COPG (COPG1) (NM_016128) Human Tagged ORF Clone

SKU RC209018

COPG1 (Myc-DDK-tagged)-Human coatomer protein complex, subunit gamma (COPG)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: COPG
• Synonyms: COPG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209018 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTGAAGAAATTCGACAAGAAGGATGAGGAGTCAGGTGGAGGCTCCAACCCATTCCAGCACCTTGAGA; AGAGTGCGGTACTCCAGGAGGCCCGTGTATTTAATGAAACTCCCATCAACCCTCGGAAATGTGCCCACAT; CCTCACCAAGATTCTTTATCTCATAAACCAGGGGGAGCACCTGGGGACCACGGAAGCGACCGAGGCCTTC; TTTGCCATGACCAAGCTCTTTCAGTCCAATGATCCCACACTCCGTCGGATGTGCTACTTGACCATCAAGG; AGATGTCTTGCATTGCAGAGGATGTCATCATTGTCACCAGCAGCCTAACAAAAGACATGACTGGGAAAGA; AGACAACTACCGGGGCCCGGCCGTGCGAGCCCTCTGCCAGATCACTGATAGCACCATGCTGCAGGCTATT; GAGCGCTACATGAAACAAGCCATTGTGGACAAGGTGCCCAGTGTCTCCAGCTCTGCCCTCGTGTCTTCCT; TGCACCTGCTGAAGTGCAGCTTTGACGTGGTCAAGCGCTGGGTGAATGAGGCTCAGGAGGCAGCATCCAG; TGATAACATCATGGTCCAGTACCACGCACTAGGGCTCCTGTACCATGTGCGTAAGAATGACCGCCTAGCC; GTCAATAAGATGATCAGCAAGGTCACACGGCATGGCCTTAAGTCTCCCTTTGCCTACTGCATGATGATCC; GGGTGGCCAGCAAGCAGCTGGAAGAGGAGGATGGCAGCCGTGACAGCCCACTGTTTGACTTCATCGAGAG; CTGCTTGCGCAACAAGCACGAGATGGTGGTGTATGAAGCCGCCTCGGCCATCGTCAATCTGCCAGGCTGC; AGTGCCAAAGAGCTGGCCCCGGCTGTGTCAGTGCTCCAGCTTTTCTGCAGCTCACCCAAGGCTGCTCTCC; GCTATGCTGCTGTTCGTACCCTCAATAAGGTTGCCATGAAGCATCCGTCAGCTGTGACAGCTTGTAATCT; GGATCTGGAGAACCTGGTCACAGATTCAAACCGCAGCATTGCCACGCTGGCCATCACCACCCTCCTTAAG; ACGGGCAGCGAGAGCAGCATCGACCGCCTCATGAAGCAGATCTCCTCCTTCATGTCAGAAATCTCGGATG; AATTCAAGGTGGTGGTTGTCCAGGCCATCAGTGCCCTGTGTCAGAAATATCCTCGCAAACACGCCGTCCT; TATGAACTTCCTGTTCACCATGCTGCGGGAAGAGGGTGGCTTTGAGTATAAGCGCGCTATCGTGGACTGC; ATCATCAGCATCATTGAAGAGAACTCAGAGAGCAAGGAGACAGGGCTGTCACATCTGTGCGAGTTCATCG; AGGACTGCGAGTTCACAGTGCTGGCCACCCGTATTCTACATCTCCTGGGCCAGGAGGGGCCCAAGACCAC; CAATCCCTCAAAGTACATCCGCTTCATCTATAACCGAGTGGTCTTGGAGCATGAGGAGGTCCGGGCAGGT; GCTGTGAGTGCTCTGGCGAAGTTTGGAGCCCAGAATGAAGAGATGTTACCCAGTATCTTGGTGTTGCTGA; AGAGGTGTGTGATGGATGATGACAATGAAGTAAGGGACCGAGCCACCTTCTACCTAAATGTCCTGGAGCA; GAAGCAGAAGGCCCTTAATGCAGGCTATATCCTAAATGGTCTGACTGTGTCCATCCCTGGTCTGGAGAGG; GCTCTGCAGCAGTACACTCTAGAACCATCAGAAAAACCTTTTGACCTCAAGTCTGTGCCCCTGGCCACGG; CGCCCATGGCAGAGCAGAGAACAGAAAGTACCCCCATCACAGCAGTCAAACAGCCTGAGAAAGTGGCAGC; TACCAGGCAGGAGATCTTCCAGGAGCAGTTGGCAGCAGTGCCAGAGTTCCGCGGTCTTGGGCCCCTCTTC; AAGTCCTCGCCTGAGCCCGTGGCCCTCACCGAGTCAGAGACGGAGTATGTCATCCGCTGCACCAAACACA; CCTTCACCAACCACATGGTTTTTCAGTTTGACTGCACAAACACACTCAATGACCAGACCTTGGAGAATGT; CACAGTGCAGATGGAGCCCACTGAGGCCTATGAGGTGCTCTGTTACGTGCCTGCCCGGAGCCTGCCCTAC; AACCAGCCCGGGACCTGCTACACACTGGTGGCACTGCCCAAAGAAGACCCCACAGCTGTGGCCTGCACAT; TCAGCTGCATGATGAAGTTCACTGTCAAGGACTGTGATCCCACCACTGGGGAGACTGATGACGAAGGCTA; TGAGGATGAGTATGTGCTGGAAGATCTGGAAGTTACTGTAGCTGATCACATTCAAAAGGTCATGAAACTG; AACTTCGAAGCAGCCTGGGATGAGGTAGGGGATGAATTTGAGAAGGAGGAAACGTTCACCTTGTCTACCA; TCAAGACACTTGAAGAGGCTGTGGGTAATATTGTGAAGTTCTTGGGAATGCACCCTTGTGAGAGGTCAGA; CAAAGTGCCGGATAACAAGAACACCCACACGTTGCTCCTGGCTGGTGTGTTCCGGGGTGGTCATGACATC; CTGGTGCGCTCCCGGCTGCTGCTTTTGGACACAGTGACAATGCAGGTGACAGCCAGAAGTTTGGAGGAGC; TGCCAGTAGACATCATCTTGGCATCTGTGGGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209018 protein sequence; Red=Cloning site Green=Tags(s); ; MLKKFDKKDEESGGGSNPFQHLEKSAVLQEARVFNETPINPRKCAHILTKILYLINQGEHLGTTEATEAF; FAMTKLFQSNDPTLRRMCYLTIKEMSCIAEDVIIVTSSLTKDMTGKEDNYRGPAVRALCQITDSTMLQAI; ERYMKQAIVDKVPSVSSSALVSSLHLLKCSFDVVKRWVNEAQEAASSDNIMVQYHALGLLYHVRKNDRLA; VNKMISKVTRHGLKSPFAYCMMIRVASKQLEEEDGSRDSPLFDFIESCLRNKHEMVVYEAASAIVNLPGC; SAKELAPAVSVLQLFCSSPKAALRYAAVRTLNKVAMKHPSAVTACNLDLENLVTDSNRSIATLAITTLLK; TGSESSIDRLMKQISSFMSEISDEFKVVVVQAISALCQKYPRKHAVLMNFLFTMLREEGGFEYKRAIVDC; IISIIEENSESKETGLSHLCEFIEDCEFTVLATRILHLLGQEGPKTTNPSKYIRFIYNRVVLEHEEVRAG; AVSALAKFGAQNEEMLPSILVLLKRCVMDDDNEVRDRATFYLNVLEQKQKALNAGYILNGLTVSIPGLER; ALQQYTLEPSEKPFDLKSVPLATAPMAEQRTESTPITAVKQPEKVAATRQEIFQEQLAAVPEFRGLGPLF; KSSPEPVALTESETEYVIRCTKHTFTNHMVFQFDCTNTLNDQTLENVTVQMEPTEAYEVLCYVPARSLPY; NQPGTCYTLVALPKEDPTAVACTFSCMMKFTVKDCDPTTGETDDEGYEDEYVLEDLEVTVADHIQKVMKL; NFEAAWDEVGDEFEKEETFTLSTIKTLEEAVGNIVKFLGMHPCERSDKVPDNKNTHTLLLAGVFRGGHDI; LVRSRLLLLDTVTMQVTARSLEELPVDIILASVG; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016128
• ORF Size: 2622 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016128.4
• RefSeq Size: 3114 bp
• RefSeq ORF: 2625 bp
• Locus ID: 22820
• UniProt ID: Q9Y678
• Cytogenetics: 3q21.3
• Domains: Adaptin_N
• Protein Families: Druggable Genome
• MW: 97.7 kDa
• Summary: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis (By similarity).[UniProtKB/Swiss-Prot Function]