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COMMD4 (NM_017828) Human Tagged ORF Clone

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SKU
RC209638
COMMD4 (Myc-DDK-tagged)-Human COMM domain containing 4 (COMMD4)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol COMMD4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209638 representing NM_017828
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGTTCCGGTTCTGTGGTGATCTGGACTGTCCCGACTGGGTCCTGGCAGAAATCAGCACGCTGGCCA
AGATGTCCTCTGTGAAGTTGCGGCTGCTCTGCAGCCAGGTACTAAAGGAGCTGCTGGGACAGGGGATTGA
TTATGAGAAGATCCTGAAGCTCACGGCTGACGCCAAGTTTGAGTCAGGCGATGTGAAGGCCACAGTGGCA
GTGCTGAGTTTCATCCTCTCCAGTGCGGCCAAGCACAGTGTCGATGGCGAATCCTTGTCCAGTGAACTGC
AGCAGCTGGGGCTGCCCAAAGAGCACGCGGCCAGCCTGTGCCGCTGTTATGAGGAGAAGCAAAGCCCCTT
GCAGAAGCACTTGCGGGTCTGCAGCCTACGCATGAATAGGTTGGCAGGTGTGGGCTGGCGGGTGGACTAC
ACCCTGAGCTCCAGCCTGCTGCAATCCGTGGAAGAGCCCATGGTGCACCTGCGGCTGGAGGTGGCAGCTG
CCCCAGGGACCCCAGCCCAGCCTGTTGCCATGTCCCTCTCAGCAGACAAGTTCCAGGTCCTCCTGGCAGA
ACTGAAGCAGGCCCAGACCCTGATGAGCTCCCTGGGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209638 representing NM_017828
Red=Cloning site Green=Tags(s)

MRFRFCGDLDCPDWVLAEISTLAKMSSVKLRLLCSQVLKELLGQGIDYEKILKLTADAKFESGDVKATVA
VLSFILSSAAKHSVDGESLSSELQQLGLPKEHAASLCRCYEEKQSPLQKHLRVCSLRMNRLAGVGWRVDY
TLSSSLLQSVEEPMVHLRLEVAAAPGTPAQPVAMSLSADKFQVLLAELKQAQTLMSSLG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017828
ORF Size 597 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017828.5
RefSeq Size 1948 bp
RefSeq ORF 600 bp
Locus ID 54939
UniProt ID Q9H0A8
Cytogenetics 15q24.2
MW 21.6 kDa
Summary May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes (PubMed:21778237). Down-regulates activation of NF-kappa-B.UniProtKB/Swiss-Prot Function
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Documents
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"NM_017828" in other vectors (6)
SKU Description Size Price
RC209638L1 Lenti ORF clone of Human COMM domain containing 4 (COMMD4), Myc-DDK-tagged 10 ug
$600.00
RC209638L2 Lenti ORF clone of Human COMM domain containing 4 (COMMD4), mGFP tagged 10 ug
$600.00
RC209638L3 Lenti ORF clone of Human COMM domain containing 4 (COMMD4), Myc-DDK-tagged 10 ug
$600.00
RC209638L4 Lenti ORF clone of Human COMM domain containing 4 (COMMD4), mGFP tagged 10 ug
$600.00
RG209638 COMMD4 (tGFP-tagged) - Human COMM domain containing 4 (COMMD4) 10 ug
$500.00
SC113915 COMMD4 (untagged)-Human COMM domain containing 4 (COMMD4) 10 ug
$300.00

Plasmid Map for RC209638

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209638
Circular map for RC209638

file-alt Citations

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