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CD62P (SELP) (NM_003005) Human Untagged Clone

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SKU
SC118236
SELP (untagged)-Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP)
  $761.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CD62P
Synonyms CD62; CD62P; GMP140; GRMP; LECAM3; PADGEM; PSEL
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_003005, the custom clone sequence may differ by one or more nucleotides


ATGGCCAACTGCCAAATAGCCATCTTGTACCAGAGATTCCAGAGAGTGGTCTTTGGAATTTCCCAACTCC
TTTGCTTCAGTGCCCTGATCTCTGAACTAACAAACCAGAAAGAAGTGGCAGCATGGACTTATCATTACAG
CACAAAAGCATACTCATGGAATATTTCCCGTAAATACTGCCAGAATCGCTACACAGACTTAGTGGCCATC
CAGAATAAAAATGAAATTGATTACCTCAATAAGGTCCTACCCTACTACAGCTCCTACTACTGGATTGGGA
TCCGAAAGAACAATAAGACATGGACATGGGTGGGAACCAAAAAGGCTCTCACCAACGAGGCTGAGAACTG
GGCTGATAATGAACCTAACAACAAAAGGAACAACGAGGACTGCGTGGAGATATACATCAAGAGTCCGTCA
GCCCCTGGCAAGTGGAATGATGAGCACTGCTTGAAGAAAAAGCACGCATTGTGTTACACAGCCTCCTGCC
AGGACATGTCCTGCAGCAAACAAGGAGAGTGCCTCGAGACCATCGGGAACTACACCTGCTCCTGTTACCC
TGGATTCTATGGGCCAGAATGTGAATACGTGAGAGAGTGTGGAGAACTTGAGCTCCCTCAACACGTGCTC
ATGAACTGCAGCCACCCTCTGGGAAACTTCTCTTTTAACTCGCAGTGCAGCTTCCACTGCACTGACGGGT
ACCAAGTAAATGGGCCCAGCAAGCTGGAATGCTTGGCTTCTGGAATCTGGACAAATAAGCCTCCACAGTG
TTTAGCTGCCCAGTGCCCACCCCTGAAGATTCCTGAACGAGGAAACATGACCTGCCTTCATTCTGCAAAA
GCATTCCAGCATCAGTCTAGCTGCAGCTTCAGTTGTGAAGAGGGATTTGCATTAGTTGGACCGGAAGTGG
TGCAATGCACAGCCTCGGGGGTATGGACAGCCCCAGCCCCAGTGTGTAAAGCTGTGCAGTGTCAGCACCT
GGAAGCCCCCAGTGAAGGAACCATGGACTGTGTTCATCCGCTCACTGCTTTTGCCTATGGCTCCAGCTGT
AAATTTGAGTGCCAGCCCGGCTACAGAGTGAGGGGCTTGGACATGCTCCGCTGCATTGACTCTGGACACT
GGTCTGCACCCTTGCCAACCTGTGAGGCTATTTCGTGTGAGCCGCTGGAGAGTCCTGTCCACGGAAGCAT
GGATTGCTCTCCATCCTTGAGAGCGTTTCAGTATGACACCAACTGTAGCTTCCGCTGTGCTGAAGGTTTC
ATGCTGAGAGGAGCCGATATAGTTCGGTGTGATAACTTGGGACAGTGGACAGCACCAGCCCCAGTCTGTC
AAGCTTTGCAGTGCCAGGATCTCCCAGTTCCAAATGAGGCCCGGGTGAACTGCTCCCACCCCTTCGGTGC
CTTTAGGTACCAGTCAGTCTGCAGCTTCACCTGCAATGAAGGCTTGCTCCTGGTGGGAGCAAGTGTGCTA
CAGTGCTTGGCTACTGGAAACTGGAATTCTGTTCCTCCAGAATGCCAAGCCATTCCCTGCACACCTTTGC
TAAGCCCTCAGAATGGAACAATGACCTGTGTTCAACCTCTTGGAAGTTCCAGTTATAAATCCACATGTCA
ATTCATCTGTGACGAGGGATATTCTTTGTCTGGACCAGAAAGATTGGATTGTACTCGATCGGGACGCTGG
ACAGACTCCCCACCAATGTGTGAAGCCATCAAGTGCCCAGAACTCTTTGCCCCAGAGCAGGGCAGCCTGG
ATTGTTCTGACACTCGTGGAGAATTCAATGTTGGCTCCACCTGCCATTTCTCTTGTGACAACGGCTTTAA
