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PLA2G4C (NM_003706) Human Tagged ORF Clone

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SKU
RC209567
PLA2G4C (Myc-DDK-tagged)-Human phospholipase A2, group IVC (cytosolic, calcium-independent) (PLA2G4C), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $504.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PLA2G4C
Synonyms CPLA2-gamma
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209567 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAGCTCTGAAGTTTCCATAATTCCTGGGCTCCAGAAAGAAGAAAAGGCGGCCGTGGAGAGACGAA
GACTTCATGTGCTGAAAGCTCTGAAGAAGCTAAGGATTGAGGCTGATGAGGCCCCAGTTGTTGCTGTGCT
GGGCTCAGGCGGAGGACTGCGGGCTCACATTGCCTGCCTTGGGGTCCTGAGTGAGATGAAAGAACAGGGC
CTGTTGGATGCCGTCACGTACCTCGCAGGGGTCTCTGGATCCACTTGGGCAATATCTTCTCTCTACACCA
ATGATGGTGACATGGAAGCTCTCGAGGCTGACCTGAAACATCGATTTACCCGACAGGAGTGGGACTTGGC
TAAGAGCCTACAGAAAACCATCCAAGCAGCGAGGTCTGAGAATTACTCTCTGACCGACTTCTGGGCCTAC
ATGGTTATCTCTAAGCAAACCAGAGAACTGCCGGAGTCTCATTTGTCCAATATGAAGAAGCCCGTGGAAG
AAGGGACACTACCCTACCCGATATTTGCAGCCATTGACAATGACCTGCAACCTTCCTGGCAGGAGGCAAG
AGCACCAGAGACCTGGTTCGAGTTCACCCCTCACCACGCTGGCTTCTCTGCACTGGGGGCCTTTGTTTCC
ATAACCCACTTCGGAAGCAAATTCAAGAAGGGAAGACTGGTCAGAACTCACCCTGAGAGAGACCTGACTT
TCCTGAGAGGTTTATGGGGAAGTGCTCTTGGTAACACTGAAGTCATTAGGGAATACATTTTTGACCAGTT
AAGGAATCTGACCCTGAAAGGTTTATGGAGAAGGGCTGTTGCTAATGCTAAAAGCATTGGACACCTTATT
TTTGCCCGATTACTGAGGCTGCAAGAAAGTTCACAAGGGGAACATCCTCCCCCAGAAGATGAAGGCGGTG
AGCCTGAACACACCTGGCTGACTGAGATGCTCGAGAATTGGACCAGGACCTCCCTGGAAAAGCAGGAGCA
GCCCCATGAGGACCCCGAAAGGAAAGGCTCACTCAGTAACTTGATGGATTTTGTGAAGAAAACAGGCATT
TGCGCTTCAAAGTGGGAATGGGGGACCACTCACAACTTCCTGTACAAACACGGTGGCATCCGGGACAAGA
TAATGAGCAGCCGGAAGCACCTCCACCTGGTGGATGCTGGTTTAGCCATCAACACTCCCTTCCCACTCGT
GCTGCCCCCGACGCGGGAGGTTCACCTCATCCTCTCCTTCGACTTCAGTGCCGGAGATCCTTTCGAGACC
ATCCGGGCTACCACTGACTACTGCCGCCGCCACAAGATCCCCTTTCCCCAAGTAGAAGAGGCTGAGCTGG
ATTTGTGGTCCAAGGCCCCCGCCAGCTGCTACATCCTGAAAGGAGAAACTGGACCAGTGGTGATGCATTT
TCCCCTGTTCAACATAGATGCCTGTGGAGGTGATATTGAGGCATGGAGTGACACATACGACACATTCAAG
CTTGCTGACACCTACACTCTAGATGTGGTGGTGCTACTCTTGGCATTAGCCAAGAAGAATGTCAGGGAAA
ACAAGAAGAAGATCCTTAGAGAGTTGATGAACGTGGCCGGGCTCTACTACCCGAAGGATAGTGCCCGAAG
TTGCTGCTTGGCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209567 protein sequence
Red=Cloning site Green=Tags(s)

MGSSEVSIIPGLQKEEKAAVERRRLHVLKALKKLRIEADEAPVVAVLGSGGGLRAHIACLGVLSEMKEQG
LLDAVTYLAGVSGSTWAISSLYTNDGDMEALEADLKHRFTRQEWDLAKSLQKTIQAARSENYSLTDFWAY
MVISKQTRELPESHLSNMKKPVEEGTLPYPIFAAIDNDLQPSWQEARAPETWFEFTPHHAGFSALGAFVS
ITHFGSKFKKGRLVRTHPERDLTFLRGLWGSALGNTEVIREYIFDQLRNLTLKGLWRRAVANAKSIGHLI
FARLLRLQESSQGEHPPPEDEGGEPEHTWLTEMLENWTRTSLEKQEQPHEDPERKGSLSNLMDFVKKTGI
CASKWEWGTTHNFLYKHGGIRDKIMSSRKHLHLVDAGLAINTPFPLVLPPTREVHLILSFDFSAGDPFET
IRATTDYCRRHKIPFPQVEEAELDLWSKAPASCYILKGETGPVVMHFPLFNIDACGGDIEAWSDTYDTFK
LADTYTLDVVVLLLALAKKNVRENKKKILRELMNVAGLYYPKDSARSCCLA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003706
ORF Size 1623 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003706.3
RefSeq Size 2541 bp
RefSeq ORF 1626 bp
Locus ID 8605
UniProt ID Q9UP65, A0A024QZH0
Cytogenetics 19q13.33
Domains PLA2_B
MW 60.9 kDa
Summary This gene encodes a protein which is a member of the phospholipase A2 enzyme family which hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids, both of which serve as precursors in the production of signaling molecules. The encoded protein has been shown to be a calcium-independent and membrane bound enzyme. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Apr 2009
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Documents
Resources
"NM_003706" in other vectors (4)
SKU Description Size Price
RC209567L3 Lenti ORF clone of Human phospholipase A2, group IVC (cytosolic, calcium-independent) (PLA2G4C), transcript variant 1, Myc-DDK-tagged 10 ug
$804.00
RC209567L4 Lenti ORF clone of Human phospholipase A2, group IVC (cytosolic, calcium-independent) (PLA2G4C), transcript variant 1, mGFP tagged 10 ug
$804.00
RG209567 PLA2G4C (tGFP-tagged) - Human phospholipase A2, group IVC (cytosolic, calcium-independent) (PLA2G4C), transcript variant 1 10 ug
$489.00 $704.00
SC117817 PLA2G4C (untagged)-Human phospholipase A2, group IVC (cytosolic, calcium-independent) (PLA2G4C), transcript variant 1 10 ug
$505.00

Plasmid Map for RC209567

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209567
Circular map for RC209567

file-alt Citations

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