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RG9MTD3 (TRMT10B) (NM_144964) Human Untagged Clone

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SKU
SC125675
TRMT10B (untagged)-Human RNA (guanine-9-) methyltransferase domain containing 3 (RG9MTD3)
  $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RG9MTD3
Synonyms bA3J10.9; RG9MTD3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_144964 edited
GCGCGCCGCTGCCGCTGCGTGGGGGTGAGGGGATCAGTCTGGTGGAAAGGACAGAGGACT
AAGTCCATGGACTGGAAATTGGAAGGGAGTACTCAGAAAGTAGAGTCACCTGTGCTGCAG
GGGCAAGAAGGCATCCTAGAGGAGACAGGTGAAGATGGACTTCCTGAAGGCTTCCAGCTT
CTGCAGATCGATGCGGAAGGCGAGTGCCAGGAGGGAGAGATCCTGGCCACAGGCAGTACG
GCATGGTGCTCGAAAAATGTCCAGAGAAAACAGAGACACTGGGAAAAGATAGTTGCAGCA
AAGAAGAGCAAAAGAAAGCAAGAAAAAGAACGAAGAAAAGCCAATCGTGCAGAAAATCCA
GGCATTTGCCCCCAGCACAGCAAACGTTTCCTGAGAGCTCTAACCAAAGACAAACTTTTG
GAAGCCAAACACTCAGGACCAAGACTATGTATCGATTTGAGTATGACCCACTACATGTCA
AAGAAGGAATTAAGTAGACTGGCTGGACAGATTCGAAGGTTGTATGGTTCAAACAAAAAA
GCTGACAGGCCATTTTGGATCTGCCTCACTGGATTCACAACAGACAGTCCCCTTTATGAA
GAGTGTGTGAGGATGAATGATGGATTTTCTAGTTACCTGTTAGACATAACAGAAGAAGAC
TGCTTTAGTTTATTTCCCTTGGAAACCCTTGTGTACCTGACTCCTGACTCAGAACACGCT
CTTGAAGATGTTGATCTAAACAAAGTTTACATCCTCGGTGGGCTTGTGGATGAAAGCATT
CAGAAGAAGGTGACATTTCAAAAGGCCCGGGAATACTCTGTCAAGACCGCACGCTTGCCA
ATCCAGGAATACATGGTCAGAAACCAGAATGGGAAAAACTATCATTCAGAGATACTGGCC
ATCAATCAAGTGTTTGATATCCTGTCCACTTACTTAGAGACTCACAACTGGCCTGAAGCA
TTGAAGAAAGGAGTTTCTTCAGGAAAAGGCTATATTCTTCGGAACTCAGTGGAATGATGG
GCCTAAGATTGCAGCTGCGTGGCCAGGTGCTCACGCCGTAATGCCAACACTTTGGTAGAC
CGAAGTGGGCAGATCACCTGAGGTCAGGAGTTCACGACCAGCCTGGCCAACATGGTGAAA
CCCTTCTCTACTGAAAATACAAAAATTAGCCAGGTGTGGTGGCGCATACCTGTAGTCCCA
GCTACTTGGGAGGCTGAGGCAGGAGAATCACTTGAACTCGGGAGGCGAAGGTTGCAGTGA
GCCGAGATTTCACCAGTGCACTCCAGCCTGGGTGACAGAGCAAGACTCCATCTCAAAAAA
AAAATAAATAAAAAAAAATGCAGCTGCAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAA
AAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_144964 unedited
NGGGTCANATATTGTATACGACTCATATAGGCGGCCGCATAACTTCGTATAGCATACATT
ATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGCTCTAGCGAGG
TGACAGCGTAGAACCAGGCGCGCCGCTGCCGCTGCGTGGGGGTGAGGGGATCAGTCTGGT
GGAAAGGACAGAGGACTAAGTCCATGGACTGGAAATTGGAAGGGAGTACTCAGAAAGTAG
AGTCACCTGTGCTGCAGGGGCAAGAAGGCATCCTAGAGGAGACAGGTGAAGATGGACTTC
CTGAAGGCTTCCAGCTTCTGCAGATCGATGCGGAAGGCGAGTGCCAGGAGGGAGAGATCC
TGGCCACAGGCAGTACGGCATGGTGCTCGAAAAATGTCCAGAGAAAACAGAGACACTGGG
AAAAGATAGTTGCAGCAAAGAAGAGCAAAAGAAAGCAAGAAAAAGAACGAAGAAAAGCCA
ATCGTGCAGAAAATCCAGGCATTTGCCCCCAGCACAGCAAACGTTTCCTGAGAGCTCTAA
CCAAAGACAAACTTTTGGAAGCCAAACACTCAGGACCAAGACTATGTATCGATTTGAGTA
TGACCCACTACATGTCAAAGAAGGAATTAAGTAGACTGGCTGGACAGATTCGAAGGTTGT
ATGGTTCANACAAAAAAGCTGACAGGCCATTTTGGATCTGCCTCACTGGATTCACAACAG
ACAGTCCCCTTTATGAAGAGTGTGTGAAGATGAATGATGGATTTTCTAGTTACCTGTTAN
GACATACAGAAGAAGACTGCTTTAGTTTATTTCCCCTTGGAACCCTTGTGTACCTGACTC
CTGACTCAGACACGCTCTTGAAGATGTTTGATCTAAACAAGTTACATCCCTCGTGGGCTT
GTGGATGAAAGCT
Restriction Sites NotI-NotI |
ACCN NM_144964
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144964.1, NP_659401.1
RefSeq Size 1828 bp
RefSeq ORF 960 bp
Locus ID 158234
UniProt ID Q6PF06
Cytogenetics 9p13.2
Summary S-adenosyl-L-methionine-dependent guanine N(1)-methyltransferase that catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in tRNAs (PubMed:23042678). Probably not able to catalyze formation of N(1)-methyladenine at position 9 (m1A9) in tRNAs (PubMed:23042678).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longest isoform (a).
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Documents
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"NM_144964" in other vectors (4)
SKU Description Size Price
RC209185 TRMT10B (Myc-DDK-tagged)-Human RNA (guanine-9-) methyltransferase domain containing 3 (RG9MTD3) 10 ug
$300.00
RC209185L3 Lenti ORF clone of Human RNA (guanine-9-) methyltransferase domain containing 3 (RG9MTD3), Myc-DDK-tagged 10 ug
$600.00
RC209185L4 Lenti ORF clone of Human RNA (guanine-9-) methyltransferase domain containing 3 (RG9MTD3), mGFP tagged 10 ug
$600.00
RG209185 TRMT10B (tGFP-tagged) - Human RNA (guanine-9-) methyltransferase domain containing 3 (RG9MTD3) 10 ug
$489.00 $500.00

file-alt Citations

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