GCTGGAGGGGCCCAATAATGTGGAATGCACAACTTCTGGAAGATGGTCAGCTACTCCACCAACCTGCAAA
GGCATAGCATCACTTCCTACTCCAGGGGTGCAATGTCCAGCCCTCACCACTCCTGGGCAGGGAACCATGT
ACTGTAGGCATCATCCGGGAACCTTTGGTTTTAATACCACTTGTTACTTTGGCTGCAACGCTGGATTCAC
ACTCATAGGAGACAGCACTCTCAGCTGCAGACCTTCAGGACAATGGACAGCAGTAACTCCAGCATGCAGA
GCTGTGAAATGCTCAGAACTACATGTTAATAAGCCAATAGCGATGAACTGCTCCAACCTCTGGGGAAACT
TCAGTTATGGATCAATCTGCTCTTTCCATTGTCTAGAGGGCCAGTTACTTAATGGCTCTGCACAAACAGC
ATGCCAAGAGAATGGCCACTGGTCAACTACCGTGCCAACCTGCCAAGCAGGACCATTGACTATCCAGGAA
GCCCTGACTTACTTTGGTGGAGCGGTGGCTTCTACGATAGGTCTGATAATGGGTGGGACGCTCCTGGCTT
TGCTAAGAAAGCGTTTCAGACAAAAAGATGATGGGAAATGCCCCTTGAATCCTCACAGCCACCTAGGAAC
ATATGGAGTTTTTACAAACGCTGCATTTGACCCGAGTCCTTAA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_003005 unedited
GGGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGTCACAG
AGGAGATGGCCAACTGCCAAATAGCCATCTTGTACCAGAGATTCCAGAGAGTGGTCTTTG
GAATTTCCCAACTCCTTTGCTTCAGTGCCCTGATCTCTGAACTAACAAACCAGAAAGAAG
TGGCAGCATGGACTTATCATTACAGCACAAAAGCATACTCATGGAATATTTCCCGTAAAT
ACTGCCAGAATCGCTACACAGACTTAGTGGCCATCCAGAATAAAAATGAAATTGATTACC
TCAATAAGGTCCTACCCTACTACAGCTCCTACTACTGGATTGGGATCCGAAAGAACAATA
AGACATGGACATGGGTGGGAACCAAAAAGGCTCTCACCAACGAGGCTGAGAACTGGGCTG
ATAATGAACCTAACAACAAAAGGAACAACGAGGACTGCGTGGAGATATACATCAAGAGTC
CGTCAGCCCCTGGCAAGTGGAATGATGAGCACTGCTTGAAGAAAAAGCACGCATTGTGTT
ACACAGCCTCCTGCCAGGACATGTCCTGCAGCAAACAAGGAGAGTGCCTCGAGACCATCG
GGAACTACACCTGCTCCTGTTACCCTGGATTCTATGGGCCAGAATGTGAATACGTGAGAG
AGTGTGGAGAACTTGAGCTCCCTCAACACGTGCTCATGAACTGCAGCCACCCTCTGGGAA
ACTTCTCTTTTAACTCGCAGTGCAGCTTCCACTGCACTGACGGGTACCAAGTAAATGGGC
CCAGCAAGCTGGAATGCTTGGCTTCTGGGATCTGGACAAATAAGCCCCACAGTGTTTAGC
TGCCCAGTGCCCACCCCTGAAGATTCCCTGACGAGAAAACATGACCTGCCTTCATTCTGC
AAAAGC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_003005 unedited
CGAGGCCGCAATTTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTGGGGAAAAATCTTTTAAATACCCCTTTGAAAATTGGGCTTTGGGGC
ATTTGAGGGACAAGGACTGGGGGGAAGAAAGGCCACCGGGACCTGGGGGCCTTTAACTTG
AATTTTGGGCCTTTGGAACCCCTGGGGCCCTTCAAAAGAATCTTTGGAAACCCCAAAACA
AAAGGCCAAAAAGGCCCATTAACAGCAACCACTGGAGAATCCAAAAAAAGCTTCAGGAAA
TTCTTTCAATTAAAAAAGCCCCCCGGTTTTCAACAAAAGGGAAAAAACCCTTCTTTAATC
AAAAAATAAAAAGGGATTTTTATTTTTCCACAAGCTGGGCTTTTTGGGAAACAAAAGCTC
CAACCTCTTTGGGTCACCGGGATTTCAAGGAACAGGGGAGTCAAAACACCAAGACCACCG
GAGAAACATTTGCCCCTGGGTTCCGGGGGTTGGGTTACAAGAAAAAAAAAAGCGGGACAC
GAAAGGGGGGGAAGGTCGAAAATTCGAAATGGTCCACCATCCCCAACCTCAGAAAAAGGG
GTGGGTCCCAACGGGCCCACACAGCGGGGCCACAAGTTAATCAAAGGAAACTCCCGTGTT
