTCTGGGGAAACT
TCAGTTATGGATCAATCTGCTCTTTCCATTGTCTAGAGGGCCAGTTACTTAATGGCTCTGCACAAACAGC
ATGCCAAGAGAATGGCCACTGGTCAACTACCGTGCCAACCTGCCAAGGACCATTGACTATCCAGGAAGCC
CTGACTTACTTTGGTGGAGCGGTGGCTTCTACGATAGGTTTGATAATGGGTGGGACGCTCCTGGCTTTGC
TAAGAAAGCGTTTCAGACAAAAAGATGATGGGAAATGCCCCTTGAATCCTCACAGCCACCTAGGAACATA
TGGAGTTTTTACAAACGCTGCATTTGACCCGAGTCCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209822 protein sequence
Red=Cloning site Green=Tags(s)

MANCQIAILYQRFQRVVFGISQLLCFSALISELTNQKEVAAWTYHYSTKAYSWNISRKYCQNRYTDLVAI
QNKNEIDYLNKVLPYYSSYYWIGIRKNNKTWTWVGTKKALTNEAENWADNEPNNKRNNEDCVEIYIKSPS
APGKWNDEHCLKKKHALCYTASCQDMSCSKQGECLETIGNYTCSCYPGFYGPECEYVRECGELELPQHVL
MNCSHPLGNFSFNSQCSFHCTDGYQVNGPSKLECLASGIWTNKPPQCLAAQCPPLKIPERGNMTCLHSAK
AFQHQSSCSFSCEEGFALVGPEVVQCTASGVWTAPAPVCKAVQCQHLEAPSEGTMDCVHPLTAFAYGSSC
KFECQPGYRVRGLDMLRCIDSGHWSAPLPTCEAISCEPLESPVHGSMDCSPSLRAFQYDTNCSFRCAEGF
MLRGADIVRCDNLGQWTAPAPVCQALQCQDLPVPNEARVNCSHPFGAFRYQSVCSFTCNEGLLLVGASVL
QCLATGNWNSVPPECQAIPCTPLLSPQNGTMTCVRPLGSSSYKSTCQFICDEGYSLSGPERLDCTRSGRW
TDSPPMCEAIKCPELFAPEQGSLDCSDTRGEFNVGSTCHFSCNNGFKLEGPNNVECTTSGRWSATPPTCK
GIASLPTPGVQCPALTTPGQGTMYCRHHPGTFGFNTTCYFGCNAGFTLIGDSTLSCRPSGQWTAVTPACR
AVKCSELHVNKPIAMNCSNLWGNFSYGSICSFHCLEGQLLNGSAQTACQENGHWSTTVPTCQGPLTIQEA
LTYFGGAVASTIGLIMGGTLLALLRKRFRQKDDGKCPLNPHSHLGTYGVFTNAAFDPSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003005
ORF Size 2487 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003005.4
RefSeq Size 3185 bp
RefSeq ORF 2493 bp
Locus ID 6403
UniProt ID P16109, A0A024R8Y9, Q6NUL9
Cytogenetics 1q24.2
Domains CCP, CLECT, EGF
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Cell adhesion molecules (CAMs)
MW 90.8 kDa
Summary This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. provided by RefSeq, Jul 2008
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"NM_003005" in other vectors (6)
SKU Description Size Price
RC209822L1 Lenti ORF clone of Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP), Myc-DDK-tagged 10 ug
$1,059.00
RC209822L2 Lenti ORF clone of Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP), mGFP tagged 10 ug
$1,059.00
RC209822L3 Lenti ORF clone of Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP), Myc-DDK-tagged 10 ug
$1,059.00
RC209822L4 Lenti ORF clone of Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP), mGFP tagged 10 ug
$1,059.00
RG209822 SELP (tGFP-tagged) - Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP) 10 ug
$789.00 $959.00
SC118236 SELP (untagged)-Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP) 10 ug
$761.00

Plasmid Map for RC209822

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209822
Circular map for RC209822

file-alt Citations

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