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CC2D1A (NM_017721) Human Tagged ORF Clone

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SKU
RC209105
CC2D1A (Myc-DDK-tagged)-Human coiled-coil and C2 domain containing 1A (CC2D1A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $871.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CC2D1A
Synonyms Aki-1; FREUD-1; Freud-1/Aki1; Lgd2; MRT3; TAPE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209105 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACAAGAGGAAAGGACCCCCGGGACCCCCGGGCAGAGGCGCCGCGGCCGCCCGCCAGCTGGGCCTGC
TGGTTGACCTCTCCCCAGATGGCCTGATGATCCCTGAGGACGGGGCTAACGATGAAGAACTGGAGGCTGA
GTTCTTGGCTTTGGTCGGGGGCCAGCCCCCAGCCCTGGAGAAGCTCAAAGGCAAAGGTCCCTTGCCGATG
GAGGCCATTGAGAAGATGGCCAGCCTGTGCATGAGAGACCCGGATGAGGATGAGGAGGAGGGGACGGATG
AGGACGACTTGGAGGCTGATGATGACCTGCTGGCGGAGCTAAATGAGGTCCTTGGAGAGGAGCAGAAGGC
TTCAGAGACCCCACCTCCTGTGGCCCAGCCGAAGCCTGAGGCCCCTCATCCGGGGCTGGAGACCACCTTG
CAGGAGAGGCTGGCGCTCTATCAGACAGCAATTGAAAGCGCCAGACAAGCTGGAGACAGCGCCAAGATGC
GGCGCTACGATCGGGGGCTTAAAACACTGGAAAACCTGCTCGCCTCCATCCGTAAGGGCAATGCCATTGA
CGAAGCGGACATCCCGCCGCCAGTGGCCATAGGAAAAGGCCCGGCGTCCACGCCTACCTACAGCCCTGCA
CCCACCCAGCCGGCCCCTAGAATCGCGTCAGCCCCAGAGCCCAGGGTCACCCTGGAGGGACCTTCTGCCA
CCGCCCCAGCCTCATCTCCAGGCTTGGCTAAGCCCCAGATGCCCCCAGGTCCCTGCAGCCCTGGCCCTCT
GGCCCAGTTGCAGAGCCGCCAGCGCGACTACAAGCTGGCTGCCCTCCACGCCAAGCAGCAGGGAGATACC
ACTGCTGCCGCTAGACACTTCCGCGTGGCTAAGAGCTTTGATGCTGTCTTGGAGGCCCTGAGCCGGGGTG
AGCCCGTGGACCTCTCCTGCCTGCCCCCTCCACCCGACCAGCTGCCCCCAGACCCACCGTCACCACCGTC
GCAGCCTCCGACCCCCGCTACGGCGCCCTCCACAACAGAGGTGCCCCCACCCCCGAGGACCCTGCTGGAG
GCGCTGGAGCAGCGGATGGAGCGGTACCAGGTGGCCGCAGCCCAGGCCAAGAGCAAGGGGGACCAGCGGA
AAGCTCGAATGCACGAGCGCATCGTCAAGCAATACCAAGATGCCATCCGAGCCCACAAGGCTGGCCGAGC
CGTGGATGTCGCTGAATTGCCCGTGCCCCCAGGCTTCCCCCCAATCCAGGGCCTGGAGGCCACCAAGCCC
ACCCAGCAGAGTCTGGTGGGTGTCCTGGAGACTGCCATGAAGCTGGCCAACCAGGATGAAGGCCCAGAGG
ATGAAGAGGATGAGGTGCCTAAGAAGCAGAACAGCCCTGTGGCCCCCACAGCCCAGCCCAAAGCCCCACC
CTCAAGAACTCCCCAGTCGGGATCAGCCCCAACAGCCAAAGCGCCCCCCAAAGCCACATCCACCAGAGCC
CAGCAGCAGCTGGCCTTCCTAGAGGGCCGCAAGAAGCAGCTCCTGCAGGCCGCACTGCGAGCCAAGCAGA
AAAACGACGTGGAGGGTGCCAAGATGCACCTGCGCCAAGCCAAGGGACTGGAGCCTATGCTGGAGGCCTC
GCGCAATGGGCTGCCTGTGGACATCACCAAGGTGCCGCCTGCCCCTGTCAACAAGGACGACTTTGCCCTG
GTCCAGCGGCCTGGCCCGGGTCTGTCTCAGGAGGCCGCCCGGCGCTATGGTGAACTCACCAAGCTCATAC
GGCAGCAGCACGAGATGTGCCTGAACCACTCAAACCAATTCACCCAGCTGGGCAACATCACTGAAACCAC
CAAGTTTGAAAAGTTGGCGGAGGACTGTAAGCGGAGCATGGACATTCTGAAGCAAGCCTTCGTCCGGGGT
CTCCCCACGCCCACCGCCCGCTTTGAGCAAAGGACCTTCAGCGTCATCAAGATCTTCCCTGACCTCAGCA
GCAACGACATGCTCCTCTTCATCGTGAAGGGCATCAACTTGCCCACACCCCCAGGACTGTCCCCTGGCGA
TCTGGATGTCTTTGTTCGGTTTGACTTCCCCTATCCCAACGTGGAAGAAGCTCAGAAAGACAAGACCAGT
GTGATCAAGAACACAGACTCCCCTGAGTTCAAGGAGCAGTTCAAACTCTGCATCAACCGCAGCCACCGTG
GCTTCCGAAGGGCCATCCAGACCAAGGGCATCAAGTTCGAAGTGGTTCACAAGGGGGGGCTGTTCAAGAC
TGACCGGGTGCTGGGGACAGCCCAGCTGAAGCTGGATGCACTGGAGATAGCATGTGAGGTCCGGGAGATC
CTTGAGGTCCTGGATGGTCGCCGGCCCACAGGGGGGCGACTGGAGGTAATGGTCCGGATTCGGGAGCCAC
TGACAGCCCAGCAGTTGGAGACGACGACAGAGAGGTGGCTGGTCATTGACCCTGTGCCGGCAGCTGTGCC
CACACAGGTTGCTGGGCCCAAAGGGAAGGCCCCTCCTGTGCCTGCCCCTGCAAGGGAGTCAGGGAACAGA
TCAGCCCGGCCCCTGCATAGCCTCAGTGTGCTGGCGTTTGACCAAGAGCGTCTGGAGCGGAAGATCCTGG
CCCTCAGGCAGGCGCGGCGGCCGGTGCCCCCAGAAGTGGCCCAGCAGTACCAGGACATCATGCAACGCAG
CCAGTGGCAGAGGGCACAGCTGGAGCAGGGGGGTGTGGGCATCCGACGGGAATACGCAGCCCAGCTGGAG
CGGCAGCTGCAGTTCTACACGGAGGCTGCCCGGCGCCTGGGCAACGATGGCAGCAGGGATGCTGCAAAGG
AGGCGCTCTATAGGCGGAATCTGGTAGAGAGTGAGCTGCAGCGGCTCCGCAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209105 protein sequence
Red=Cloning site Green=Tags(s)

