COPG (COPG1) (NM_016128) Human Untagged Clone

SKU SC108411

COPG1 (untagged)-Human coatomer protein complex, subunit gamma (COPG)

Product Data

• Type: Human Untagged Clone
• Target Symbol: COPG
• Synonyms: COPG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108411 sequence for NM_016128 edited (data generated by NextGen Sequencing); ATGTTGAAGAAATTCGACAAGAAGGATGAGGAGTCAGGTGGAGGCTCCAACCCATTCCAG; CACCTTGAGAAGAGTGCGGTACTCCAGGAGGCCCGTGTATTTAATGAAACTCCCATCAAC; CCTCGGAAATGTGCCCACATCCTCACCAAGATTCTTTATCTCATAAACCAGGGGGAGCAC; CTGGGGACCACGGAAGCGACCGAGGCCTTCTTTGCCATGACCAAGCTCTTTCAGTCCAAT; GATCCCACACTCCGTCGGATGTGCTACTTGACCATCAAGGAGATGTCTTGCATTGCAGAG; GATGTCATCATTGTCACCAGCAGCCTAACAAAAGACATGACTGGGAAAGAAGACAACTAC; CGGGGCCCGGCCGTGCGAGCCCTCTGCCAGATCACTGATAGCACCATGCTGCAGGCTATT; GAGCGCTACATGAAACAAGCCATTGTGGACAAGGTGCCCAGTGTCTCCAGCTCTGCCCTC; GTGTCTTCCTTGCACCTGCTGAAGTGCAGCTTTGACGTGGTCAAGCGCTGGGTGAATGAG; GCTCAGGAGGCAGCATCCAGTGATAACATCATGGTCCAGTACCACGCACTAGGGCTCCTG; TACCATGTGCGTAAGAATGACCGCCTAGCCGTCAATAAGATGATCAGCAAGGTCACACGG; CATGGCCTTAAGTCTCCCTTTGCCTACTGCATGATGATCCGGGTGGCCAGCAAGCAGCTG; GAAGAGGAGGATGGCAGCCGTGACAGCCCACTGTTTGACTTCATCGAGAGCTGCTTGCGC; AACAAGCACGAGATGGTGGTGTATGAAGCCGCCTCGGCCATCGTCAATCTGCCAGGCTGC; AGTGCCAAAGAGCTGGCCCCGGCTGTGTCAGTGCTCCAGCTTTTCTGCAGCTCACCCAAG; GCTGCTCTCCGCTATGCTGCTGTTCGTACCCTCAATAAGGTTGCCATGAAGCATCCGTCA; GCTGTGACAGCTTGTAATCTGGATCTGGAGAACCTGGTCACAGATTCAAACCGCAGCATT; GCCACGCTGGCCATCACCACCCTCCTTAAGACGGGCAGCGAGAGCAGCATCGACCGCCTC; ATGAAGCAGATCTCCTCCTTCATGTCAGAAATCTCGGATGAATTCAAGGTGGTGGTTGTC; CAGGCCATCAGTGCCCTGTGTCAGAAATATCCTCGCAAACACGCCGTCCTTATGAACTTC; CTGTTCACCATGCTGCGGGAAGAGGGTGGCTTTGAGTATAAGCGCGCTATCGTGGACTGC; ATCATCAGCATCATTGAAGAGAACTCAGAGAGCAAGGAGACAGGGCTGTCACATCTGTGC; GAGTTCATCGAGGACTGCGAGTTCACAGTGCTGGCCACCCGTATTCTACATCTCCTGGGC; CAGGAGGGGCCCAAGACCACCAATCCCTCAAAGTACATCCGCTTCATCTATAACCGAGTG; GTCTTGGAGCATGAGGAGGTCCGGGCAGGTGCTGTGAGTGCTCTGGCGAAGTTTGGAGCC; CAGAATGAAGAGATGTTACCCAGTATCTTGGTGTTGCTGAAGAGGTGTGTGATGGATGAT; GACAATGAAGTAAGGGACCGAGCCACCTTCTACCTAAATGTCCTGGAGCAGAAGCAGAAG; GCCCTTAATGCAGGCTATATCCTAAATGGTCTGACTGTGTCCATCCCTGGTCTGGAGAGG; GCTCTGCAGCAGTACACTCTAGAACCATCAGAAAAACCTTTTGACCTCAAGTCTGTGCCC; CTGGCCACGGCGCCCATGGCAGAGCAGAGAACAGAAAGTACCCCCATCACAGCAGTCAAA; CAGCCTGAGAAAGTGGCAGCTACCAGGCAGGAGATCTTCCAGGAGCAGTTGGCAGCAGTG; CCAGAGTTCCGCGGTCTTGGGCCCCTCTTCAAGTCCTCGCCTGAGCCCGTGGCCCTCACC; GAGTCAGAGACGGAGTATGTCATCCGCTGCACCAAACACACCTTCACCAACCACATGGTT; TTTCAGTTTGACTGCACAAACACACTCAATGACCAGACCTTGGAGAATGTCACAGTGCAG; ATGGAGCCCACTGAGGCCTATGAGGTGCTCTGTTACGTGCCTGCCCGGAGCCTGCCCTAC; AACCAGCCCGGGACCTGCTACACACTGGTGGCACTGCCCAAAGAAGACCCCACAGCTGTG; GCCTGCACATTCAGCTGCATGATGAAGTTCACTGTCAAGGACTGTGATCCCACCACTGGG; GAGACTGATGACGAAGGCTATGAGGATGAGTATGTGCTGGAAGATCTGGAAGTTACTGTA; GCTGATCACATTCAAAAGGTCATGAAACTGAACTTCGAAGCAGCCTGGGATGAGGTAGGG; GATGAATTTGAGAAGGAGGAAACGTTCACCTTGTCTACCATCAAGACACTTGAAGAGGCT; GTGGGTAATATTGTGAAGTTCTTGGGAATGCACCCTTGTGAGAGGTCAGACAAAGTGCCG; GATAACAAGAACACCCACACGTTGCTCCTGGCTGGTGTGTTCCGGGGTGGTCATGACATC; CTGGTGCGCTCCCGGCTGCTGCTTTTGGACACAGTGACAATGCAGGTGACAGCCAGAAGT; TTGGAGGAGCTGCCAGTAGACATCATCTTGGCATCTGTGGGATAA; ; Clone variation with respect to NM_016128.