ATPBD4 (DPH6) (NM_080650) Human Tagged ORF Clone

  • Product Brand Image
SKU
RC209471
ATPBD4 (Myc-DDK-tagged)-Human ATP binding domain 4 (ATPBD4), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ATPBD4
Synonyms ATPBD4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209471 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGTCGCGGCTCTGATCAGTGGTGGGAAGGACAGCTGCTATAATATGATGCAGTGCATTGCTGCTG
GGCATCAGATCGTTGCTTTAGCAAATCTAAGACCAGCTGAAAACCAAGTGGGGTCTGATGAACTGGATAG
CTACATGTATCAGACAGTGGGGCACCATGCCATTGACTTGTATGCAGAAGCAATGGCTCTTCCCCTCTAT
CGCCGAACCATAAGAGGAAGGAGCTTGGATACAAGACAAGTGTACACCAAATGTGAAGGTGATGAGGTTG
AAGATCTCTATGAGCTTTTGAAACTTGTTAAGGAAAAAGAAGAAGTAGAGGGGATATCAGTAGGTGCTAT
ACTTTCTGACTATCAGCGTATTCGAGTGGAAAATGTGTGTAAAAGGCTTAATCTCCAGCCTTTAGCTTAT
CTTTGGCAGAGAAACCAGGAAGATTTGCTCAGAGAGATGATATCATCTAACATTCAAGCAATGATCATCA
AAGTAGCAGCTTTGGGTTTAGATCCTGATAAGCATCTTGGGAAAACCCTGGATCAAATGGAGCCTTATCT
CATAGAGCTTTCTAAGAAGTATGGAGTACATGTTTGTGGAGAAGGTGGAGAGTATGAAACTTTCACTTTG
GATTGCCCTCTATTTAAGAAGAAAATAATTGTGGATTCATCAGAAGTAGTCATACATTCAGCTGATGCAT
TTGCACCTGTGGCTTATCTACGCTTTTTAGAATTGCACTTGGAGGACAAGGTGTCCTCAGTGCCTGACAA
CTACAGAACATCTAATTATATATATAATTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC209471 protein sequence
Red=Cloning site Green=Tags(s)

MRVAALISGGKDSCYNMMQCIAAGHQIVALANLRPAENQVGSDELDSYMYQTVGHHAIDLYAEAMALPLY
RRTIRGRSLDTRQVYTKCEGDEVEDLYELLKLVKEKEEVEGISVGAILSDYQRIRVENVCKRLNLQPLAY
LWQRNQEDLLREMISSNIQAMIIKVAALGLDPDKHLGKTLDQMEPYLIELSKKYGVHVCGEGGEYETFTL
DCPLFKKKIIVDSSEVVIHSADAFAPVAYLRFLELHLEDKVSSVPDNYRTSNYIYNF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080650
ORF Size 801 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080650.4
RefSeq Size 2139 bp
RefSeq ORF 804 bp
Locus ID 89978
UniProt ID Q7L8W6
Cytogenetics 15q14
MW 30.3 kDa
Summary Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor (EEF2) to diphthamide (By similarity).UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_080650" in other vectors (5)
SKU Description Size Price
RC209471L3 Lenti ORF clone of Human ATP binding domain 4 (ATPBD4), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC209471L4 Lenti ORF clone of Human ATP binding domain 4 (ATPBD4), transcript variant 1, mGFP tagged 10 ug
$600.00
RG209471 ATPBD4 (tGFP-tagged) - Human ATP binding domain 4 (ATPBD4), transcript variant 1 10 ug
$489.00 $500.00
SC120394 ATPBD4 (untagged)-Human ATP binding domain 4 (ATPBD4), transcript variant 1 10 ug
$300.00
SC322707 ATPBD4 (untagged)-Human ATP binding domain 4 (ATPBD4), transcript variant 1 10 ug
$300.00

Plasmid Map for RC209471

Download sequence for

Cloning scheme for RC209471

Circular map for RC209471

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products