MHKRKGPPGPPGRGAAAARQLGLLVDLSPDGLMIPEDGANDEELEAEFLALVGGQPPALEKLKGKGPLPM
EAIEKMASLCMRDPDEDEEEGTDEDDLEADDDLLAELNEVLGEEQKASETPPPVAQPKPEAPHPGLETTL
QERLALYQTAIESARQAGDSAKMRRYDRGLKTLENLLASIRKGNAIDEADIPPPVAIGKGPASTPTYSPA
PTQPAPRIASAPEPRVTLEGPSATAPASSPGLAKPQMPPGPCSPGPLAQLQSRQRDYKLAALHAKQQGDT
TAAARHFRVAKSFDAVLEALSRGEPVDLSCLPPPPDQLPPDPPSPPSQPPTPATAPSTTEVPPPPRTLLE
ALEQRMERYQVAAAQAKSKGDQRKARMHERIVKQYQDAIRAHKAGRAVDVAELPVPPGFPPIQGLEATKP
TQQSLVGVLETAMKLANQDEGPEDEEDEVPKKQNSPVAPTAQPKAPPSRTPQSGSAPTAKAPPKATSTRA
QQQLAFLEGRKKQLLQAALRAKQKNDVEGAKMHLRQAKGLEPMLEASRNGLPVDITKVPPAPVNKDDFAL
VQRPGPGLSQEAARRYGELTKLIRQQHEMCLNHSNQFTQLGNITETTKFEKLAEDCKRSMDILKQAFVRG
LPTPTARFEQRTFSVIKIFPDLSSNDMLLFIVKGINLPTPPGLSPGDLDVFVRFDFPYPNVEEAQKDKTS
VIKNTDSPEFKEQFKLCINRSHRGFRRAIQTKGIKFEVVHKGGLFKTDRVLGTAQLKLDALEIACEVREI
LEVLDGRRPTGGRLEVMVRIREPLTAQQLETTTERWLVIDPVPAAVPTQVAGPKGKAPPVPAPARESGNR
SARPLHSLSVLAFDQERLERKILALRQARRPVPPEVAQQYQDIMQRSQWQRAQLEQGGVGIRREYAAQLE
RQLQFYTEAARRLGNDGSRDAAKEALYRRNLVESELQRLRR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017721
ORF Size 2853 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017721.5
RefSeq Size 3644 bp
RefSeq ORF 2856 bp
Locus ID 54862
UniProt ID Q6P1N0
Cytogenetics 19p13.12
Domains C2, DM14
MW 104.1 kDa
Summary This gene encodes a transcriptional repressor that binds to a conserved 14-bp 5'-repressor element and regulates expression of the 5-hydroxytryptamine (serotonin) receptor 1A gene in neuronal cells. The DNA binding and transcriptional repressor activities of the protein are inhibited by calcium. A mutation in this gene results in a nonsyndromic form of cognitive disability (MRT3). provided by RefSeq, Jul 2017
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Resources
"NM_017721" in other vectors (7)
SKU Description Size Price
RC209105L1 Lenti ORF clone of Human coiled-coil and C2 domain containing 1A (CC2D1A), Myc-DDK-tagged 10 ug
$1,171.00
RC209105L2 Lenti ORF clone of Human coiled-coil and C2 domain containing 1A (CC2D1A), mGFP tagged 10 ug
$1,171.00
RC209105L3 Lenti ORF clone of Human coiled-coil and C2 domain containing 1A (CC2D1A), Myc-DDK-tagged 10 ug
$1,171.00
RC209105L4 Lenti ORF clone of Human coiled-coil and C2 domain containing 1A (CC2D1A), mGFP tagged 10 ug
$1,171.00
RG209105 CC2D1A (tGFP-tagged) - Human coiled-coil and C2 domain containing 1A (CC2D1A) 10 ug
$789.00 $1,071.00
SC114001 CC2D1A (untagged)-Human coiled-coil and C2 domain containing 1A (CC2D1A) 10 ug
$871.00
SC318410 CC2D1A (untagged)-Human coiled-coil and C2 domain containing 1A (CC2D1A) 10 ug
$959.00

Plasmid Map for RC209105

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209105
Circular map for RC209105

file-alt Citations

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