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016128 unedited; NTGTTCGAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCGTT; GCTGCATTGCGCCCCACCGACTCCACTATGTTGAAGAAATTCGACAAGAAGGATGAGGAG; TCAGGTGGAGGCTCCAACCCATTCCAGCACCTTGAGAAGAGTGCGGTACTCCAGGAGGCC; CGTGTATTTAATGAAACTCCCATCAACCCTCGGAAATGTGCCCACATCCTCACCAAGATT; CTTTATCTCATAAACCAGGGGGAGCACCTGGGGACCACGGAAGCGACCGAGGCCTTCTTT; GCCATGACCAAGCTCTTTCAGTCCAATGATCCCACACTCCGTCGGATGTGCTACTTGACC; ATCAAGGAGATGTCTTGCATTGCAGAGGATGTCATCATTGTCACCAGCAGCCTAACAAAA; GACATGACTGGGAAAGAAGACAACTACCGGGGCCCGGCCGTGCGAGCCCTCTGCCAGATC; ACTGATAGCACCATGCTGCAGGCTATTGAGCGCTACATGAAACAAGCCATTGTGGACAAG; GTGCCCAGTGTCTCCAGCTCTGCCCTCGTGTCTTCCTTGCACCTGCTGAAGTGCAGCTTT; GACGTGGTCAAGCGCTGGGTGAATGAGGCTCAGGAGGCAGCATCCAGTGATAACATCATG; GTCCAGTACCACGCACTAGGGCTCCTGTACCATGTGCGTAAGAATGACCGCCTAGCCGTC; AATAAGATGATCAGCAAGGTCACACGGCATGGCCTTAAGTCTCCCTTTGCCTACTGCATG; ATGATCCGGGTGGCCAGCAAGCAGCTGGAAGAGGAGGATGGCAGCCGTGACAGCCCACTG; TTTGACTTCATCGAGAGCTGCTTGCGCAACAGCACGAGATGGTGGTGTATGAAGCCGNNC; TCGCCATCGTCAATCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016128 unedited; TTGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCATAAATACATG; CTGATTTATTACAGGGATAAGATGGTTTCTTGGGGGATAGATTCAAGAGGAGTTGAGAAT; GTTTTATTCATTTACAATGTCCCTTTCCTGGAAGGGTGGACAGCAAGATTTAGGACAAGC; TAAAATCATCCCCTATTTAAAAAAAAAAAAAAAAAAAGTCACCAGCAAGTATTCCCGGGT; GGGAGGTGGGAGCAGAATAAAAAAAAATCTGCAATGATTCCTAATTGTTTTTCAATACAG; AAGCTTGGGAAGGGGTTTCTGCCAGTTTCATGAGGAAGGCACAACTTCCAGGTAGTGTTG; GGGAAGGGTATGAGGTCCTATGCAGGCTGGCCTCTTATCCCACAGATGCCAAGATGATGT; CTACTGGCAGCTCCTCCAAACTTCTGGCTGTCACCTGCATTGTCACTGTGTCCAAAAGCA; GCAGCCGGGAGCGCACCAGGATGTCATGACCACCCCGGAACACACCAGCCAGGAGCAACG; TGTGGGTGTTCTTGTTATCCGGCACTTTGTCTGACCTCTACAAGGGTGCATTCCCAAGAA; CTTCACAATATTACCCACAGCCTCTTCAAGTGTCTTGATGGTAGACAAGGTGAACGTTTC; CTCCTTCTCAAATTCATCCCCTACCTCATCCCAGGCTGCTTCGAAGTTCAGTTTCATGAC; CTTTTGAATGTGATCAGCTACAGTAACTTNCAGATCTTCCAGCACATACTCATCCTCATA; GCCTTCGTCATCAGTCTCCCCAGTGGTGGGATCACAGTCCTTGACAGTGAACTTCATCAT; GCAGCTGAATGTGCAGGCCACAGCTGTGGGGGTCTTCTTTGGGCANTGCCACAGTGTGTA; NCAGGTCCCGGNNCTGGTGTAGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_016128
• Insert Size: 3180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016128.3, NP_057212.1
• RefSeq Size: 3114 bp
• RefSeq ORF: 2625 bp
• Locus ID: 22820
• UniProt ID: Q9Y678
• Cytogenetics: 3q21.3
• Domains: Adaptin_N
• Protein Families: Druggable Genome
• Summary: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis (By similarity).[UniProtKB/Swiss-Prot Function]