TGATATAATGGGGGGTAAGGGAACCCGCCGCAAAAGGCCTCTTATGTTAGTCCCCCGGTG
AATGGGAAACCTGGGTGGTCCAAAACAGGACATATTGGTGGGCGGCCGGAGGGACCACCC
CAAAAGGACACGGTTTTTTAAGGGCCCGGGGCAACAGCCCCGCTTTGGAAAAACACCCTA
TGGTCCCAAGCGGCTCGGGGGGAGATAAAGGGGCGTTTCCCACAATTCCTTTGTGTGAGA
AGACTTTTTTAAGAAAGACGGGAGCGTCCCCCCCCTTATTAAACCCTATTGGAAAAGCCC
CCGTCAC
Restriction Sites NotI-NotI |
ACCN NM_003005
Insert Size 3200 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003005.2, NP_002996.1
RefSeq Size 3199 bp
RefSeq ORF 2493 bp
Locus ID 6403
UniProt ID P16109
Cytogenetics 1q24.2
Domains CCP, CLECT, EGF
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways Cell adhesion molecules (CAMs)
Summary This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_003005" in other vectors (6)
SKU Description Size Price
RC209822 SELP (Myc-DDK-tagged)-Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP) 10 ug
$759.00
RC209822L1 Lenti ORF clone of Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP), Myc-DDK-tagged 10 ug
$1,059.00
RC209822L2 Lenti ORF clone of Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP), mGFP tagged 10 ug
$1,059.00
RC209822L3 Lenti ORF clone of Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP), Myc-DDK-tagged 10 ug
$1,059.00
RC209822L4 Lenti ORF clone of Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP), mGFP tagged 10 ug
$1,059.00
RG209822 SELP (tGFP-tagged) - Human selectin P (granule membrane protein 140kDa, antigen CD62) (SELP) 10 ug
$789.00 $959.00

file-alt Citations

Novel antibodies reactive with sialyl Lewis X in both humans and mice define its critical role in leukocyte trafficking and contact hypersensitivity responses
Matsumura, Hirakawa, Sato et al
J. Biol. Chem. (2015)
AI Summary: OriGene’s hCD62-P/pCMV6-XL5 plasmid was used to generate CHO-K1 cells stably expressing human P-selectin, enabling the authors to analyze P- or E-selectin-dependent leukocyte rolling and assess the functional effects of their novel anti-sLe(x) antibodies.
Snippet: μg/ml MEL-14 (eBioscience) in buffer B containing 0.1% BSA for 10 min at 4 °C. To analyze P- or E-selectin-dependent leukocyte rolling, CHO-K1 cells stably expressing human P-selectin were established by co-transfecting the plasmids hCD62-P/pCMV6-XL5 (OriGENE ... Cite SaveSaving
Showing 1-1 of 1 papers.
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