KHDC4 (NM_014949) Human Tagged ORF Clone

SKU
RC209536
KIAA0907 (Myc-DDK-tagged)-Human KIAA0907 (KIAA0907)
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KHDC4
Synonyms BLOM7; KIAA0907; SNORA80EHG
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209536 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGCGGGGAGCGCGACACATCCTGGAGCTGGCGGGCGCCGCAGCAAATGGGACCAACCAGCTCCAG
CCCCACTTCTCTTCCTCCCGCCAGCGGCCCCAGGTGGGGAGGTCACCAGCAGTGGGGGAAGTCCTGGGGG
CACCACAGCTGCTCCTTCAGGAGCCTTGGATGCTGCTGCTGCTGTGGCTGCCAAGATTAATGCCATGCTC
ATGGCAAAAGGGAAGCTGAAACCAACTCAGAATGCTTCTGAGAAGCTTCAGGCTCCTGGCAAAGGCCTAA
CTAGCAATAAAAGCAAGGATGACCTGGTGGTAGCTGAAGTAGAAATTAATGATGTGCCTCTCACATGTAG
GAACTTGCTGACTCGAGGACAGACTCAAGACGAGATCAGCCGACTTAGTGGGGCTGCAGTATCAACTCGA
GGGAGGTTCATGACAACTGAGGAAAAAGCCAAAGTGGGACCAGGGGATCGTCCATTATATCTTCATGTTC
AGGGCCAGACACGGGAATTAGTGGACAGAGCTGTAAACCGGATCAAAGAAATTATCACCAATGGAGTGGT
AAAAGCTGCCACAGGAACAAGTCCAACTTTTAATGGTGCAACAGTAACTGTCTATCACCAGCCAGCACCC
ATCGCTCAGTTGTCTCCAGCTGTTAGCCAGAAGCCTCCCTTCCAGTCAGGGATGCATTATGTTCAAGATA
AATTATTTGTGGGTCTAGAACATGCTGTACCCACTTTTAATGTCAAGGAGAAGGTGGAAGGTCCAGGCTG
CTCCTATTTGCAGCACATTCAGATTGAAACAGGTGCCAAAGTCTTCCTGCGGGGCAAAGGTTCAGGCTGC
ATTGAGCCAGCATCTGGCCGAGAAGCTTTTGAACCTATGTATATTTACATCAGTCACCCCAAACCAGAAG
GCCTGGCTGCTGCCAAGAAGCTTTGTGAGAATCTTTTGCAAACAGTTCATGCTGAATACTCTAGATTTGT
GAATCAGATTAATACTGCTGTACCTTTACCAGGCTATACACAACCCTCTGCTATAAGTAGTGTCCCTCCT
CAACCACCATATTATCCATCCAATGGCTATCAGTCTGGTTACCCTGTTGTTCCCCCTCCTCAGCAGCCAG
TTCAACCTCCCTACGGAGTACCAAGCATAGTGCCACCAGCTGTTTCATTAGCACCTGGAGTCTTGCCGGC
ATTACCTACTGGAGTCCCACCTGTGCCAACACAATACCCGATAACACAAGTGCAGCCTCCAGCTAGCACT
GGACAGAGTCCGATGGGTGGTCCTTTTATTCCTGCTGCTCCTGTCAAAACTGCCTTGCCTGCTGGCCCCC
AGCCCCAGCCCCAGCCCCAGCCCCCACTCCCAAGTCAGCCCCAGGCACAGAAGAGACGATTCACAGAGGA
GCTACCAGATGAACGGGAATCTGGACTGCTTGGATACCAGCATGGACCCATTCATATGACTAATTTAGGT
ACAGGCTTCTCCAGTCAGAATGAGATTGAAGGTGCAGGATCGAAGCCAGCAAGTTCCTCAGGCAAAGAGA
GAGAGAGGGACAGGCAGTTGATGCCTCCACCAGCCTTTCCAGTGACTGGAATAAAAACAGAGTCCGATGA
AAGGAATGGGTCTGGGACCTTAACAGGGAGCCATGATTATCCAGCCAAGAAGATGAAAACTACAGAGAAG
GGATTTGGCTTGGTGGCTTATGCTGCAGATTCATCTGATGAAGAGGAGGAACATGGAGGTCATAAAAATG
CAAGTAGTTTTCCACAGGGCTGGAGTTTGGGATACCAATATCCTTCATCACAACCACGAGCTAAACAACA
GATGCCATTCTGGATGGCTCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC209536 protein sequence
Red=Cloning site Green=Tags(s)

MSAGSATHPGAGGRRSKWDQPAPAPLLFLPPAAPGGEVTSSGGSPGGTTAAPSGALDAAAAVAAKINAML
MAKGKLKPTQNASEKLQAPGKGLTSNKSKDDLVVAEVEINDVPLTCRNLLTRGQTQDEISRLSGAAVSTR
GRFMTTEEKAKVGPGDRPLYLHVQGQTRELVDRAVNRIKEIITNGVVKAATGTSPTFNGATVTVYHQPAP
IAQLSPAVSQKPPFQSGMHYVQDKLFVGLEHAVPTFNVKEKVEGPGCSYLQHIQIETGAKVFLRGKGSGC
IEPASGREAFEPMYIYISHPKPEGLAAAKKLCENLLQTVHAEYSRFVNQINTAVPLPGYTQPSAISSVPP
QPPYYPSNGYQSGYPVVPPPQQPVQPPYGVPSIVPPAVSLAPGVLPALPTGVPPVPTQYPITQVQPPAST
GQSPMGGPFIPAAPVKTALPAGPQPQPQPQPPLPSQPQAQKRRFTEELPDERESGLLGYQHGPIHMTNLG
TGFSSQNEIEGAGSKPASSSGKERERDRQLMPPPAFPVTGIKTESDERNGSGTLTGSHDYPAKKMKTTEK
GFGLVAYAADSSDEEEEHGGHKNASSFPQGWSLGYQYPSSQPRAKQQMPFWMAP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_014949
ORF Size 1842 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014949.4
RefSeq Size 2960 bp
RefSeq ORF 1845 bp
Locus ID 22889
UniProt ID Q7Z7F0
Cytogenetics 1q22
MW 64.8 kDa
Summary RNA-binding protein involved in pre-mRNA splicing (PubMed:19641227). Interacts with the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome (PubMed:19641227). Involved in regulating splice site selection (PubMed:19641227). Binds preferentially RNA with A/C rich sequences and poly-C stretches (PubMed:23144703).[UniProtKB/Swiss-Prot Function]
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Citations

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