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MRPL14 (NM_032111) Human Tagged ORF Clone

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SKU
RC209019
MRPL14 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L14 (MRPL14), nuclear gene encoding mitochondrial protein
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "MRPL14 or NM_032111"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MRPL14
Synonyms L14mt; L32mt; MRP-L14; MRP-L32; MRPL32; RMPL32; RPML32
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC209019 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTTCTTTACTGGGCTCTGGGGCCCCTTCACCTGTGTAAGCAGAGTGCTGAGCCATCACTGTTTCA
GCACCACTGGGAGTCTGAGTGCGATTCAGAAGATGACGCGGGTACGAGTGGTGGACAACAGTGCCCTGGG
GAACAGCCCATACCATCGGGCTCCTCGCTGCATCCATGTCTATAAGAAGAATGGAGTGGGCAAGGTGGGC
GACCAGATACTACTGGCCATCAAGGGACAGAAGAAAAAGGCGCTCATTGTGGGGCACTGCATGCCTGGCC
CCCGAATGACCCCCAGATTCGACTCCAACAACGTGGTCCTCATTGAGGACAACGGGAACCCTGTGGGGAC
ACGAATTAAGACACCCATCCCCACCAGCCTGCGCAAGCGGGAAGGCGAGTATTCCAAGGTGCTGGCCATT
GCTCAGAACTTTGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC209019 protein sequence
Red=Cloning site Green=Tags(s)

MAFFTGLWGPFTCVSRVLSHHCFSTTGSLSAIQKMTRVRVVDNSALGNSPYHRAPRCIHVYKKNGVGKVG
DQILLAIKGQKKKALIVGHCMPGPRMTPRFDSNNVVLIEDNGNPVGTRIKTPIPTSLRKREGEYSKVLAI
AQNFV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032111
ORF Size 435 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032111.4
RefSeq Size 791 bp
RefSeq ORF 438 bp
Locus ID 64928
UniProt ID Q6P1L8, A0A024RD78
Cytogenetics 6p21.1
Domains Ribosomal_L14
MW 15.9 kDa
Summary This nuclear gene encodes a protein component of the 39S subunit of the mitochondrial ribosome. A pseudogene of this gene is found on chromosome 17. provided by RefSeq, Jan 2016
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"NM_032111" in other vectors (6)
SKU Description Size Price
RC209019L1 Lenti ORF clone of Human mitochondrial ribosomal protein L14 (MRPL14), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$450.00
RC209019L2 Lenti ORF clone of Human mitochondrial ribosomal protein L14 (MRPL14), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$450.00
RC209019L3 Lenti ORF clone of Human mitochondrial ribosomal protein L14 (MRPL14), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$450.00
RC209019L4 Lenti ORF clone of Human mitochondrial ribosomal protein L14 (MRPL14), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$450.00
RG209019 MRPL14 (tGFP-tagged) - Human mitochondrial ribosomal protein L14 (MRPL14), nuclear gene encoding mitochondrial protein 10 ug
$489.00
SC120707 MRPL14 (untagged)-Human mitochondrial ribosomal protein L14 (MRPL14), nuclear gene encoding mitochondrial protein 10 ug
$150.00

Plasmid Map for RC209019

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC209019
Circular map for RC209019

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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