RIP3 (RIPK3) (NM_006871) Human Tagged ORF Clone
- TrueORF®
SKU
RC209549
RIPK3 (Myc-DDK-tagged)-Human receptor-interacting serine-threonine kinase 3 (RIPK3)
$723.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | RIP3 |
| Synonyms | RIP3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_006871 |
| ORF Size | 1554 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006871.4 |
| RefSeq Size | 1940 bp |
| RefSeq ORF | 1557 bp |
| Locus ID | 11035 |
| UniProt ID | Q9Y572 |
| Cytogenetics | 14q12 |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Cytosolic DNA-sensing pathway |
| MW | 56.9 kDa |
| Summary | The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006871" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC209549L1 | Lenti ORF clone of Human receptor-interacting serine-threonine kinase 3 (RIPK3), Myc-DDK-tagged | 10 ug |
$1,023.00
|
|
| RC209549L2 | Lenti ORF clone of Human receptor-interacting serine-threonine kinase 3 (RIPK3), mGFP tagged | 10 ug |
$1,023.00
|
|
| RC209549L3 | Lenti ORF clone of Human receptor-interacting serine-threonine kinase 3 (RIPK3), Myc-DDK-tagged | 10 ug |
$1,023.00
|
|
| RC209549L4 | Lenti ORF clone of Human receptor-interacting serine-threonine kinase 3 (RIPK3), mGFP tagged | 10 ug |
$1,023.00
|
|
| RG209549 | RIPK3 (tGFP-tagged) - Human receptor-interacting serine-threonine kinase 3 (RIPK3) | 10 ug |
$923.00
|
|
| SC303834 | RIPK3 (untagged)-Human receptor-interacting serine-threonine kinase 3 (RIPK3) | 10 ug |
$725.00
|
|
Citations
Mismatched effects of receptor interacting protein kinase-3 on hepatic steatosis and inflammation in non-alcoholic fatty liver disease
Saeed, Jun, Jang et al
World J. Gastroenterol. (2018) 24 (48), 5477-5490 DOI: 10.3748/wjg.v24.i48.5477
Saeed, Jun, Jang et al
World J. Gastroenterol. (2018) 24 (48), 5477-5490 DOI: 10.3748/wjg.v24.i48.5477
AI Summary: OriGene's TrueORF cDNA Clones were used to amplify human RIP3 coding regions, enabling the construction of RIP3 non-viral expression vectors for functional studies in this paper.
Snippet: Germany). RIP3 non-viral vector construction. The human RIP3 (NM_006871) coding regions were amplified by polymerase chain reaction (PCR) using TrueORF cDNA Clones (Origene, Rockville, MD, United States) for genes
ATP-Competitive MLKL Binders Have No Functional Impact on Necroptosis
Ma, Marcotte, Paramasivam et al
PLoS ONE (2016) 11 (11), e0165983 DOI: 10.1371/journal.pone.0165983
Ma, Marcotte, Paramasivam et al
PLoS ONE (2016) 11 (11), e0165983 DOI: 10.1371/journal.pone.0165983
Showing 1-2 of 2 papers.
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