RG9MTD3 (TRMT10B) (NM_144964) Human Tagged ORF Clone

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SKU
RC209185
TRMT10B (Myc-DDK-tagged)-Human RNA (guanine-9-) methyltransferase domain containing 3 (RG9MTD3)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RG9MTD3
Synonyms bA3J10.9; RG9MTD3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209185 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTGGAAATTGGAAGGGAGTACTCAGAAAGTAGAGTCACCTGTGCTGCAGGGGCAAGAAGGCATCC
TAGAGGAGACAGGTGAAGATGGACTTCCTGAAGGCTTCCAGCTTCTGCAGATCGATGCGGAAGGCGAGTG
CCAGGAGGGAGAGATCCTGGCCACAGGCAGTACGGCATGGTGCTCGAAAAATGTCCAGAGAAAACAGAGA
CACTGGGAAAAGATAGTTGCAGCAAAGAAGAGCAAAAGAAAGCAAGAAAAAGAACGAAGAAAAGCCAATC
GTGCAGAAAATCCAGGCATTTGCCCCCAGCACAGCAAACGTTTCCTGAGAGCTCTAACCAAAGACAAACT
TTTGGAAGCCAAACACTCAGGACCAAGACTATGTATCGATTTGAGTATGACCCACTACATGTCAAAGAAG
GAATTAAGTAGACTGGCTGGACAGATTCGAAGGTTGTATGGTTCAAACAAAAAAGCTGACAGGCCATTTT
GGATCTGCCTCACTGGATTCACAACAGACAGTCCCCTTTATGAAGAGTGTGTGAGGATGAATGATGGATT
TTCTAGTTACCTGTTAGACATAACAGAAGAAGACTGCTTTAGTTTATTTCCCTTGGAAACCCTTGTGTAC
CTGACTCCTGACTCAGAACACGCTCTTGAAGATGTTGATCTAAACAAAGTTTACATCCTCGGTGGGCTTG
TGGATGAAAGCATTCAGAAGAAGGTGACATTTCAAAAGGCCCGGGAATACTCTGTCAAGACCGCACGCTT
GCCAATCCAGGAATACATGGTCAGAAACCAGAATGGGAAAAACTATCATTCAGAGATACTGGCCATCAAT
CAAGTGTTTGATATCCTGTCCACTTACTTAGAGACTCACAACTGGCCTGAAGCATTGAAGAAAGGAGTTT
CTTCAGGAAAAGGCTATATTCTTCGGAACTCAGTGGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC209185 protein sequence
Red=Cloning site Green=Tags(s)

MDWKLEGSTQKVESPVLQGQEGILEETGEDGLPEGFQLLQIDAEGECQEGEILATGSTAWCSKNVQRKQR
HWEKIVAAKKSKRKQEKERRKANRAENPGICPQHSKRFLRALTKDKLLEAKHSGPRLCIDLSMTHYMSKK
ELSRLAGQIRRLYGSNKKADRPFWICLTGFTTDSPLYEECVRMNDGFSSYLLDITEEDCFSLFPLETLVY
LTPDSEHALEDVDLNKVYILGGLVDESIQKKVTFQKAREYSVKTARLPIQEYMVRNQNGKNYHSEILAIN
QVFDILSTYLETHNWPEALKKGVSSGKGYILRNSVE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144964
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144964.4
RefSeq Size 2310 bp
RefSeq ORF 951 bp
Locus ID 158234
UniProt ID Q6PF06
Cytogenetics 9p13.2
MW 36.1 kDa
Summary S-adenosyl-L-methionine-dependent guanine N(1)-methyltransferase that catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in tRNAs (PubMed:23042678). Probably not able to catalyze formation of N(1)-methyladenine at position 9 (m1A9) in tRNAs (PubMed:23042678).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_144964" in other vectors (4)
SKU Description Size Price
RC209185L3 Lenti ORF clone of Human RNA (guanine-9-) methyltransferase domain containing 3 (RG9MTD3), Myc-DDK-tagged 10 ug
$600.00
RC209185L4 Lenti ORF clone of Human RNA (guanine-9-) methyltransferase domain containing 3 (RG9MTD3), mGFP tagged 10 ug
$600.00
RG209185 TRMT10B (tGFP-tagged) - Human RNA (guanine-9-) methyltransferase domain containing 3 (RG9MTD3) 10 ug
$489.00 $500.00
SC125675 TRMT10B (untagged)-Human RNA (guanine-9-) methyltransferase domain containing 3 (RG9MTD3) 10 ug
$300.00

Plasmid Map for RC209185

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Cloning scheme for RC209185

Circular map for RC209185

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