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MCCC2 (NM_022132) Human Tagged ORF Clone

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SKU
RC209488
MCCC2 (Myc-DDK-tagged)-Human methylcrotonoyl-CoA carboxylase 2 (beta) (MCCC2), nuclear gene encoding mitochondrial protein
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $524.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MCCC2
Synonyms MCCB; MCCCbeta
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209488 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGGCCGTCCTGAGGTTAGCCCTGCGGCCGTGTGCCCGCGCCTCTCCCGCCGGGCCGCGCGCCTATC
ACGGGGACTCGGTGGCCTCGCTGGGCACCCAGCCGGACTTGGGCTCTGCCCTCTACCAGGAGAACTACAA
GCAGATGAAAGCACTAGTAAATCAGCTCCATGAACGAGTGGAGCATATAAAACTAGGAGGTGGTGAGAAA
GCCCGAGCACTTCACATATCAAGAGGAAAACTATTGCCCAGAGAAAGAATTGACAATCTCATAGACCCAG
GGTCTCCATTTCTGGAATTATCCCAGTTTGCAGGTTACCAGTTATATGACAATGAGGAGGTGCCAGGAGG
TGGCATTATTACAGGCATTGGAAGAGTATCAGGAGTAGAATGCATGATTATTGCCAATGATGCCACCGTC
AAAGGAGGTGCCTACTACCCAGTGACTGTGAAAAAACAATTACGGGCCCAAGAAATTGCCATGCAAAACA
GGCTCCCCTGCATCTACTTAGTTGATTCGGGAGGAGCATACTTACCTCGACAAGCAGATGTGTTTCCAGA
TCGAGACCACTTTGGCCGTACATTCTATAATCAGGCAATTATGTCTTCTAAAAATATTGCACAGATCGCA
GTGGTCATGGGCTCCTGCACCGCAGGAGGAGCCTATGTGCCTGCCATGGCTGATGAAAACATCATTGTAC
GCAAGCAGGGTACCATTTTCTTGGCAGGACCCCCCTTGGTTAAAGCGGCAACTGGGGAAGAAGTATCTGC
TGAGGATCTTGGAGGTGCTGATCTTCATTGCAGAAAGTCTGGAGTAAGTGACCACTGGGCTTTGGATGAT
CATCATGCCCTTCACTTAACTAGGAAGGTTGTGAGGAATCTAAATTATCAGAAGAAATTGGATGTCACCA
TTGAACCTTCTGAAGAGCCTTTATTTCCTGCTGATGAATTGTATGGAATAGTTGGTGCTAACCTTAAGAG
GAGCTTTGATGTCCGAGAGGTCATTGCTAGAATCGTGGATGGAAGCAGATTCACTGAGTTCAAAGCCTTT
TATGGAGACACATTAGTTACAGGATTTGCTCGAATATTTGGGTACCCAGTAGGTATCGTTGGAAACAACG
GAGTTCTCTTTTCTGAATCTGCAAAAAAGGGTACTCACTTTGTCCAGTTATGCTGCCAAAGAAATATTCC
TCTGCTGTTCCTTCAAAACATTACTGGATTTATGGTTGGTAGAGAGTATGAAGCTGAAGGAATTGCCAAG
GATGGTGCCAAGATGGTGGCCGCTGTGGCCTGTGCCCAAGTGCCTAAGATAACCCTCATCATTGGGGGCT
CCTATGGAGCCGGAAACTATGGGATGTGTGGCAGAGCGTATAGCCCAAGATTTCTCTACATTTGGCCAAA
TGCTCGTATCTCAGTGATGGGAGGAGAGCAGGCAGCCAATGTGTTGGCCACGATAACAAAGGACCAAAGA
GCCCGGGAAGGAAAGCAGTTCTCCAGTGCTGATGAAGCGGCTTTAAAAGAGCCCATCATTAAGAAGTTTG
AAGAGGAAGGAAACCCTTACTATTCCAGCGCAAGGGTATGGGATGATGGGATCATTGATCCAGCAGACAC
CAGACTGGTCTTGGGTCTCAGTTTTAGTGCAGCCCTCAACGCACCAATAGAGAAGACTGACTTCGGTATC
TTCAGGATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209488 protein sequence
Red=Cloning site Green=Tags(s)

MWAVLRLALRPCARASPAGPRAYHGDSVASLGTQPDLGSALYQENYKQMKALVNQLHERVEHIKLGGGEK
ARALHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITGIGRVSGVECMIIANDATV
KGGAYYPVTVKKQLRAQEIAMQNRLPCIYLVDSGGAYLPRQADVFPDRDHFGRTFYNQAIMSSKNIAQIA
VVMGSCTAGGAYVPAMADENIIVRKQGTIFLAGPPLVKAATGEEVSAEDLGGADLHCRKSGVSDHWALDD
HHALHLTRKVVRNLNYQKKLDVTIEPSEEPLFPADELYGIVGANLKRSFDVREVIARIVDGSRFTEFKAF
YGDTLVTGFARIFGYPVGIVGNNGVLFSESAKKGTHFVQLCCQRNIPLLFLQNITGFMVGREYEAEGIAK
DGAKMVAAVACAQVPKITLIIGGSYGAGNYGMCGRAYSPRFLYIWPNARISVMGGEQAANVLATITKDQR
AREGKQFSSADEAALKEPIIKKFEEEGNPYYSSARVWDDGIIDPADTRLVLGLSFSAALNAPIEKTDFGI
FRM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022132
ORF Size 1689 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022132.3, NP_071415.1
RefSeq Size 3696 bp
RefSeq ORF 1692 bp
Locus ID 64087
UniProt ID Q9HCC0, A0A140VK29
Cytogenetics 5q13.2
Domains Carboxyl_trans
Protein Families Druggable Genome
Protein Pathways leucine and isoleucine degradation, Metabolic pathways, Valine
MW 61.3 kDa
Summary This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, May 2018
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"NM_022132" in other vectors (5)
SKU Description Size Price
RC209488L2 Lenti ORF clone of Human methylcrotonoyl-CoA carboxylase 2 (beta) (MCCC2), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$824.00
RC209488L3 Lenti ORF clone of Human methylcrotonoyl-CoA carboxylase 2 (beta) (MCCC2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$824.00
RC209488L4 Lenti ORF clone of Human methylcrotonoyl-CoA carboxylase 2 (beta) (MCCC2), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$824.00
RG209488 MCCC2 (tGFP-tagged) - Human methylcrotonoyl-CoA carboxylase 2 (beta) (MCCC2), nuclear gene encoding mitochondrial protein 10 ug
$489.00 $724.00
SC125472 MCCC2 (untagged)-Human methylcrotonoyl-CoA carboxylase 2 (beta) (MCCC2), nuclear gene encoding mitochondrial protein 10 ug
$525.00

Plasmid Map for RC209488

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209488
Circular map for RC209488

file-alt Citations

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